Additions and Corrections - Synthesis and Anabolic Activity of 3-Methylene and 3-Methylsteroids of the Androstane and 19-Norandrostane Series.

1964 ◽  
Vol 7 (6) ◽  
pp. 835-835
Author(s):  
K. Irmscher ◽  
H.-G. Kraft ◽  
K. Brückner
Keyword(s):  
Anabolic Activity

STUDY OF ANABOLIC ACTIVITY OF DRY EXTRACTS OF LEAVES AND ROOTSTALKS OF IRIS HUNGARICA IN INTACT ANIMALS

Fitoterapia ◽  
2020 ◽  
Vol 2 (2) ◽  
pp. 50-55
Author(s):  
G. F. Kerimova ◽  
◽  
V. A. Rybak ◽  
А. V. Krechun ◽  
V. М. Kovalev ◽  
...  
Keyword(s):  
Anabolic Activity

scholarly journals In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ralee Spooner ◽  
Kris M. Weigel ◽  
Peter L. Harrison ◽  
KyuLim Lee ◽  
Gerard A. Cangelosi ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Periodontal Pathogens ◽  
Anabolic Activity ◽  
Filifactor Alocis

The catabolic function of the α-aminoadipic acid pathway in plants is associated with unidirectional activity of lysine–oxoglutarate reductase, but not saccharopine dehydrogenase

2000 ◽  
Vol 351 (1) ◽  
pp. 215-220
Author(s):  
Xiaohong ZHU ◽  
Guiliang TANG ◽  
Gad GALILI
Keyword(s):  
Adipic Acid ◽  
Expression System ◽  
Yeast Cells ◽  
Deletion Mutants ◽  
Null Mutant ◽  
Anabolic Activity ◽  
Saccharopine Dehydrogenase ◽  
Intermediate Metabolites ◽  
Acid Pathway ◽  
Transcriptional Controls

Whereas plants and animals use the α-aminoadipic acid pathway to catabolize lysine, yeast and fungi use the very same pathway to synthesize lysine. These two groups of organisms also possess structurally distinct forms of two enzymes in this pathway, namely lysine–oxoglutarate reductase (lysine–ketoglutarate reductase; LKR) and saccharopine dehydrogenase (SDH): in plants and animals these enzymes are linked on to a single bifunctional polypeptide, while in yeast and fungi they exist as separate entities. In addition, yeast LKR and SDH possess bi-directional activities, and their anabolic function is regulated by complex transcriptional and post-transcriptional controls, which apparently ascertain differential accumulation of intermediate metabolites; in plants, the regulation of the catabolic function of these two enzymes is not known. To elucidate the regulation of the catabolic function of plant bifunctional LKR/SDH enzymes, we have used yeast as an expression system to test whether a plant LKR/SDH also possesses bi-directional LKR and SDH activities, similar to the yeast enzymes. The Arabidopsis enzyme complemented a yeast SDH, but not LKR, null mutant. Identical results were obtained when deletion mutants encoding only the LKR or SDH domains of this bifunctional polypeptide were expressed individually in the yeast cells. Moreover, activity assays showed that the Arabidopsis LKR possessed catabolic, but not anabolic, activity, and its uni-directional activity stems from its structure rather than its linkage to SDH. Our results suggest that the uni-directional activity of LKR plays an important role in regulating the catabolic function of the α-amino adipic acid pathway in plants.

scholarly journals Spatially-resolved correlative microscopy and microbial identification reveals dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment

2020 ◽  
Author(s):  
Jeffrey Marlow ◽  
Rachel Spietz ◽  
Keun-Young Kim ◽  
Mark Ellisman ◽  
Peter Girguis ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Microbial Communities ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Community Members ◽  
Marsh Sediment ◽  
Anabolic Activity ◽  
Salt Marsh Sediment ◽  
Rrna Gene Sequencing ◽  
And Function

AbstractCoastal salt marshes are key sites of biogeochemical cycling and ideal systems in which to investigate the community structure of complex microbial communities. Here, we clarify structural-functional relationships among microorganisms and their mineralogical environment, revealing previously undescribed metabolic activity patterns and precise spatial arrangements within salt marsh sediment. Following 3.7-day in situ incubations with a non-canonical amino acid that was incorporated into new biomass, samples were embedded and analyzed by correlative fluorescence and electron microscopy to map the microscale arrangements of anabolically active and inactive organisms alongside mineral grains. Parallel sediment samples were examined by fluorescence-activated cell sorting and 16S rRNA gene sequencing to link anabolic activity to taxonomic identity. Both approaches demonstrated a rapid decline in the proportion of anabolically active cells with depth into salt marsh sediment, from ∼60% in the top cm to 10-25% between 2-7 cm. From the top to the bottom, the most prominent active community members shifted from sulfur cycling phototrophic consortia, to sulfate-reducing bacteria likely oxidizing organic compounds, to fermentative lineages. Correlative microscopy revealed more abundant (and more anabolically active) organisms around non-quartz minerals including rutile, orthoclase, and plagioclase. Microbe-mineral relationships appear to be dynamic and context-dependent arbiters of biogeochemical cycling.Statement of SignificanceMicroscale spatial relationships dictate critical aspects of a microbiome’s inner workings and emergent properties, such as evolutionary pathways, niche development, and community structure and function. However, many commonly used methods in microbial ecology neglect this parameter – obscuring important microbe-microbe and microbe-mineral interactions – and instead employ bulk-scale methodologies that are incapable of resolving these intricate relationships.This benchmark study presents a compelling new approach for exploring the anabolic activity of a complex microbial community by mapping the precise spatial configuration of anabolically active organisms within mineralogically heterogeneous sediment through in situ incubation, resin embedding, and correlative fluorescence and electron microscopy. In parallel, active organisms were identified through fluorescence-activated cell sorting and 16S rRNA gene sequencing, enabling a powerful interpretive framework connecting location, identity, activity, and putative biogeochemical roles of microbial community members.We deploy this novel approach in salt marsh sediment, revealing quantitative insights into the fundamental principles that govern the structure and function of sediment-hosted microbial communities. In particular, at different sediment horizons, we observed striking changes in the proportion of anabolically active cells, the identities of the most prominent active community members, and the nature of microbe-mineral affiliations. Improved approaches for understanding microscale ecosystems in a new light, such as those presented here, reveal environmental parameters that promote or constrain metabolic activity and clarify the impact that microbial communities have on our world.

scholarly journals Ketosteroid standardized Cissus quadrangularis L. extract and its anabolic activity: Time to look beyond ketosteroid?

2016 ◽  
Vol 12 (46) ◽  
pp. 213 ◽  
Author(s):  
AtulN Jadhav ◽  
Mohammed Rafiq ◽  
Rajendran Devanathan ◽  
Mohammed Azeemuddin ◽  
SuryakanthD Anturlikar ◽  
...  
Keyword(s):  
Activity Time ◽  
Anabolic Activity ◽  
Cissus Quadrangularis

RS-66271: Molecular design and in vivo bone anabolic activity

Peptides 1994 ◽  
1995 ◽  
pp. 133-134
Author(s):  
J. L. Krstenansky ◽  
T. L. Ho ◽  
Z. Avnur ◽  
D. Leaffer ◽  
J. P. Caulfield ◽  
...  
Keyword(s):  
Molecular Design ◽  
Anabolic Activity

Ketosteroid standardized Cissus quadrangularis L. extract and its anabolic activity: Time to look beyond ketosteroid?

2016 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
AtulN Jadhav ◽  
Mohammed Rafiq ◽  
Rajendran Devanathan ◽  
Mohammed Azeemuddin ◽  
SuryakanthD Anturlikar ◽  
...  
Keyword(s):  
Activity Time ◽  
Anabolic Activity ◽  
Cissus Quadrangularis

Targeting Bortezomib to Bone Increases Its Bone Anabolic Activity and Reduces Systemic Adverse Effects in Mice

2019 ◽  
Vol 35 (2) ◽  
pp. 343-356 ◽  
Author(s):  
Hua Wang ◽  
Hengwei Zhang ◽  
Venkat Srinivasan ◽  
Jianguo Tao ◽  
Wen Sun ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Anabolic Activity
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