A facile biomimetic method for oxidative deamination of primary amines to aldehydes via transposition of an imine functionality

1981 ◽  
Vol 46 (9) ◽  
pp. 1937-1938 ◽  
Author(s):  
James H. Babler ◽  
Benedict J. Invergo
Keyword(s):  
Primary Amines ◽  
Oxidative Deamination ◽  
Biomimetic Method

Oxidative deamination of sec-alkyl primary amines with 3,5-di-tert-butyl-1,2-benzoquinone; a second look

1988 ◽  
Vol 53 (26) ◽  
pp. 5994-5998 ◽  
Author(s):  
Robert F. X. Klein ◽  
Lisa M. Bargas ◽  
Vaclav Horak
Keyword(s):  
Primary Amines ◽  
Oxidative Deamination ◽  
Tert Butyl ◽  
Second Look

Oxidative Deamination of Various Primary Amines to the Corresponding Carbonyl Compounds by UsingN-tert-Butylphenylsulfinimidoyl Chloride

2001 ◽  
Vol 30 (7) ◽  
pp. 712-713 ◽  
Author(s):  
Jun-ichi Matsuo ◽  
Asahi Kawana ◽  
Yoshio Fukuda ◽  
Teruaki Mukaiyama
Keyword(s):  
Carbonyl Compounds ◽  
Primary Amines ◽  
Oxidative Deamination

scholarly journals Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Ryo Kubota ◽  
Michael J. Reid ◽  
Kuo Lee Lieu ◽  
Mark Orme ◽  
Christine Diamond ◽  
...  
Keyword(s):  
Amine Oxidase ◽  
Leukocyte Adhesion ◽  
Primary Amines ◽  
Oxidative Deamination ◽  
Adhesion Protein ◽  
Common Marker ◽  
Small Primary ◽  
Vascular Adhesion ◽  
Vascular Adhesion Protein 1

Vascular adhesion protein-1 (VAP-1) is an ectoenzyme that functions as a copper-containing amine oxidase and is involved in leukocyte adhesion at sites of inflammation. Inhibition of VAP-1 oxidative deamination has become an attractive target for anti-inflammatory therapy with demonstrated efficacy in rodent models of inflammation. A previous comparison of purified recombinant VAP-1 from mouse, rat, monkey, and human gene sequences predicted that rodent VAP-1 would have higher affinity for smaller hydrophilic substrates/inhibitors because of its narrower and more hydrophilic active site channel. An optimized in vitro oxidative deamination fluorescence assay with benzylamine (BA) was used to compare inhibition of five known inhibitors in recombinant mouse, rat, and human VAP-1. Human VAP-1 was more sensitive compared to rat or mouse VAP-1 (lowest IC50 concentration) to semicarbazide but was least sensitive to hydralazine and LJP-1207. Hydralazine had a lower IC50 in rats compared to humans, although not significant. However, the IC50 of hydralazine was significantly higher in the rat compared to mouse VAP-1. The larger hydrophobic compounds from Astellas (compound 35c) and Boehringer Ingelheim (PXS-4728A) were hypothesized to have higher binding affinity for human VAP-1 compared to rodent VAP-1 since the channel in human VAP-1 is larger and more hydrophobic than that in rodent VAP-1. Although the sensitivity of these two inhibitors was the lowest in the mouse enzyme, we found no significant differences between mouse, rat, and human VAP-1. Michaelis-Menten kinetics of the small primary amines phenylethylamine and tyramine were also compared to the common marker substrate BA demonstrating that BA had the highest affinity among the substrates. Rat VAP-1 had the highest affinity for all three substrates and mouse VAP-1 had intermediate affinity for BA and phenylethylamine, but tyramine was not a substrate for mouse VAP-1 under these assay conditions. These results suggest that comparing oxidative deamination in mouse and rat VAP-1 may be important if using these species for preclinical efficacy models.

Sign in / Sign up

Export Citation Format

Share Document