scholarly journals A New Approach on Brewer’s Spent Grains Treatment and Potential Use as Lignocellulosic Yeast Cells Carriers

2012 ◽  
Vol 60 (23) ◽  
pp. 5994-5999 ◽  
Author(s):  
Eduardo J. Pires ◽  
Héctor A. Ruiz ◽  
José A. Teixeira ◽  
António A. Vicente
2019 ◽  
Vol 15 ◽  
pp. 40-47 ◽  
Author(s):  
Ly Ly Pham ◽  
Thomas Y. Sheffield ◽  
Prachi Pradeep ◽  
Jason Brown ◽  
Derik E. Haggard ◽  
...  

2003 ◽  
Vol 96 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Catarina Almeida ◽  
Tomáš Brányik ◽  
Pedro Moradas-Ferreira ◽  
José Teixeira

1987 ◽  
Vol 165 (4) ◽  
pp. 1160-1171 ◽  
Author(s):  
P J Barr ◽  
H L Gibson ◽  
V Enea ◽  
D E Arnot ◽  
M R Hollingdale ◽  
...  

DNA coding for 234 amino acids of the circumsporozoite (CS) protein of Plasmodium vivax was incorporated into yeast expression vectors. The DNA encoded all the repeat domain and codons for a highly conserved sequence, KLKQP, found in CS proteins from all malaria parasites. Yeast cells transformed with these autonomously replicating plasmids expressed, upon induction, high levels of the CS polypeptide. The malaria antigen was purified in good yields from yeast extracts and was injected into mice using alum as adjuvant. The antibodies recognized the authentic CS protein, and at high dilutions, they inhibited the invasion of hepatocytes by sporozoites in vitro.


2021 ◽  
Vol 7 (2) ◽  
pp. 31-36
Author(s):  
Amanda Sousa Monteiro ◽  
Denise Dantas ◽  
Takashi Yojo

In the last fifteen years, a new approach has emerged in the design field: the process of development of new materials and the involvement of designers as original creators, making use of underutilized local materials. This paper aims to describe the preparation of an Amazonian palm tree fiber (Manicaria saccifera Gaertn.) for the use at composites materials. The raw materials were extracted from the Marajó archipelago - Brazil by local communities that traditionally work with the material. The process involves the measure of weight, length, and thickness at six different points of the raw specimen; manual washing and cleaning; drying with stopping criterion; and visual analyses of the specimen with trimming and cutting, for the potential use at composites. The results for 80 specimens show that the method adopted allowed easy and quick preparation of the specimens and can be used for other local materials, respecting its characteristics.


2020 ◽  
Vol 13 (2) ◽  
pp. 532-551
Author(s):  
Krzysztof Bierwiaczonek ◽  
Grzegorz Gawron ◽  
Robert Pyka ◽  
Małgorzata Suchacka

Metropolises are currently regarded as nodes in a global network of flows, becoming the driving forces for territorial development, characterised by high potential of creativity and the innovations generated as a result. A contemporary challenge is to find adequate concepts for analysing and understanding metropolitan innovativeness. The objective of the article is to provide the necessary features by which a new approach to studying metropolitan inventiveness can be adopted. The formulation of this model was inspired by the independently functioning concepts of metropolisation, innovativeness, and the concepts of place. By considering these three perspectives together and their specific methods of analysis, it is possible to describe and explain the process of generating innovations in contemporary metropolises. This article is divided into three main sections illustrating how the concepts of innovation environments, networks, and districts combined with the dynamic concept of place, all relate to innovation place. The analysis presented serves as the basis for the model of an innovation place, it being essential to future initiatives in this sphere of study. The article concludes with methodological remarks indicating the potential use for “innovation space” model.


Author(s):  
Helena Martins ◽  
Artemisa Dores

Gamification is a relatively new approach that allows the use of videogame design techniques in contexts that are originally not game related, including for the promotion and education of health outcomes. Gamification has been used in many contexts, but healthcare practices, which include often boring, frustrating, or painful tasks, can especially benefit from the fun enjoyable games people play for entertainment purposes. Games can be helpful both promoting an increase in health knowledge and behaviors, as well as the positive emotions elicited by health-related contents and behaviors. This chapter begins by discussing the concept of gamification, the gamification toolbox, and gamer taxonomies and the different uses of gamification and game-based approaches in the healthcare context are explored, to figure out what the key success elements are and why this promising approach has yet to achieve its wide-spread potential use.


2019 ◽  
Vol 61 (3) ◽  
pp. 576-583 ◽  
Author(s):  
Raul Sanchez-Mu�oz ◽  
Lorena Almagro ◽  
Rosa M Cusido ◽  
Mercedes Bonfill ◽  
Javier Palazon ◽  
...  

Abstract Taxane diterpenes are secondary metabolites with an important pharmacological role in the treatment of cancer. Taxus spp. biofactories have been used for taxane production, but the lack of knowledge about the taxane biosynthetic pathway and its molecular regulation hinders their optimal function. The difficulties in introducing foreign genes in Taxus spp. genomes hinder the study of the molecular mechanisms involved in taxane production, and a new approach is required to overcome them. In this study, a reliable, simple and fast method to obtain Taxus � media protoplasts was developed, allowing their manipulation in downstream assays for the study of physiological changes in Taxus spp. cells. Using this method, Taxus protoplasts were transiently transfected for the first time, corroborating their suitability for transfection assays and the study of specific physiological responses. The two assayed transcription factors (BIS2 and TSAR2) had a positive effect on the expression of several taxane-related genes, suggesting their potential use for the improvement of taxane yields. Furthermore, the results indicate that the developed method is suitable for obtaining T. � media protoplasts for transfection with the aim of unraveling regulatory mechanisms in taxane production.


Life ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 28 ◽  
Author(s):  
Marta Filipa Simões ◽  
Cristiane Angélica Ottoni ◽  
André Antunes

Metal nanoparticles (MNPs) have been extensively studied. They can be produced via different methods (physical, chemical, or biogenic), but biogenic synthesis has become more relevant, mainly for being referred by many as eco-friendly and more advantageous than others. Biogenic MNPs have been largely used in a wide variety of applications, from industry, to agriculture, to health sectors, among others. Even though they are increasingly researched and used, there is still space for exploring further applications and increasing their functionality and our understanding of their synthesis process. Here, we provide an overview of MNPs and biogenic MNPs, and we analyze the potential application of their formation process to astrobiology and the detection of life on Mars and other worlds. According to current knowledge, we suggest that they can be used as potential biosignatures in extra-terrestrial samples. We present the advantages and disadvantages of this approach, suggest further research, and propose its potential use for the search for life in future space exploration.


2021 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Annemarijn Steijlen ◽  
Jeroen Bastemeijer ◽  
Robbert Nederhoff ◽  
Kaspar Jansen ◽  
Paddy French ◽  
...  

Ammonium levels in sweat can potentially be used to measure muscle fatigue and to diagnose particular metabolic myopathies. To research the potential use of ammonia in sweat as a biomarker, a new real-time monitoring system is developed. This system consists of a capsule that is placed on the skin and ventilated with dry air. A metal-oxide gas sensor in the capsule detects the ammonia that is evaporated from sweat. The sensor system was built, and calibration experiments were performed. The sensors show good sensitivity from 27 mV/ppm to 1.1 mV/ppm in the desired measurement range of 1 to 30 ppm, respectively. A temperature and humidity sensor is integrated to compensate for temperature and humidity effects on the NH3 sensor.


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