Template-Directed Crosslinking of Oligonucleotides: Site-Specific Covalent Modification of dG-N7 within Duplex DNA

1995 ◽  
Vol 60 (20) ◽  
pp. 6252-6253 ◽  
Author(s):  
Robert S. Coleman ◽  
Edward A. Kesicki
Keyword(s):  
Covalent Modification ◽  
Duplex Dna ◽  
Site Specific

scholarly journals Effects of site-specific substitution of 5-fluorouridine on the stabilities of duplex DNA and RNA

1995 ◽  
Vol 23 (19) ◽  
pp. 3916-3921 ◽  
Author(s):  
Parag V. Sahasrabudhe ◽  
Richard T. Pon ◽  
William H. Gmeiner
Keyword(s):  
Duplex Dna ◽  
Site Specific ◽  
Dna And Rna

Site-Specific Covalent Modification of RNA Guided by Functionality-Transfer Oligodeoxynucleotides

2009 ◽  
Vol 20 (4) ◽  
pp. 799-803 ◽  
Author(s):  
Kazumitsu Onizuka ◽  
Yosuke Taniguchi ◽  
Shigeki Sasaki
Keyword(s):  
Covalent Modification ◽  
Site Specific

scholarly journals Site-Specific Recombination of TemperateMyxococcus xanthus Phage Mx8: Regulation of Integrase Activity by Reversible, Covalent Modification

1999 ◽  
Vol 181 (13) ◽  
pp. 4062-4070 ◽  
Author(s):  
Vincent Magrini ◽  
Michael L. Storms ◽  
Philip Youderian
Keyword(s):  
Specific Activity ◽  
Active Form ◽  
Attachment Site ◽  
Covalent Modification ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Site Specific Integration ◽  
C Terminus ◽  
Primary Amino ◽  
Reversible Covalent

ABSTRACT Temperate Myxococcus xanthus phage Mx8 integrates into the attB locus of the M. xanthus genome. The phage attachment site, attP, is required in cisfor integration and lies within the int (integrase) coding sequence. Site-specific integration of Mx8 alters the 3′ end ofint to generate the modified intX gene, which encodes a less active form of integrase with a different C terminus. The phage-encoded (Int) form of integrase promotes attP × attB recombination more efficiently than attR × attB, attL × attB, or attB × attB recombination. The attP and attBsites share a common core. Sequences flanking both sides of theattP core within the int gene are necessary forattP function. This information shows that the directionality of the integration reaction depends on arm sequences flanking both sides of the attP core. Expression of theuoi gene immediately upstream of int inhibits integrative (attP × attB) recombination, supporting the idea that uoi encodes the Mx8 excisionase. Integrase catalyzes a reaction that alters the primary sequence of its gene; the change in the primary amino acid sequence of Mx8 integrase resulting from the reaction that it catalyzes is a novel mechanism by which the reversible, covalent modification of an enzyme is used to regulate its specific activity. The lower specific activity of the prophage-encoded IntX integrase acts to limit excisive site-specific recombination in lysogens carrying a single Mx8 prophage, which are less immune to superinfection than lysogens carrying multiple, tandem prophages. Thus, this mechanism serves to regulate Mx8 site-specific recombination and superinfection immunity coordinately and thereby to preserve the integrity of the lysogenic state.

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