Multiplexed Protein Quantification in Maize Leaves by Liquid Chromatography Coupled with Tandem Mass Spectrometry: An Alternative Tool to Immunoassays for Target Protein Analysis in Genetically Engineered Crops

2011 ◽  
Vol 59 (8) ◽  
pp. 3551-3558 ◽  
Author(s):  
X. Tiger Hu ◽  
Michaela A. Owens
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Protein Analysis ◽  
Genetically Engineered ◽  
Protein Quantification ◽  
Target Protein ◽  
Tandem Mass ◽  
Maize Leaves ◽  
Genetically Engineered Crops

Quantification of intractable membrane proteins in genetically engineered crops by liquid chromatography coupled with tandem mass spectrometry

2017 ◽  
Vol 9 (19) ◽  
pp. 2821-2829 ◽  
Author(s):  
Lindsey J. Schacherer ◽  
Michaela A. Owens ◽  
Tiger X. Hu
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Tandem Mass Spectrometry ◽  
Genetically Engineered ◽  
Soybean Seeds ◽  
Tandem Mass ◽  
Maize Leaves ◽  
Genetically Engineered Crops

Liquid chromatography with tandem mass spectrometry (LC-MS/MS) methods to quantify a membrane protein in genetically engineered maize leaves and another in soybean seeds were developed and validated.

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