Characterization of Lipids and Lignans in Brewer’s Spent Grain and Its Enzymatically Extracted Fraction

2012 ◽  
Vol 60 (39) ◽  
pp. 9910-9917 ◽  
Author(s):  
Piritta Niemi ◽  
Tarja Tamminen ◽  
Annika Smeds ◽  
Kaarina Viljanen ◽  
Taina Ohra-aho ◽  
...  
Keyword(s):  
Brewer’S Spent Grain ◽  
Spent Grain

Preparation and characterization of brewer’s spent grain protein-chitosan composite films

2015 ◽  
Vol 52 (11) ◽  
pp. 7549-7555 ◽  
Author(s):  
Ji-Hyeon Lee ◽  
Ji-Hyun Lee ◽  
Hyun-Ju Yang ◽  
Kyung Bin Song
Keyword(s):  
Composite Films ◽  
Grain Protein ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Chitosan Composite

scholarly journals Production, Purification, and Characterization of a MajorPenicillium glabrumXylanase Using Brewer's Spent Grain as Substrate

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Adriana Knob ◽  
Susan Michelz Beitel ◽  
Diana Fortkamp ◽  
César Rafael Fanchini Terrasan ◽  
Alex Fernando de Almeida
Keyword(s):  
Xylanase Activity ◽  
Stationary Condition ◽  
Purification And Characterization ◽  
Xylanase Production ◽  
Brewer’S Spent Grain ◽  
Agroindustrial Waste ◽  
Sds Page ◽  
Exclusion Chromatography ◽  
Spent Grain

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production byPenicillium glabrumusing brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained whenP. glabrumwas grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase fromP. glabrumwas purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+and the reducing agentsβ-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

scholarly journals EFFECT OF REACTION TIME AND SODIUM HYDROXIDE CONCENTRATION ON DELIGNIFICATION AND ENZYMATIC HYDROLYSISOF BREWER’S SPENT GRAIN FROM WO BRAZILIAN BREWERS

2021 ◽  
Vol 55 (1-2) ◽  
pp. 101-112
Author(s):  
CARLA LOBO GOMES ◽  
ELIZABETH GONÇALVES ◽  
CARLOS ALBERTO GALEANO SUAREZ ◽  
DASCIANA DE SOUSA RODRIGUES ◽  
INTI CAVALCANTI MONTANO
Keyword(s):  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Factorial Design ◽  
Contact Time ◽  
Point Contact ◽  
Sodium Hydroxide Concentration ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Hydrolysis Of

Brazil, being one of the main beer producers, generates brewer’s spent grain as a main by-product of this industry, which is mainly composed of cellulose, hemicelluloses, lignin and extractives. The alkaline pretreatment of brewer’s spent grain received from two breweries is studied in this work, namely breweries Imperial (B1) and Colombina (B2). Factorial design (22) was realized with three experiments at the central point: contact time (30, 60, 90 min) and NaOH concentration (4%, 6%, 8%). It was found that the presence of extractives causes interference in the characterization of the material. The delignification process allowed obtaining materials with lower lignin percentages when higher NaOH concentrations were used, reaching percentages of lignin loss with values between 85-95%, in both materials, but for these conditions, the losses of cellulose were considerable – of 35-43%. Enzymatic hydrolysis of the pretreated materials achieved conversions greater than 70%, emphasizing that the greatest conversions were obtained with material B1, where almost the whole cellulose was hydrolyzed.

scholarly journals Biosurfactant from endophytic Bacillus pumilus 2A: physicochemical characterization, production and optimization and potential for plant growth promotion

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Olga Marchut-Mikołajczyk ◽  
Piotr Drożdżyński ◽  
Arkadiusz Polewczyk ◽  
Wojciech Smułek ◽  
Tadeusz Antczak
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Plant Growth ◽  
Taguchi Method ◽  
Beta Vulgaris ◽  
Food Processing ◽  
Bacillus Pumilus ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
By Products

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

scholarly journals Potential of Brewer’s Spent Grain as a Potential Replacement of Wood in pMDI, UF or MUF Bonded Particleboard

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 319
Author(s):  
Marius Cătălin Barbu ◽  
Zeno Montecuccoli ◽  
Jakob Förg ◽  
Ulrike Barbeck ◽  
Petr Klímek ◽  
...  
Keyword(s):  
Internal Bond ◽  
Mechanical Performance ◽  
Animal Feed ◽  
Urea Formaldehyde ◽  
Modulus Of Rupture ◽  
Brewer’S Spent Grain ◽  
Beer Brewing ◽  
Removal Of Heavy Metals ◽  
Dry Conditions ◽  
Spent Grain

Brewer’s spent grain (BSG) is the richest by-product (85%) of the beer-brewing industry, that can be upcycled in a plentiful of applications, from animal feed, bioethanol production or for removal of heavy metals from wastewater. The aim of this research is to investigate the mechanical, physical and structural properties of particleboard manufactured with a mixture of wood particles and BSG gradually added/replacement in 10%, 30% and 50%, glued with polymeric diisocyanate (pMDI), urea-formaldehyde (UF) and melamine urea-formaldehyde (MUF) adhesives. The density, internal bond, modulus of rupture, modulus of elasticity, screw withdrawal resistance, thickness swelling and water absorption were tested. Furthermore, scanning electron microscopy anaylsis was carried out to analyze the structure of the panels after the internal bond test. Overall, it was shown that the adding of BSG decreases the mechanical performance of particleboard, due to reduction of the bonding between wood and BSG particles. This decrease has been associated with the structural differences proven by SEM inspection. Interaction of particles with the adhesive is different for boards containing BSG compared to those made from wood. Nevertheless, decrease in the mechanical properties was not critical for particleboards produced with 10% BSG which could be potentially classified as a P2 type, this means application in non-load-bearing panel for interior use in dry conditions, with high dimensional stability and stiffness.

scholarly journals Recycling of Brewer’s Spent Grain as a Biosorbent by Nitro-Oxidation for Uranyl Ion Removal from Wastewater

ACS Omega ◽  
2021 ◽  
Author(s):  
Yi Su ◽  
Marco Wenzel ◽  
Silvia Paasch ◽  
Markus Seifert ◽  
Wendelin Böhm ◽  
...  
Keyword(s):  
Uranyl Ion ◽  
Brewer’S Spent Grain ◽  
Ion Removal ◽  
Spent Grain

scholarly journals Anticholinesterase Activities of Different Solvent Extracts of Brewer’s Spent Grain

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 930
Author(s):  
Rares I. Birsan ◽  
Peter Wilde ◽  
Keith W. Waldron ◽  
Dilip K. Rai
Keyword(s):  
Ethyl Acetate Fraction ◽  
Therapeutic Approaches ◽  
Total Polyphenol ◽  
Brewer’S Spent Grain ◽  
Dietary Polyphenols ◽  
Synthetic Drugs ◽  
Naturally Occurring ◽  
Diferulic Acid ◽  
Spent Grain ◽  
Butyryl Cholinesterase

Cholinesterases, involved in acetylcholine catabolism in the central and peripheral nervous system, have been strongly linked with neurodegenerative diseases. Current therapeutic approaches using synthetic drugs present several side effects. Hence, there is an increasing research interest in naturally-occurring dietary polyphenols, which are also considered efficacious. Food processing by-products such as brewer’s spent grain (BSG) would be a potential bio-source of polyphenols. In this study, polyphenol-rich BSG extracts using 60% acetone and 0.75% NaOH solutions were generated, which were further subjected to liquid–liquid partitioning using various organic solvents. The water-partitioned fractions of the saponified extracts had the highest total polyphenol content (6.2 ± 2.8mgGAE/g dw) as determined by Folin–Ciocalteu reagent, while the LC-MS/MS showed ethyl acetate fraction with the highest phenolics (2.9 ± 0.3mg/g BSG dw). The best inhibitions of acetyl- (37.9 ± 2.9%) and butyryl- (53.6 ± 7.7%) cholinesterases were shown by the diethyl ether fraction of the saponified extract. This fraction contained the highest sum of quantified phenolics (99 ± 21.2µg/mg of extract), and with significant (p < 0.01) inhibitory contribution of decarboxylated-diferulic acid. Amongst the standards, caffeic acid presented the highest inhibition for both cholinesterases, 25.5 ± 0.2% for acetyl- and 52.3 ± 0.8% for butyryl-cholinesterase, respectively, whilst the blends insignificantly inhibited both cholinesterases. The results showed that polyphenol-rich BSG fractions have potentials as natural anti-cholinesterase agents.

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