Facile and ultrasensitive electrochemical impedance sensing for formaldehyde based on silver ions doped in controllable and homogeneous silica microspheres

2019 ◽  
Vol 284 ◽  
pp. 657-662 ◽  
Author(s):  
Hongbo Li ◽  
Yanli Li ◽  
Mengya Li ◽  
Lingqiu Xu ◽  
Jing Li
Electronics ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 347 ◽  
Author(s):  
Maria Chiriacò ◽  
Ilaria Parlangeli ◽  
Fausto Sirsi ◽  
Palmiro Poltronieri ◽  
Elisabetta Primiceri

A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks, due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics, but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 × 103 cfu/mL to 1 × 102 with a Limit of Detection (LoD) of 5.5 cfu/mL.


Cytotherapy ◽  
2011 ◽  
Vol 13 (9) ◽  
pp. 1074-1089 ◽  
Author(s):  
Michael Angstmann ◽  
Irena Brinkmann ◽  
Karen Bieback ◽  
Dirk Breitkreutz ◽  
Christian Maercker

2011 ◽  
Vol 47 (6) ◽  
pp. 1743-1745 ◽  
Author(s):  
Yuwei Hu ◽  
Fenghua Li ◽  
Xiaoxue Bai ◽  
Dan Li ◽  
Shucheng Hua ◽  
...  

Author(s):  
Maria Serena Chiriacò ◽  
Ilaria Parlangeli ◽  
Palmiro Poltronieri ◽  
Elisabetta Primiceri

A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 x 103 cfu/ml to 1 x 102 with a Limit of Detection (LoD) of 5.5 cfu/ml.


2020 ◽  
Vol 85 (10) ◽  
pp. 1317-1328
Author(s):  
López Forero ◽  
Ana Loperena ◽  
Ivana Lehr ◽  
Lorena Brugnoni ◽  
Silvana Saidman

A duplex coating was formed under potentiostatic conditions on magnesium alloy AZ91D in order to improve its corrosion resistance in a simulated physiological environment. The first layer was formed by anodization at low potentials in molybdate solution. The outer layer was a PPy film electrosynthesized in sodium salicylate solution. The conditions of the formation were determined to obtain a layer with globular morphology. The bilayer was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The corrosion protection properties of the coatings were examined in Ringer solution by monitoring the open circuit potential (OCP), polarization techniques, and electrochemical impedance spectroscopy (EIS). The obtained results showed that the bilayer improves the corrosion resistance of the substrate. Moreover, the duplex coating presented better anticorrosive properties than a single PPy film. Afterwards, the bilayer was modified by cementation of silver ions from a solution containing AgNO3. The modified electrode exhibited good antibacterial properties.


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