Direct analysis of carbofuran and two nonconjugated metabolites in crops by high-pressure liquid chromatography with UV absorption detection

1977 ◽  
Vol 25 (6) ◽  
pp. 1362-1365 ◽  
Author(s):  
James F. Lawrence ◽  
Raymonde Leduc
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Direct Analysis ◽  
Uv Absorption

Identification of Some Benzodiazepines of Forensic Interest

1979 ◽  
Vol 62 (4) ◽  
pp. 799-807
Author(s):  
F Taylor Noggle ◽  
C Randall Clark
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ir Spectra ◽  
High Pressure Liquid Chromatography ◽  
Dosage Form ◽  
Uv Absorption ◽  
Hplc Separation ◽  
Absorption Data ◽  
Solid Dosage ◽  
Ir Spectrophotometry

Abstract Ten benzodiazepines–clorazepate, nitrazepam, clonazepam, oxazepam, lorazepam, chlordiazepoxide, N-desmethyldiazepam, diazepam, prazepam, and flurazepam–in solid dosage form are identified by ultraviolet (UY) and infrared (IR) spectrophotometry and by high pressure liquid chromatography (HPLC). UV absorption data and IR spectra of the 10 benzodiazepines are provided and HPLC separation methods are described.

Sensitive and selective assay for adenosine using high-pressure liquid chromatography with fluorometry

1983 ◽  
Vol 245 (5) ◽  
pp. H887-H890 ◽  
Author(s):  
M. K. Jacobson ◽  
L. M. Hemingway ◽  
T. A. Farrell ◽  
C. E. Jones
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Direct Detection ◽  
Peak Height ◽  
Uv Absorption ◽  
New Method ◽  
Fluorometric Method ◽  
Primary Advantage ◽  
Fluorescence Peak

A new method for quantitation of adenosine was tested in canine myocardial extracts. The method involves incubation of the extract with chloroacetaldehyde to form the fluorescing adenosine derivative 1, N6-ethenoadenosine. The ethenoadenosine is separated by high-pressure liquid chromatography (HPLC) and quantitated by fluorometry. Experiments demonstrated that 1) the method is selective for adenosine, 2) fluorescence peak height is linearly related to the quantity of ethenoadenosine, and 3) adenosine in the extract is quantitatively converted to ethenoadenosine by the incubation procedure. Also, within the range of adenosine concentrations seen in five extracts, estimates of myocardial adenosine content with the fluorometric method were nearly identical to those using the more routine technique of HPLC with direct detection by ultraviolet (UV) absorption. A primary advantage of the fluorometric method is its greater sensitivity. As little as 0.50 pmol on the column could be quantitated by fluorescence, compared with approximately 20 pmol with UV absorption. Because of the greater sensitivity, the fluorometric method should be more easily applied to samples with smaller adenosine concentrations.

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