Extended Photon Counting Histogram and Fluorescence Intensity Distribution Analysis Approaches for Optically Biased Photon Counting Statistics

2006 ◽  
Vol 110 (19) ◽  
pp. 9667-9673 ◽  
Author(s):  
Fanbo Meng ◽  
Hui Ma
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Distribution ◽  
Photon Counting ◽  
Distribution Analysis ◽  
Fluorescence Intensity Distribution Analysis ◽  
Photon Counting Histogram ◽  
Counting Statistics ◽  
Photon Counting Statistics

scholarly journals Two-Dimensional Fluorescence Intensity Distribution Analysis: Theory and Applications

2000 ◽  
Vol 78 (4) ◽  
pp. 1703-1713 ◽  
Author(s):  
Peet Kask ◽  
Kaupo Palo ◽  
Nicolas Fay ◽  
Leif Brand ◽  
Ülo Mets ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Distribution ◽  
Distribution Analysis ◽  
Two Dimensional ◽  
Analysis Theory ◽  
Fluorescence Intensity Distribution Analysis

Single-Molecule Detection Technologies in Miniaturized High-Throughput Screening: Fluorescence Intensity Distribution Analysis

2003 ◽  
Vol 8 (1) ◽  
pp. 19-33 ◽  
Author(s):  
Ulrich Haupts ◽  
Martin Rüdiger ◽  
Stephen Ashman ◽  
Sandra Turconi ◽  
Ryan Bingham ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Molecule ◽  
Fluorescence Intensity ◽  
Intensity Distribution ◽  
High Throughput Screening ◽  
Biological Data ◽  
Single Molecule Detection ◽  
Distribution Analysis ◽  
Fluorescence Intensity Distribution Analysis ◽  
Detection Technologies

Single-molecule detection technologies are becoming a powerful readout format to support ultra-high-throughput screening. These methods are based on the analysis of fluorescence intensity fluctuations detected from a small confocal volume element. The fluctuating signal contains information about the mass and brightness of the different species in a mixture. The authors demonstrate a number of applications of fluorescence intensity distribution analysis (FIDA), which discriminates molecules by their specific brightness. Examples for assays based on brightness changes induced by quenching/dequenching of fluorescence, fluorescence energy transfer, and multiple-binding stoichiometry are given for important drug targets such as kinases and proteases. FIDA also provides a powerful method to extract correct biological data in the presence of compound fluorescence. ( Journal of Biomolecular Screening 2003:19-33)

scholarly journals Development of a 1-μl Scale Assay for Mitogen-Activated Kinase Kinase 7 Using 2-D Fluorescence Intensity Distribution Analysis Anisotropy

2002 ◽  
Vol 7 (5) ◽  
pp. 419-428 ◽  
Author(s):  
Penny A. Wright ◽  
Helen F. Boyd ◽  
Richard C. Bethell ◽  
Michael Busch ◽  
Phillip Gribbon ◽  
...  
Keyword(s):  
Steady State ◽  
Fluorescence Intensity ◽  
Intensity Distribution ◽  
Fluorescence Polarization ◽  
High Throughput Screening ◽  
Phosphorylation Site ◽  
Mitogen Activated Protein Kinase ◽  
Superior Performance ◽  
Distribution Analysis ◽  
Fluorescence Intensity Distribution Analysis

This paper describes the development of a robust, miniaturizable, and quantitative fluorescence-based assay for mitogen-activated protein kinase kinase 7 (MKK7). As a first step, the basic steady-state kinetics of the MKK7-catalyzed phosphorylation of c-Jun N-terminal kinases (JNKs) 1, 2, and 3 were defined using standard radiometric methods. Subsequently, the authors found that in addition to the holo JNKs, a series of novel small peptides (based on the region around the JNK phosphorylation site) are also substrates, provided that these were prephosphorylated on the Y residue of the TPY motif. One of these peptide substrates was used in the development of a fluorescence polarization-based assay using an antibody as a sensor. The assay was successfully miniaturized for use with conventional fluorescence polarization (FP) reader technology in 8.5 μl and on the single μl scale using Evotec proprietary 2-dimensional fluorescence intensity distribution analysis (2D-FIDA) anisotropy and liquid handling technology. The steady-state kinetic parameters derived using the FP or 2D-FIDA anisotropy format assays correlated well with those generated using a radiometric assay. Moreover, the quantitative sensitivity to known inhibitors was maintained independent of the format and assay volume. In addition, the authors found that the 2D-FIDA anisotropy assay exhibited superior performance statistics (typical Z' = ~0.5) relative to conventional FP (typical Z' = 0.3) and yielded the additional benefit of order-of-magnitude savings in terms of reagent costs. The 2D-FIDA anisotropy assay was used to carry out a successful high-throughput screening in 1-μl final volume against company file compounds.

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