Efficient Preparation of Aminoxyacyl Amides, Aminoxy Hybrid Peptides, and α-Aminoxy Peptides

2009 ◽  
Vol 74 (22) ◽  
pp. 8690-8694 ◽  
Author(s):  
Alan R. Katritzky ◽  
Ilker Avan ◽  
Srinivasa R. Tala
Keyword(s):  
Hybrid Peptides ◽  
Aminoxy Peptides

scholarly journals Improved Antimicrobial Activities of Synthetic-Hybrid Bacteriocins Designed from Enterocin E50-52 and Pediocin PA-1

2014 ◽  
Vol 81 (5) ◽  
pp. 1661-1667 ◽  
Author(s):  
Santosh Kumar Tiwari ◽  
Katia Sutyak Noll ◽  
Veronica L. Cavera ◽  
Michael L. Chikindas
Keyword(s):  
Micrococcus Luteus ◽  
Electrical Potential ◽  
Gram Negative Bacteria ◽  
Additional Advantage ◽  
Gram Negative ◽  
Content Type ◽  
Intracellular Atp ◽  
Hybrid Peptides ◽  
C Terminus ◽  
N Terminus

ABSTRACTTwo hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bacteriocins showed reduced MICs compared to those of their natural counterparts. The MICs of hybrid PE and EP were 64- and 32-fold lower, respectively, than the MIC of pediocin PA-1 and 8- and 4-fold lower, respectively, than the MIC of enterocin E50-52. In this study, the effect of hybrid as well as wild-type (WT) bacteriocins on the transmembrane electrical potential (ΔΨ) and their ability to induce the efflux of intracellular ATP were investigated. Enterocin E50-52, pediocin PA-1, and hybrid bacteriocin PE were able to dissipate ΔΨ, but EP was unable to deplete this component. Both hybrid bacteriocins caused a loss of the intracellular concentration of ATP. EP, however, caused a faster efflux than PE and enterocin E50-52. Enterocin E50-52 and hybrids PE and EP were active against the Gram-positive and Gram-negative bacteria tested, such asMicrococcus luteus,Salmonella entericaserovar Enteritidis 20E1090, andEscherichia coliO157:H7. The hybrid bacteriocins designed and described herein are antimicrobial peptides with MICs lower those of their natural counterparts. Both hybrid peptides induce the loss of intracellular ATP and are capable of inhibiting Gram-negative bacteria, and PE dissipates the electrical potential. In this study, the MIC of hybrid bacteriocin PE decreased 64-fold compared to the MIC of its natural peptide counterpart, pediocin PA-1. Inhibition of Gram-negative pathogens confers an additional advantage for the application of these peptides in therapeutics.

Hybrid peptides in the landscape of drug discovery

Peptides ◽  
2017 ◽  
Vol 90 ◽  
pp. A1-A2 ◽  
Author(s):  
Daniel Fourmy
Keyword(s):  
Drug Discovery ◽  
Hybrid Peptides

scholarly journals Biochemical and pharmacological investigation of novel nociceptin/OFQ analogues and N/OFQ-RYYRIK hybrid peptides

Peptides ◽  
2019 ◽  
Vol 112 ◽  
pp. 106-113 ◽  
Author(s):  
Anna I. Erdei ◽  
Adina Borbély ◽  
Anna Magyar ◽  
Edina Szűcs ◽  
Ferenc Ötvös ◽  
...  
Keyword(s):  
Pharmacological Investigation ◽  
Hybrid Peptides

Potent Antimicrobial Activity of Lipidated Short α,γ-Hybrid Peptides

ChemMedChem ◽  
2017 ◽  
Vol 12 (19) ◽  
pp. 1610-1615
Author(s):  
Sushil N. Benke ◽  
Hirekodathakallu V. Thulasiram ◽  
Hosahudya N. Gopi
Keyword(s):  
Antimicrobial Activity ◽  
Hybrid Peptides

Synthesis and Conformational Studies of γ−Aminoxy Peptides

2008 ◽  
Vol 130 (2) ◽  
pp. 743-755 ◽  
Author(s):  
Fei Chen ◽  
Ke-Sheng Song ◽  
Yun-Dong Wu ◽  
Dan Yang
Keyword(s):  
Conformational Studies ◽  
Aminoxy Peptides

scholarly journals Multiple Antimicrobial Effects of Hybrid Peptides Synthesized Based on the Sequence of Ribosomal S1 Protein from Staphylococcus aureus

2022 ◽  
Vol 23 (1) ◽  
pp. 524
Author(s):  
Sergey V. Kravchenko ◽  
Pavel A. Domnin ◽  
Sergei Y. Grishin ◽  
Alexander V. Panfilov ◽  
Viacheslav N. Azev ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptides ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Cell Penetrating Peptide ◽  
Gram Negative Bacteria ◽  
Antimicrobial Effects ◽  
Bacterial Proteins ◽  
Hybrid Peptides ◽  
Successful Approach

The need to develop new antimicrobial peptides is due to the high resistance of pathogenic bacteria to traditional antibiotics now and in the future. The creation of synthetic peptide constructs is a common and successful approach to the development of new antimicrobial peptides. In this work, we use a simple, flexible, and scalable technique to create hybrid antimicrobial peptides containing amyloidogenic regions of the ribosomal S1 protein from Staphylococcus aureus. While the cell-penetrating peptide allows the peptide to enter the bacterial cell, the amyloidogenic site provides an antimicrobial effect by coaggregating with functional bacterial proteins. We have demonstrated the antimicrobial effects of the R23F, R23DI, and R23EI hybrid peptides against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus. R23F, R23DI, and R23EI can be used as antimicrobial peptides against Gram-positive and Gram-negative bacteria resistant to traditional antibiotics.

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