Aniline-Hydroxylase Activity of Myoglobin by Coupling with a Membrane-Bound Electron Transport System

1995 ◽  
Vol 24 (8) ◽  
pp. 657-658 ◽  
Author(s):  
Itaru Hamachi ◽  
Akio Fujita ◽  
Toyoki Kunitake
Keyword(s):  
Electron Transport ◽  
Transport System ◽  
Electron Transport System ◽  
Hydroxylase Activity ◽  
Aniline Hydroxylase ◽  
Membrane Bound ◽  
Bound Electron

Electroimmunodiffusion — a Powerful Tool for Quantitative Determinations of Both Soluble and Membrane Bound Chloroplast ATPase, CF1

1983 ◽  
Vol 38 (9-10) ◽  
pp. 799-805 ◽  
Author(s):  
Peter Roos ◽  
Richard J. Berzborn
Keyword(s):  
Electron Transport ◽  
Atp Synthase ◽  
Transport System ◽  
Simultaneous Measurement ◽  
Electron Transport System ◽  
Limiting Factor ◽  
Monospecific Antiserum ◽  
Membrane Bound ◽  
Turn Over ◽  
Over Time

1. Electroimmunodiffusion can be used for routine specific quantitative microdeterminations of photosynthetic ATP-synthase. The method has been worked out for samples of both the dissociated (CF1) and the membrane bound enzyme (CF0CF1).2. Only a monospecific antiserum against CF1 , is required for relative determinations of CF1 in any fraction. If beyond this an absolute determination is intended of either the separated or the membrane bound ATPase, in addition a pure CF1 , is needed for calibration.3. A varying CF1/chlorophyll ratio between 1/700 and 1/1200 has been found for thylakoids of spinach plants grown under various light intensities.4. Since the amount of CF1 , was the limiting factor of photophosphorylation capacity in the electron transport system used (PMS), from simultaneous measurement of CF1 , content and photophosphorylation capacity a turn over time of the ATPsynthase of 4 ms /ATP /CF0CF1 could be recalculated.

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