Metabolism of 2,4-dichlorophenoxyacetic acid by wheat cell suspension cultures

1977 ◽  
Vol 25 (6) ◽  
pp. 1308-1314 ◽  
Author(s):  
Douglas W. Bristol ◽  
Ahmed Murad Ghanuni ◽  
Arland E. Oleson
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

Effect of either 2,4-dichlorophenoxyacetic acid or kinetin on production and composition of various fractions from extracellular polysaccharides produced by cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (15) ◽  
pp. 1816-1822 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Extracellular Polysaccharides ◽  
Dichlorophenoxyacetic Acid ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
2,4 Dichlorophenoxyacetic Acid

Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.

In vitro culture of Bouvardia ternifolia

1982 ◽  
Vol 60 (6) ◽  
pp. 917-921 ◽  
Author(s):  
Leonor Fernandez ◽  
Estela Sanchez de Jimenez
Keyword(s):  
Indoleacetic Acid ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Napthaleneacetic Acid ◽  
Leaf Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

Callus cultures were induced from radicle and leaf tissues of Bouvardia ternifolia (trompetilla). Optimum growth regulator concentrations for radicle callus cultures were 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.005 mg/L kinetin; for leaf callus they were either 2 mg/L naphthaleneacetic acid and 0.002 mg/L benzylaminopurine or 5 mg/L of idoleacetic acid and 0.01 mg/L kinetin. Callus has been maintained in culture for nearly 3 years with a very rapid growth rate.A generation time of approximately 24 to 28 h was obtained for batch cell suspension cultures. Production of protoplasts from suspension cultures was optimized with a yield of 70 to 90%. Protoplast culture was achieved in droplets of fresh medium with 2 mg/L napthaleneacetic acid, 0.01 mg/L benzylaminopurine, and0.5 M mannitol. After 2 years, callus in culture still retained its organogenetic capacity. An average of 18 complete plantlets from approximately 2 g of callus can be obtained after transfer to medium with 0.1 mg/L indoleacetic acid and 0.1 mg/L benzylaminopurine.

Somatic embryogenesis and plant regeneration from cell suspension derived protoplasts of Solanum melongena (eggplant)

1986 ◽  
Vol 64 (2) ◽  
pp. 355-361 ◽  
Author(s):  
S. Gleddie ◽  
W. A. Keller ◽  
G. Setterfield
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Suspension ◽  
Somatic Embryos ◽  
Solanum Melongena ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Subsequent Regeneration

Cell suspension cultures of eggplant (Solanum melongena L.) were initiated from embryogenic callus cultures and maintained in medium supplemented with either 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Higher yields of protoplasts were obtained from cells grown in 2,4-D than in NAA. The efficiency of cell division was also greater in protoplast cultures derived from cells grown in the presence of 2,4-D. Protoplast-derived cells formed somatic embryos in modified Kao or Nagata and Takebe media which were supplemented with 1 mg/L 2,4-D. Early stages of embryogenesis were observed in liquid medium; however, these embryos and associated cell colonies were transferred onto agar-solidified medium for subsequent regeneration. Mature plants were established in soil in the greenhouse.

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