Site-specific cleavage of duplex DNA with a .lambda. repressor-staphylococcal nuclease hybrid

1990 ◽  
Vol 112 (11) ◽  
pp. 4579-4580 ◽  
Author(s):  
Dehua Pei ◽  
Peter G. Schultz
Keyword(s):  
Staphylococcal Nuclease ◽  
Duplex Dna ◽  
Lambda Repressor ◽  
Site Specific

scholarly journals Effects of site-specific substitution of 5-fluorouridine on the stabilities of duplex DNA and RNA

1995 ◽  
Vol 23 (19) ◽  
pp. 3916-3921 ◽  
Author(s):  
Parag V. Sahasrabudhe ◽  
Richard T. Pon ◽  
William H. Gmeiner
Keyword(s):  
Duplex Dna ◽  
Site Specific ◽  
Dna And Rna

scholarly journals The genetic control of the immune response to staphylococcal nuclease VI. Recombination between genes determining the A/J anti-nuclease idiotypes and the heavy chain allotype locus.

1977 ◽  
Vol 146 (6) ◽  
pp. 1603-1612 ◽  
Author(s):  
D S Pisetsky ◽  
D H Sachs
Keyword(s):  
Immune Response ◽  
Heavy Chain ◽  
Genetic Linkage ◽  
Germ Line ◽  
Variable Region ◽  
Staphylococcal Nuclease ◽  
Progeny Testing ◽  
Structural Genes ◽  
Site Specific ◽  
Very High

Rat antisera detecting binding site-specific idiotypic determinants on anti-nuclease antibodies from A/J mice have been used to define the A/J anti-nuclease idiotype and to investigate its genetic linkage as a variable region marker. Analysis of the segregation of the A/J idiotype in progeny of the backcross (B10.A X A/J) X B10.A showed linkage of the idiotype to the Ig-1e heavy chain allotype locus. There was, however, a very high apparent frequency of recombination, with 7 of 101 backcross animals having a recombinant phenotype. All of these putative recombinants were accounted for by Ig-1b/Ig-1b homozygotes which bore the A/J idiotype, and none by Ig-1b/Ig-1e heterozygotes lacking the idiotype. On progeny testing of these animals in another backcross to B10.A the recombinant trait bred true. If this idiotype is indeed a marker for variable region structural genes, then the germ line gene pool must be very large or there must be special genetic mechanism to account for the increased recombinational frequency observed.

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