Potent Antimicrobial Activity of Lipidated Short α,γ-Hybrid Peptides

ChemMedChem ◽  
2017 ◽  
Vol 12 (19) ◽  
pp. 1610-1615
Author(s):  
Sushil N. Benke ◽  
Hirekodathakallu V. Thulasiram ◽  
Hosahudya N. Gopi
Keyword(s):  
Antimicrobial Activity ◽  
Hybrid Peptides

scholarly journals Cecropin a - magainin 2 hybrid peptides having potent antimicrobial activity with low hemolytic effect

IUBMB Life ◽  
1998 ◽  
Vol 44 (6) ◽  
pp. 1119-1126 ◽  
Author(s):  
Song Yub Shin ◽  
Joo Hyun Kang ◽  
Myung Kyu Lee ◽  
Sun Young Kim ◽  
Yangmee Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Hybrid Peptides ◽  
Cecropin A ◽  
Hemolytic Effect

scholarly journals Rational Designed Hybrid Peptides Show up to a 6-fold Increase in Antimicrobial Activity and Demonstrate Different Ultrastructural Changes as the Parental Peptides measured by BioSAXS

2021 ◽  
Author(s):  
Kai Hilpert ◽  
Jurnorain Gani ◽  
Christoph Rumancev ◽  
Nathan Simpson ◽  
Paula Matilde Lopez-Perez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Fold Increase ◽  
Ultrastructural Changes ◽  
Therapeutic Window ◽  
Resistant Bacteria ◽  
Haemolytic Activity ◽  
Hybrid Peptide ◽  
E Coli ◽  
Hybrid Peptides

Antimicrobial peptides (AMPs) are a promising class of compounds being developed against multi-drug resistant bacteria. Hybridization has been reported to increase antimicrobial activity. Here, two proline-rich peptides (consP1: VRKPPYLPRPRPRPL-CONH2 and Bac5-v291: RWRRPIRRRPIRPPFWR-CONH2) were combined with two arginine-isoleucine-rich peptides (optP1: KIILRIRWR-CONH2 and optP7: KRRVRWIIW-CONH2). Proline-rich antimicrobial peptides (PrAMPs) are known to inhibit the bacterial ribosome, shown also for Bac5-v291, whereas it is hypothesized a dirty drug model for the arginine-isoleucine-rich peptides. That hypothesis was underpinned by transmission electron microscopy and biological small-angle X-ray scattering (BioSAXS). The hybrid peptides showed a stronger antimicrobial activity compared to the proline-rich peptides, except when compared to Bac5-v291 against E. coli. The increase in activity compared to the arginine-isoleucine-rich peptides was up to 6-fold, however, it was not a general increase but was dependent on the combination of peptides and bacteria. BioSAXS experiments revealed that proline-rich peptides and arginine-isoleucine-rich peptides induce very different ultrastructural changes in E. coli, whereas a hybrid peptide (hyP7B5GK) shows changes, different to both parental peptides and the untreated control. These different ultrastructural changes indicated that the mode of action of the parental peptides is different from each other as well as from the hybrid peptide hyP7B5GK. All peptides showed very low haemolytic activity, some of them showed a 100-fold or larger therapeutic window, demonstrating the potential for further drug development.

scholarly journals Rational Designed Hybrid Peptides Show up to a 6-Fold Increase in Antimicrobial Activity and Demonstrate Different Ultrastructural Changes as the Parental Peptides Measured by BioSAXS

2021 ◽  
Vol 12 ◽  
Author(s):  
Kai Hilpert ◽  
Jurnorain Gani ◽  
Christoph Rumancev ◽  
Nathan Simpson ◽  
Paula Matilde Lopez-Perez ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Fold Increase ◽  
Ultrastructural Changes ◽  
Therapeutic Window ◽  
Resistant Bacteria ◽  
Haemolytic Activity ◽  
Hybrid Peptide ◽  
E Coli ◽  
Hybrid Peptides

Antimicrobial peptides (AMPs) are a promising class of compounds being developed against multi-drug resistant bacteria. Hybridization has been reported to increase antimicrobial activity. Here, two proline-rich peptides (consP1: VRKPPYLPRPRPRPL-CONH2 and Bac5-v291: RWRRPIRRRPIRPPFWR-CONH2) were combined with two arginine-isoleucine-rich peptides (optP1: KIILRIRWR-CONH2 and optP7: KRRVRWIIW-CONH2). Proline-rich antimicrobial peptides (PrAMPs) are known to inhibit the bacterial ribosome, shown also for Bac5-v291, whereas it is hypothesized a “dirty drug” model for the arginine-isoleucine-rich peptides. That hypothesis was underpinned by transmission electron microscopy and biological small-angle X-ray scattering (BioSAXS). The strength of BioSAXS is the power to detect ultrastructural changes in millions of cells in a short time (seconds) in a high-throughput manner. This information can be used to classify antimicrobial compounds into groups according to the ultrastructural changes they inflict on bacteria and how the bacteria react towards that assault. Based on previous studies, this correlates very well with different modes of action. Due to the novelty of this approach direct identification of the target of the antimicrobial compound is not yet fully established, more research is needed. More research is needed to address this limitation. The hybrid peptides showed a stronger antimicrobial activity compared to the proline-rich peptides, except when compared to Bac5-v291 against E. coli. The increase in activity compared to the arginine-isoleucine-rich peptides was up to 6-fold, however, it was not a general increase but was dependent on the combination of peptides and bacteria. BioSAXS experiments revealed that proline-rich peptides and arginine-isoleucine-rich peptides induce very different ultrastructural changes in E. coli, whereas a hybrid peptide (hyP7B5GK) shows changes, different to both parental peptides and the untreated control. These different ultrastructural changes indicated that the mode of action of the parental peptides might be different from each other as well as from the hybrid peptide hyP7B5GK. All peptides showed very low haemolytic activity, some of them showed a 100-fold or larger therapeutic window, demonstrating the potential for further drug development.

Antimicrobial activity of withanolides from the leaves of Withania aristata

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
L Araujo ◽  
N Padilla ◽  
GG Llanos ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antimicrobial Activity

Antimicrobial activity of Eurycoma longifolia and Momordica charantia

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
R Łos ◽  
K Skalicka-Wozniak ◽  
J Widelski ◽  
A Malm ◽  
K Głowniak
Keyword(s):  
Antimicrobial Activity ◽  
Momordica Charantia ◽  
Eurycoma Longifolia

Antioxidant and antimicrobial activity of willow herb (Epilobium angustifolium L.)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak
Keyword(s):  
Antimicrobial Activity ◽  
Epilobium Angustifolium ◽  
Antioxidant And Antimicrobial Activity
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