“Double Click” Reaction on 7-Deazaguanine DNA: Synthesis and Excimer Fluorescence of Nucleosides and Oligonucleotides with Branched Side Chains Decorated with Proximal Pyrenes

2010 ◽  
Vol 75 (2) ◽  
pp. 284-295 ◽  
Author(s):  
Frank Seela ◽  
Sachin A. Ingale
Keyword(s):  
Dna Synthesis ◽  
Click Reaction ◽  
Side Chains ◽  
Excimer Fluorescence

Preparation of HA Hydrogel by Click Chemistry

2015 ◽  
Vol 1088 ◽  
pp. 286-289
Author(s):  
Yuan Cui ◽  
Jing Peng Zhang ◽  
Wei Zhang ◽  
Qian Duan
Keyword(s):  
Hyaluronic Acid ◽  
Click Chemistry ◽  
Molecular Design ◽  
Click Reaction ◽  
Side Chains ◽  
H Nmr ◽  
Hydrogel Formation

"Click chemistry" was used in this paper to get a novel hyaluronic acid (HA) hydrogel by modified HA molecules' side chains with azide and alkynyl groups to perform "Click" reaction. The HA hydrogel can be achieved "in situ" injection gel. The properties of the HA hydrogel can be controlled by controlling the amount of alkynyl and azide groups on side chains to achieve controllable HA hydrogel by HA molecular design. The reactant, product and hydrogel were characterized by 1H NMR, FTIR and morphology observation to determine the processes of reaction and can hydrogel formation.

DNA with Branched Internal Side Chains: Synthesis of 5-Tripropargylamine-dU and Conjugation by an Azide-Alkyne Double Click Reaction

ChemBioChem ◽  
2008 ◽  
Vol 9 (14) ◽  
pp. 2305-2316 ◽  
Author(s):  
Venkata Ramana Sirivolu ◽  
Padmaja Chittepu ◽  
Frank Seela
Keyword(s):  
Click Reaction ◽  
Side Chains ◽  
Internal Side

scholarly journals Molecular Brushes with a Polyimide Backbone and Poly(ε-Caprolactone) Side Chains by the Combination of ATRP, ROP, and CuAAC

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3312
Author(s):  
Anna V. Kashina ◽  
Tamara K. Meleshko ◽  
Natalia N. Bogorad ◽  
Viktor K. Lavrentyev ◽  
Alexander V. Yakimansky
Keyword(s):  
Molecular Weight ◽  
Click Reaction ◽  
Triazole Ring ◽  
Scattering Data ◽  
Degree Of Crystallinity ◽  
Hydroxyl Group ◽  
Synthesis Methods ◽  
Side Chains ◽  
Molecular Brushes ◽  
Molecular Weight Characteristics

An approach to the synthesis of the novel molecular brushes with a polyimide (PI) backbone and poly(ε-caprolactone) (PCL) side chains was developed. To obtain such copolymers, a combination of various synthesis methods was used, including polycondensation, atom transfer radical polymerization (ATRP), ring opening polymerization (ROP), and Cu (I)-catalyzed azide-alkyne Huisgen cycloaddition (CuAAC). ATRP of 2-hydroxyethyl methacrylate (HEMA) on PI macroinitiator followed by ROP of ε-caprolactone (CL) provided a “brush on brush” structure PI-g-(PHEMA-g-PCL). For the synthesis of PI-g-PCL two synthetic routes combining ROP and CuAAC were compared: (1) polymer-analogous transformations of a multicenter PI macroinitiator with an initiating hydroxyl group separated from the main chain by a triazole ring followed by ROP of CL, or (2) a separate synthesis of macromonomers with the desirable functional groups (polyimide with azide groups and PCL with terminal alkyne groups), followed by a click reaction. Results showed that the first approach allows to obtain graft copolymers with a PI backbone and relatively short PCL side chains. While the implementation of the second approach leads to a more significant increase in the molecular weight, but unreacted linear PCL remains in the system. Obtained macroinitiators and copolymers were characterized using 1H NMR and IR spectroscopy, their molecular weight characteristics were determined by SEC with triple detection. TGA and DSC were used to determine their thermal properties. X-ray scattering data showed that the introduction of a polyimide block into the polycaprolactone matrix did not change the degree of crystallinity of PCL.

Facile synthesis of functional poly(vinylene sulfide)s containing donor–acceptor chromophores by a double click reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (64) ◽  
pp. 59327-59332 ◽  
Author(s):  
Dong Wang ◽  
Qingsen Guo ◽  
Hong Gao ◽  
Zhou Yang ◽  
Hui Cao ◽  
...  
Keyword(s):  
Click Reaction ◽  
Side Chains ◽  
Facile Synthesis ◽  
Click Polymerization ◽  
Donor Acceptor

The new electronically active poly(vinylene sulfide)s containing dialkylaniline-substituted electron-rich alkynes in the side chains were designed and synthesized by the ‘thiol-click’ polymerization.

scholarly journals Nucleoside macrocycles formed by intramolecular click reaction: efficient cyclization of pyrimidine nucleosides decorated with 5'-azido residues and 5-octadiynyl side chains

2018 ◽  
Vol 14 ◽  
pp. 2404-2410 ◽  
Author(s):  
Jiang Liu ◽  
Peter Leonard ◽  
Sebastian L Müller ◽  
Constantin Daniliuc ◽  
Frank Seela
Keyword(s):  
Click Reaction ◽  
Coupling Constants ◽  
Protecting Groups ◽  
Side Chains ◽  
X Ray ◽  
Pyrimidine Nucleosides ◽  
H Nmr ◽  
High Yields ◽  
Very High ◽  
Sugar Conformation

Copper(I)-promoted "click" cyclization in the presence of TBTA afforded nucleoside macrocycles in very high yields (≈70%) without using protecting groups. To this end, dU and dC derivatives functionalized at the 5-position of the nucleobase with octadiynyl side chains and with azido groups at the 5’-position of the sugar moieties were synthesized. The macrocycles display freely accessible Watson–Crick recognition sites. The conformation of the 16-membered macrocycle was deduced from X-ray analysis and 1H,1H-NMR coupling constants. The sugar conformation (N vs S) was different in solution as compared to the solid state.

scholarly journals Dynamics of protein binding to sites of nascent unscheduled DNA repair synthesis in non-proliferating cells

2020 ◽  
Author(s):  
Claudia Scalera ◽  
Ilaria Dutto ◽  
Francesca Barbazza ◽  
Raefa Abou Khouzam ◽  
Giulio Ticli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Click Reaction ◽  
Excision Repair ◽  
Proliferating Cells ◽  
Repair Synthesis ◽  
Dynamic Association ◽  
Dna Repair Synthesis

ABSTRACTThe analysis of DNA repair mechanisms is of fundamental importance to understand how cells remove DNA damage and maintain their genome stability. Investigating the dynamic association of proteins at sites of active DNA synthesis has been successfully performed at DNA replication forks, providing important information on the process, and allowing the identification of new players acting at these sites. However, the applicability of these studies to DNA repair events at sites of nascent unscheduled DNA synthesis (UDS) in non-proliferating cells has been never tested. Here, we describe the analysis of dynamics association of protein participating in nucleotide excision repair (NER), and in other DNA repair processes, at sites of nascent UDS in non-proliferating cells, to avoid interference by DNA replication. Labeling with 5-ethynyl-2’-deoxyuridine (EdU) after DNA damage, followed by click reaction to biotinylate these sites, permits the analysis of dynamic association of proteins, such as DNA polymerases δ and κ, as well as PCNA, to active DNA repair synthesis sites. The suitability of this technique to identify new factors present at active UDS sites is illustrated by two examples of proteins previously unknown to participate in the UV-induced DNA repair process.

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