Toward a Hydrodynamic Description of Bimolecular Collisions in Micelles. An Experimental Test of the Effect of the Nature of the Quencher on the Fluorescence Quenching of Pyrene in SDS Micelles and in Bulk Liquids

2003 ◽  
Vol 107 (37) ◽  
pp. 10312-10318 ◽  
Author(s):  
Radha Ranganathan ◽  
Carolina Vautier-Giongo ◽  
Barney L. Bales
1981 ◽  
Vol 85 (9) ◽  
pp. 1198-1202 ◽  
Author(s):  
J. C. Dederen ◽  
M. Van der Auweraer ◽  
F. C. De Schryver

2020 ◽  
Vol 19 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Tim Kohlmann ◽  
Martin Goez

The cell and its inmates: time-resolved fluorescence quenching analyzed such as to give meaningful insight into key properties of micelles and intramicellar complexes.


2007 ◽  
Vol 85 (7-8) ◽  
pp. 513-519 ◽  
Author(s):  
Anna Carnini ◽  
Trinh T Nguyen ◽  
David T Cramb

Inhaled anesthetics were introduced in surgery over a century ago. To this day, the molecular mechanism of anesthetic action remains largely unknown. However, ion-channels of neuronal membranes are believed to be the most- likely molecular targets of inhaled anesthetics. In the study presented here, we investigated the interaction of a simplified ion-channel system, gramicidin, with halothane, a small haloalkane inhaled anesthetic in various environments. Fluorescence-quenching experiments of gramicidin D in dioleoylphosphatidylcholine (DOPC) large unilamellar vesicles (LUVS) have shown that halothane can directly interact with the ion channel (KSV = 66 M–1). Halothane quenched the fluorescence from tryptophan residues located at the lipid bilayer – aqueous interfaces as well as those tryptophans located deeper in the bilayer. Quenching data from gramicidin D in sodium dodecyl sulfide (SDS) micelles revealed that the tryptophan residues located at the micelle–solvent interface were preferentially quenched by halothane (KSV = 22 M–1). In 1-octanol, fluorescence quenching was observed, but with a lower KSV value (KSV = 6 M–1) than in DOPC LUVS and SDS micelles. Taken together, these results indicate that halothane interactions with gramicidin, mediated by a lipid bilayer, are the strongest, and that the mechanism of anesthetic action may also be lipid-mediated.


2008 ◽  
Vol 27 (3, Suppl) ◽  
pp. S197-S206 ◽  
Author(s):  
Andrew W. Hertel ◽  
Emily A. Finch ◽  
Kristina M. Kelly ◽  
Christie King ◽  
Harry Lando ◽  
...  

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