Decreased aniline hydroxylase activity in Japanese quail due to dietary DDT

1972 ◽  
Vol 20 (3) ◽  
pp. 553-557 ◽  
Author(s):  
Jerry L. Sell ◽  
Kenneth L. Davison ◽  
Kockanda B. Poonacha
Keyword(s):  
Japanese Quail ◽  
Hydroxylase Activity ◽  
Aniline Hydroxylase

Medicinal herb, Thonningia sanguinea protects against aflatoxin B1 acute hepatotoxicity in Fischer 344 rats

1998 ◽  
Vol 17 (8) ◽  
pp. 418-423 ◽  
Author(s):  
Maxwell Afari Gyamfi ◽  
Yoko Aniya
Keyword(s):  
Liver Injury ◽  
Aflatoxin B1 ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Fischer 344 Rats ◽  
Hydroxylase Activity ◽  
Aniline Hydroxylase ◽  
Fischer 344 ◽  
Cytochrome P450 Activity ◽  
Thonningia Sanguinea

1. Thonningia sanguinea, a plant used prophylactically against bronchial asthma in Ghana was recently found to have antioxidative and hepatoprotective actions in our laboratory. 2. In this study, the effect of T. sanguinea extract on certain biochemical indices in serum and liver of Fischer 344 rats given a single intraperitoneal (i.p.) dose (1 mg/kg) of aflatoxin B1 (AFB1) was investigated. 3. Administration of AFB1 resulted in significant increases in serum alanine aminotransferase (ALT) and glutathione S-transferase (GST) levels and a signifi-cant decrease in aniline hydroxylase activity in liver microsomes. When T. sanguinea (5 ml/kg) was intraperitoneally administered to rats 12 h and 1 h before AFB1, liver injury was significantly reduced as seen in the decreased levels of serum ALT and serum GST. However, the decrease in aniline hydroxylase activity by AFB1 was not recovered but enhanced by T. sanguinea pre-treatment. 4. Kinetic analysis of cytochrome P450 activity of rat liver microsomes in vitro demonstrated that T. sanguinea inhibited aniline hydroxylase non-competitively suggesting depression of biotransformation of AFB1 to toxic metabolites. 5. The data indicate a hepatoprotective action of T. sanguinea against AFB1-induced liver injury.

Modulation of Microsomal Cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in Rat Liver

1996 ◽  
Vol 24 (01) ◽  
pp. 19-29 ◽  
Author(s):  
Jaw-Jou Kang ◽  
Yi-Chien Chen ◽  
Wei-Chung Kuo ◽  
Terri Chen ◽  
Yu-Wen Cheng ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Medicinal Herbs ◽  
Hydroxylase Activity ◽  
Microsomal Cytochrome ◽  
Aniline Hydroxylase ◽  
Scutellariae Radix ◽  
Microsomal Cytochrome P450 ◽  
Pyrene Hydroxylase

The present study has determined the effects of Scutellariae Radix (Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.

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