Universal Primer-Multiplex-Polymerase Chain Reaction (UP-M-PCR) and Capillary Electrophoresis–Laser-Induced Fluorescence Analysis for the Simultaneous Detection of Six Genetically Modified Maize Lines

2011 ◽  
Vol 59 (10) ◽  
pp. 5188-5194 ◽  
Author(s):  
Chunjiao Zhang ◽  
Wentao Xu ◽  
Zhifang Zhai ◽  
Yunbo Luo ◽  
Xinghua Yan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Capillary Electrophoresis ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Fluorescence Analysis ◽  
Laser Induced Fluorescence ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Universal Primer ◽  
Polymerase Chain

scholarly journals A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xu-long Wu ◽  
Lu Xiao ◽  
Hua Lin ◽  
Miao Yang ◽  
Shi-jie Chen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Capillary Electrophoresis ◽  
Detection Limit ◽  
High Throughput ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Reaction System ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain

Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR) system for the simultaneous detection of nine pathogens in swine. Nine pairs of specific primers and a set of universal primers were designed; the multiplex PCR was established. The specificity and cross-reactivity of this assay were examined, and the detection limit was determined using serial 10-fold dilutions of plasmids containing the target sequences. The assay was further tested using 144 clinical samples. We found that the nine specific amplification peaks were observed, and the assay had a high degree of specificity, without nonspecific amplification. The simultaneous detection limit for the nine viruses reached 10000 copies μL−1 when all of the premixed viral targets were present. Seventy-seven of the clinical samples tested positive for at least one of the viruses; the principal viral infections in the clinical samples were porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus. This approach has much potential for further development of high-throughput detection tools for the diagnosis of diseases in animals.

scholarly journals Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

2016 ◽  
Vol 32 (6) ◽  
pp. 575-579 ◽  
Author(s):  
In Jeong Kang ◽  
Mi-Hyung Kang ◽  
Tae-Hwan Noh ◽  
Hyeong Kwon Shim ◽  
Dong Bum Shin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Polymerase Chain
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