Quantitative Determination of Multiple Elements in Botanicals and Dietary Supplements Using ICP-MS

2010 ◽  
Vol 58 (16) ◽  
pp. 8887-8894 ◽  
Author(s):  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Troy J. Smillie ◽  
Nurdan S. Duzgoren-Aydin ◽  
Ikhlas A. Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Icp Ms

scholarly journals Quantitative Determination of the Surface Distribution of Supported Metal Nanoparticles: A Laser Ablation–ICP–MS Based Approach

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 77
Author(s):  
Davide Spanu ◽  
Gilberto Binda ◽  
Marcello Marelli ◽  
Laura Rampazzi ◽  
Sandro Recchia ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Laser Ablation ◽  
Quantitative Determination ◽  
Metal Nanoparticles ◽  
Total Mass ◽  
Functional Materials ◽  
Metal Extraction ◽  
Ms Analysis ◽  
Icp Ms

A laser ablation–inductively coupled plasma–mass spectrometry (LA–ICP–MS) based method is proposed for the quantitative determination of the spatial distribution of metal nanoparticles (NPs) supported on planar substrates. The surface is sampled using tailored ablation patterns and the data are used to define three-dimensional functions describing the spatial distribution of NPs. The volume integrals of such interpolated surfaces are calibrated to obtain the mass distribution of Ag NPs by correlation with the total mass of metal as determined by metal extraction and ICP–MS analysis. Once this mass calibration is carried out on a sacrificial sample, quantifications can be performed over multiple samples by a simple micro-destructive LA–ICP–MS analysis without requiring the extraction/dissolution of metal NPs. The proposed approach is here tested using a model sample consisting of a low-density polyethylene (LDPE) disk decorated with silver NPs, achieving high spatial resolution over cm2-sized samples and very high sensitivity. The developed method is accordingly a useful analytical tool for applications requiring both the total mass and the spatial distribution of metal NPs to be determined without damaging the sample surface (e.g., composite functional materials and NPs, decorated catalysts or electrodic materials).

scholarly journals Development and Validation of an UHPLC Method for Ostarine Determination in Dietary Supplements

2019 ◽  
Vol 65 (2) ◽  
pp. 49-54
Author(s):  
Amalia Miklos ◽  
Amelia Tero-Vescan ◽  
Lénárd Farczádi ◽  
Daniela-Lucia Muntean
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Low Cost ◽  
Reversed Phase ◽  
European Pharmacopoeia ◽  
Average Mass ◽  
7Th Edition ◽  
Development And Validation ◽  
Mg Content

AbstractObjective: The purpose of this study was to develop a low-cost, yet sensitive and precise UHPLC method for the quantitative determination of ostarine from dietary supplements (DS) for athletes. The analytical performance of the method was verified on a DS legally acquired from a specialized website for athletes. The uniformity of mass and content of the ostarine DS was also verified.Methods: For the quantitative determination of ostarine a UHPLC method was developed and validated. The separation was performed using a reversed-phase C18 column, using a mixture of 75% methanol: 25% formic acid 0.1% in isocratic elution, at a flow rate of 0.5 ml/min. The uniformity of mass and content of DS was performed following the methodology described in the European Pharmacopoeia 7th Edition.Results: The validated method was specific and linear on the concentration range of 1-25 µg/ml and was precise and accurate at all concentration levels, according to the official guidelines for validating analytical methods. An average mass of 510 mg content was obtained for the ostarine capsules, with an RSD of 2.41%. Regarding the uniformity of the content, an average of 4.65 mg ostarine/capsule was obtained with an RSD of 1.05%.Conclusions: The developed UHPLC method was suitable, rapid, sensitive and allowed quantitative determination of active substance content in a DS with ostarine (92.91% ostarine/capsule from 5 mg ostarine/capsule declared by the manufacturer).

scholarly journals Quantitative Determination of Pregnanes from Aerial Parts of Caralluma Species Using HPLC-UV and Identification by LC-ESI-TOF

2011 ◽  
Vol 94 (5) ◽  
pp. 1383-1390
Author(s):  
Bharathi Avula ◽  
Yatin J Shukla ◽  
Yan-Hong Wang ◽  
Ikhlas A Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Plant Materials ◽  
Aerial Parts ◽  
Photodiode Array ◽  
Array Detection ◽  
Na Ions

Abstract An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1–5 and 3–15 μg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LC/MS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.

scholarly journals Quantitative Determination of Vinpocetine in Dietary Supplements

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100
Author(s):  
John M. T. French ◽  
Matthew D. King ◽  
Owen M. McDougal
Keyword(s):  
United States ◽  
Quantitative Determination ◽  
Dietary Supplements ◽  
Nootropic Drugs ◽  
Regulatory Policies ◽  
Short And Long Term ◽  
Pharmacologically Active ◽  
Active Substances

Current United States regulatory policies allow for the addition of pharmacologically active substances in dietary supplements if derived from a botanical source. The inclusion of certain nootropic drugs, such as vinpocetine, in dietary supplements has recently come under scrutiny due to the lack of defined dosage parameters and yet unproven short- and long-term benefits and risks to human health. This study quantified the concentration of vinpocetine in several commercially available dietary supplements and found that a highly variable range of 0.6–5.1 mg/serving was present across the tested products, with most products providing no specification of vinpocetine concentrations.

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