Electrochemical Impedance Sensing of DNA Hybridization on Conducting Polymer Film-Modified Diamond

2005 ◽  
Vol 109 (28) ◽  
pp. 13611-13618 ◽  
Author(s):  
Huiru Gu ◽  
Xiao di Su ◽  
Kian Ping Loh
Keyword(s):  
Polymer Film ◽  
Conducting Polymer ◽  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Impedance Sensing

Label-free electrochemical impedance sensing of DNA hybridization based on functionalized graphene sheets

2011 ◽  
Vol 47 (6) ◽  
pp. 1743-1745 ◽  
Author(s):  
Yuwei Hu ◽  
Fenghua Li ◽  
Xiaoxue Bai ◽  
Dan Li ◽  
Shucheng Hua ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Graphene Sheets ◽  
Functionalized Graphene ◽  
Label Free ◽  
Impedance Sensing

scholarly journals Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes

Electronics ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 347 ◽  
Author(s):  
Maria Chiriacò ◽  
Ilaria Parlangeli ◽  
Fausto Sirsi ◽  
Palmiro Poltronieri ◽  
Elisabetta Primiceri
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Foodborne Pathogen ◽  
High Sensitivity ◽  
Primary Objective ◽  
Field Application ◽  
Impedance Sensing ◽  
Sensing Platform

A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks, due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics, but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 × 103 cfu/mL to 1 × 102 with a Limit of Detection (LoD) of 5.5 cfu/mL.

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