scholarly journals Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction

2005 ◽  
Vol 6 (3) ◽  
pp. 231 ◽  
Author(s):  
Dong Kyun Suh ◽  
Jae Chan Song
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Lawsonia Intracellularis ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Brachyspira Hyodysenteriae ◽  
Polymerase Chain

scholarly journals Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

2016 ◽  
Vol 32 (6) ◽  
pp. 575-579 ◽  
Author(s):  
In Jeong Kang ◽  
Mi-Hyung Kang ◽  
Tae-Hwan Noh ◽  
Hyeong Kwon Shim ◽  
Dong Bum Shin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Development of a Multiplex Polymerase Chain Reaction Method for Simultaneous Detection of Four Cimicifuga Species

Crop Science ◽  
2010 ◽  
Vol 50 (5) ◽  
pp. 1961-1966 ◽  
Author(s):  
Baigalmaa Jigden ◽  
Hongtao Wang ◽  
Yeon-Ju Kim ◽  
Jong-Hun Noh ◽  
Choijamts Gotov ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Combination of Immunomagnetic Separation and Polymerase Chain Reaction for the Simultaneous Detection of Listeria monocytogenes and Salmonella spp. in Food Samples

2001 ◽  
Vol 64 (11) ◽  
pp. 1744-1750 ◽  
Author(s):  
HSIEN-YEE HSIH ◽  
HAU-YANG TSEN
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Simultaneous Detection ◽  
Meat Products ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Pcr Method ◽  
Polymerase Chain

A method that combined the immunomagnetic separation (IMS) technique and the multiplex polymerase chain reaction (PCR) method (i.e., the IMS-mPCR method) was developed for simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. When only the multiplex PCR method was used, it was found that if cell numbers of each of the two target organisms (L. monocytogenes and Salmonella spp.) were above the detection limit, but differed by more than 2 logs—e.g., n × 107 to n × 104 or n × 106 to n × 103—the organism presenting the lower numbers might go undetected. Following the enrichment step with universal preenrichment (UP) broth, if an IMS method using equal quantities of anti-Listeria and anti-Salmonella immunomagnetic beads was performed prior to PCR, both pathogens could be detected unambiguously. Such results could be obtained for target organisms in food samples, such as milk, dairy, and meat products, if similar enrichment and IMS steps were performed prior to PCR.

Sign in / Sign up

Export Citation Format

Share Document