Assignments and Conformational Dependencies of the Amide III Peptide Backbone UV Resonance Raman Bands

2004 ◽  
Vol 108 (49) ◽  
pp. 19020-19028 ◽  
Author(s):  
Alexander V. Mikhonin ◽  
Zeeshan Ahmed ◽  
Anatoli Ianoul ◽  
Sanford A. Asher
Keyword(s):  
Resonance Raman ◽  
Peptide Backbone ◽  
Uv Resonance Raman

scholarly journals Correlation of TrpGly and GlyTrp Rotamer Structure with W7 and W10 UV Resonance Raman Modes and Fluorescence Emission Shifts

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Azaria Solomon Eisenberg ◽  
Laura J. Juszczak
Keyword(s):  
Stark Effect ◽  
Resonance Raman ◽  
Fluorescence Emission ◽  
High Energy ◽  
Appreciable Amount ◽  
Peptide Backbone ◽  
Band Ratio ◽  
Uv Resonance Raman ◽  
Raman Modes ◽  
Discrete Values

Tryptophyl glycine (TrpGly) and glycyl tryptophan (GlyTrp) dipeptides at pH 5.5 and pH 9.3 show a pattern of fluorescence emission shifts with the TrpGly zwitterion emission solely blue shifted. This pattern is matched by shifts in the UV resonance Raman (UVRR) W10 band position and the W7 Fermi doublet band ratio. Ab initio calculations show that the 1340 cm−1 band of the W7 doublet is composed of three modes, two of which determine the W7 band ratios for the dipeptides. Molecular dynamics simulations show that the dipeptides take on two conformations: one with the peptide backbone extended; one with the backbone curled over the indole. The dihedral angle critical to these conformations is χ1 and takes on three discrete values. Only the TrpGly zwitterion spends an appreciable amount of time in the extended backbone conformation as this is stabilized by two hydrogen bonds with the terminal amine cation. According to a Stark effect model, a positive charge near the pyrrole keeps the 1La transition at high energy, limiting fluorescence emission red shift, as observed for the TrpGly zwitterion. The hydrogen bond stabilized backbone provides a rationale for the Cmethylene-Cα-Ccarbonyl W10 symmetric stretch that is unique to the TrpGly zwitterion.

Simultaneous Observation of Peptide Backbone Lipid Solvation and α-Helical Structure by Deep-UV Resonance Raman Spectroscopy

ChemBioChem ◽  
2011 ◽  
Vol 12 (14) ◽  
pp. 2125-2128 ◽  
Author(s):  
Christopher M. Halsey ◽  
Jian Xiong ◽  
Olayinka O. Oshokoya ◽  
Jeanette A. Johnson ◽  
Sandip Shinde ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Helical Structure ◽  
Simultaneous Observation ◽  
Peptide Backbone ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Deep Uv

Deep-UV Resonance Raman Analysis of theRhodobacter capsulatusCytochromebc1Complex Reveals a Potential Marker for the Transmembrane Peptide Backbone

Biochemistry ◽  
2011 ◽  
Vol 50 (30) ◽  
pp. 6531-6538 ◽  
Author(s):  
Christopher M. Halsey ◽  
Olayinka O. Oshokoya ◽  
Renee D. Jiji ◽  
Jason W. Cooley
Keyword(s):  
Resonance Raman ◽  
Peptide Backbone ◽  
Raman Analysis ◽  
Potential Marker ◽  
Uv Resonance Raman ◽  
Deep Uv

UV resonance Raman signatures of phonon-allowed absorptions and phonon-driven bubble formation

2015 ◽  
Author(s):  
Hans D. Hallen ◽  
Adam Willitsford ◽  
Reagan Weeks ◽  
C. Russell Philbrick
Keyword(s):  
Bubble Formation ◽  
Resonance Raman ◽  
Uv Resonance Raman

UV resonance Raman spectroscopic study of photodegradation of hardwood and softwood lignins by UV laser

Holzforschung ◽  
2008 ◽  
Vol 62 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Krishna K. Pandey ◽  
Tapani Vuorinen
Keyword(s):  
Lignin Degradation ◽  
Laser Energy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Photochemical Degradation ◽  
Raman Spectroscopic ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Ion Laser ◽  
Chir Pine

Abstract The effect of laser irradiation (Ar+ ion laser, 244 nm) on photodegradation of lignin in silver birch and rubberwood as hardwoods and spruce and chir pine as softwoods has been investigated by UV resonance Raman spectroscopy (UVRRS). UVRR spectra showed degradation of aromatic structures accompanied by the formation of both ortho- and para-quinone structures as a result of photodegradation of wood surfaces. There was a rapid decrease in the intensities of all the lignin-associated bands accompanied by broadening of aromatic bands at 1602 cm-1 and in the region of 1500–1000 cm-1 due to formation of unsaturated structures arisen from lignin degradation. The rate of lignin degradation was observed by measuring the relative intensity of aromatic band at 1602 cm-1 during photodegradation. The rates of UV-degradation of hardwood and softwood surfaces were compared and it was found that hardwoods degrade at a faster rate than softwoods. The syringyl structures in hardwood lignin are more sensitive to photochemical degradation than guaiacyl structures. The rate of photodegradation increased with increasing intensity of the irradiated laser energy.

ChemInform Abstract: UV Resonance Raman Examination of the Azurin Tryptophan Environment and Energy Relaxation Pathways

ChemInform ◽  
2010 ◽  
Vol 23 (1) ◽  
pp. no-no
Author(s):  
J. A. SWEENEY ◽  
P. A. HARMON ◽  
S. A. ASHER ◽  
C. M. HUTNIK ◽  
A. G. SZABO
Keyword(s):  
Resonance Raman ◽  
Energy Relaxation ◽  
Uv Resonance Raman
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