Synthesis and Characterization of Environment-Sensitive Fluorescent Ligands for Human 5-HT1AReceptors with 1-Arylpiperazine Structure†

2009 ◽  
Vol 52 (23) ◽  
pp. 7892-7896 ◽  
Author(s):  
Enza Lacivita ◽  
Marcello Leopoldo ◽  
Anna Carmela Masotti ◽  
Carmela Inglese ◽  
Francesco Berardi ◽  
...  
Keyword(s):  
Synthesis And Characterization ◽  
Fluorescent Ligands

Synthesis and Characterization of Selective Fluorescent Ligands for the neurokinin NK2 Receptor

1994 ◽  
Vol 37 (13) ◽  
pp. 1991-1995 ◽  
Author(s):  
Charles G. Bradshaw ◽  
Karin Ceszkowski ◽  
Gerardo Turcatti ◽  
Isabel J. M. Beresford ◽  
Andre Chollet
Keyword(s):  
Synthesis And Characterization ◽  
Fluorescent Ligands ◽  
Nk2 Receptor

scholarly journals Synthesis and Characterization of High-Affinity 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene-Labeled Fluorescent Ligands for Human β-Adrenoceptors

2011 ◽  
Vol 54 (19) ◽  
pp. 6874-6887 ◽  
Author(s):  
Jillian G. Baker ◽  
Luke A. Adams ◽  
Karolina Salchow ◽  
Shailesh N. Mistry ◽  
Richard J. Middleton ◽  
...  
Keyword(s):  
Synthesis And Characterization ◽  
High Affinity ◽  
Fluorescent Ligands

scholarly journals Environment-Sensitive Fluorescence of 7-Nitrobenz-2-oxa-1,3-Diazol-4-yl (NBD)-Labeled Ligands for Serotonin Receptors

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3848
Author(s):  
Parijat Sarkar ◽  
Kaleeckal G. Harikumar ◽  
Satinder S. Rawat ◽  
Sanjib Das ◽  
Tushar K. Chakraborty ◽  
...  
Keyword(s):  
Cell Surface ◽  
Serotonin Receptors ◽  
Multiple Roles ◽  
Synthesis And Characterization ◽  
Future Studies ◽  
Serotonin1a Receptor ◽  
Red Edge ◽  
Red Edge Excitation Shift ◽  
Fluorescent Ligands

Serotonin is a neurotransmitter that plays a crucial role in the regulation of several behavioral and cognitive functions by binding to a number of different serotonin receptors present on the cell surface. We report here the synthesis and characterization of several novel fluorescent analogs of serotonin in which the fluorescent NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) group is covalently attached to serotonin. The fluorescent ligands compete with the serotonin1A receptor specific radiolabeled agonist for binding to the receptor. Interestingly, these fluorescent ligands display a high environmental sensitivity of their fluorescence. Importantly, the human serotonin1A receptor stably expressed in CHO-K1 cells could be specifically labeled with one of the fluorescent ligands with minimal nonspecific labeling. Interestingly, we show by spectral imaging that the NBD-labeled ligand exhibits a red edge excitation shift (REES) of 29 nm when bound to the receptor, implying that it is localized in a restricted microenvironment. Taken together, our results show that NBD-labeled serotonin analogs offer an attractive fluorescent approach for elucidating the molecular environment of the serotonin binding site in serotonin receptors. In view of the multiple roles played by the serotonergic systems in the central and peripheral nervous systems, these fluorescent ligands would be useful in future studies involving serotonin receptors.

Synthesis and Characterization of 4,4-Difluoro-4-bora-3a,4a-Diaza-s-Indacene (BODIPY)-Labeled Fluorescent Ligands for the Mu Opioid Receptor

1997 ◽  
Vol 54 (12) ◽  
pp. 1315-1322 ◽  
Author(s):  
Paul J Emmerson ◽  
Sydney Archer ◽  
Wageeh El-Hamouly ◽  
Alfred Mansour ◽  
Huda Akil ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Mu Opioid Receptor ◽  
Synthesis And Characterization ◽  
Mu Opioid ◽  
Fluorescent Ligands

Synthesis and Characterization of the Three Dicyclopentapyrenes

1996 ◽  
Vol 61 (10) ◽  
pp. 3572-3572
Author(s):  
Lawrence T. Scott ◽  
Atena Necula
Keyword(s):  
Synthesis And Characterization

Synthesis and characterization of TiN / Ti / AISI 410 coatings

2018 ◽  
Vol 2 (1) ◽  
pp. 7
Author(s):  
S Chirino ◽  
Jaime Diaz ◽  
N Monteblanco ◽  
E Valderrama
Keyword(s):  
Synthesis And Characterization ◽  
Photoemission Spectroscopy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Columnar Grains ◽  
Tin Thin Films ◽  
Steel Aisi ◽  
Interacting Surfaces ◽  
Stainless Steel Aisi

The synthesis and characterization of Ti and TiN thin films of different thicknesses was carried out on a martensitic stainless steel AISI 410 substrate used for tool manufacturing. The mechanical parameters between the interacting surfaces such as thickness, adhesion and hardness were measured. By means of the scanning electron microscope (SEM) the superficial morphology of the Ti/TiN interface was observed, finding that the growth was of columnar grains and by means of EDAX the existence of titanium was verified.  Using X-ray diffraction (XRD) it was possible to observe the presence of residual stresses (~ -3.1 GPa) due to the different crystalline phases in the coating. Under X-ray photoemission spectroscopy (XPS) it was possible to observe the molecular chemical composition of the coating surface, being Ti-N, Ti-N-O and Ti-O the predominant ones.

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