Determination of Cystine as Cysteic Acid after Low Voltage Paper Electrophoresis

1966 ◽  
Vol 14 (5) ◽  
pp. 496-498 ◽  
Author(s):  
Omer Pelletier
Keyword(s):  
Low Voltage ◽  
Paper Electrophoresis ◽  
Cysteic Acid

scholarly journals Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin

2002 ◽  
Vol 67 (8-9) ◽  
pp. 573-579 ◽  
Author(s):  
Divna Djokic ◽  
Drina Jankovic ◽  
Tatjana Maksin
Keyword(s):  
Radiochemical Purity ◽  
Low Voltage ◽  
Paper Electrophoresis ◽  
Human Albumin ◽  
Gel Chromatography ◽  
Labelling Efficiency ◽  
Column Method ◽  
Macroaggregated Albumin ◽  
99Mtc Labelling

Anew procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman No 1 and ITLC-SAstrips. Asystem of two solvents (acetone and 1MNaCl or 0.9%NaCl) was used for 3MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5%) and percent radiochemical impurities of 99mTc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 ?350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.

A Simple Low-Voltage Paper Electrophoretic Method for the Determination of Urinary Vanillylmandelic Acid (VMA)

1963 ◽  
Vol 9 (5) ◽  
pp. 615-619 ◽  
Author(s):  
F Eichhorn ◽  
A Rutenberg
Keyword(s):  
Amino Acids ◽  
Mandelic Acid ◽  
Low Voltage ◽  
Accurate Method ◽  
Paper Electrophoresis ◽  
Vanillylmandelic Acid ◽  
Electrophoretic Method ◽  
Vanillyl Mandelic Acid ◽  
Normal Urine

Abstract A simple and accurate method for the determination of urinary 3-methoxy-4-hydroxy-mandelic acid, vanillyl-mandelic acid (VMA), is described. The separation of VMA from all other phenolic acids in approximately 0.05 ml. untreated urine is achieved by using low-voltage paper electrophoresis with different buffer concentrations for the cathode and the anode. The concentration of the electrophoretically separated VMA is determined colorimetrically in the eluate. Values of excreted VMA in 24-hr. normal urine and those from subjects with pheochromocytoma differ considerably. We are now applying this new principle of "two solutions paper electrophoresis" for better separation of amino acids, proteins, and other biologic mixtures.

Identification of N-Monoacetylcystine in Uraemic Plasma

1981 ◽  
Vol 60 (3) ◽  
pp. 331-334 ◽  
Author(s):  
F. Gejyo ◽  
G. Ito ◽  
Y. Kinoshita
Keyword(s):  
Plasma Levels ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Exchange Chromatography ◽  
Cysteic Acid ◽  
Homocysteic Acid ◽  
Normal Limits ◽  
Acidic Nature ◽  
Sulphur Amino Acids

1. An unidentified ninhydrin-positive substance of an acidic nature was detected in the plasma of uraemic patients. This substance was isolated from haemodialysate by ion-exchange chromatography and gel filtration, and identified as a sulphur-containing amino acid: N-monoacetylcystine. 2. The quantitative determination of sulphur amino acids in plasma revealed that the plasma levels of cysteic acid, homocysteic acid, taurine, cystine and cystathionine as well as N-monoacetylcystine in uraemic patients were markedly higher than in normal subjects (P < 0.001 for each). However, the plasma levels of methionine in uraemic patients were within normal limits.

scholarly journals Determination of cysteic acid in animal tissues by gas chromatography.

1986 ◽  
Vol 35 (4) ◽  
pp. 389-393
Author(s):  
Hiroyuki KATAOKA ◽  
Kazuyuki OHISHI ◽  
Noriko INOUE ◽  
Masami MAKITA
Keyword(s):  
Gas Chromatography ◽  
Cysteic Acid ◽  
Animal Tissues

scholarly journals Calculations, norming and reducing of electrical energy losses in city electrical networks

2019 ◽  
Vol 21 (3) ◽  
pp. 46-54
Author(s):  
A. V. Lykin ◽  
E. A. Utkin
Keyword(s):  
High Voltage ◽  
Power Transmission ◽  
Low Voltage ◽  
Demand Curve ◽  
Electrical Energy ◽  
Electrical Network ◽  
Energy Losses ◽  
Capital Investments ◽  
Electrical Networks

The article considers the feasibility of changing the structure of a distribution electrical network by transferring points of electricity transformation as close to consumers as possible. This approach is based on installation of pole-mounted transformer substations (PMTS) near consumer groups and changes the topology of the electrical network. At the same time, for groups of consumers, the configuration of sections of the low-voltage network, including service drops, changes. The efficiency of approaching transformer substations to consumers was estimated by the reduction in electrical energy losses due to the expansion of the high-voltage network. The calculation of electrical losses was carried out according to twenty-four hour consumer demand curve. To estimate the power losses in each section of the electrical network of high and low voltage, the calculated expressions were obtained. For the considered example, the electrical energy losses in the whole network with a modified topology is reduced by about two times, while in a high-voltage network with the same transmitted power, the losses are reduced to a practically insignificant level, and in installed PMTS transformers they increase mainly due to the rise in total idle losses. The payback period of additional capital investments in option with modified topology will be significantly greater if payback is assessed only by saving losses cost. Consequently, the determination of the feasibility of applying this approach should be carried out taking into account such factors as increasing the reliability of electricity supply, improving the quality of electricity, and increasing the power transmission capacity of the main part of electrical network.

Determination of the future actuator demand of adaptive smart low voltage grids

2017 ◽  
Author(s):  
Philippe Steinbusch ◽  
Jan Meese ◽  
Roman Uhlig ◽  
Jan Mehlich ◽  
Marcus Stotzel ◽  
...  
Keyword(s):  
Low Voltage ◽  
The Future

Identification of Human Hemoglobins

1957 ◽  
Vol 3 (1) ◽  
pp. 1-19 ◽  
Author(s):  
C A J Goldberg
Keyword(s):  
Paper Electrophoresis ◽  
Electrophoretic Separations ◽  
Freely Suspended ◽  
Physical And Chemical

Abstract Practical physical and chemical procedures available for the identification of hemoglobins have been discussed. These include electrophoresis, chromatography, alkali denaturation, and ferrohemoglobin solubility. The conditions essential for satisfactory electrophoretic separations have been considered and two procedures for paper electrophoresis at pH 6.5, 7.8, and 8.6 using apparatus for compressed paper strips and for freely suspended horizontal strips are presented. A method for the determination of ferrohemoglobin solubility also has been described.

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