Separation and Utilization of Pectin Lyase from Commercial Pectic Enzyme via Highly Methoxylated Cross-Linked Alcohol-Insoluble Solid Chromatography for Wine Methanol Reduction

2007 ◽  
Vol 55 (4) ◽  
pp. 1557-1562 ◽  
Author(s):  
Ming-Chang Wu ◽  
Chii-Ming Jiang ◽  
Ping-Hsiu Huang ◽  
Mei-Yi Wu ◽  
Yuh Tai Wang
Keyword(s):  
Pectin Lyase ◽  
Pectic Enzyme

scholarly journals Cyclic AMP and Low Molecular Weight Effector(s) Present in Yeast Extract Are Involved in Pectin Lyase Production by Penicillium griseoroseum Cultured on Sucrose

1999 ◽  
Vol 76 (2) ◽  
pp. 129-142 ◽  
Author(s):  
Maria Cristina Baracat-Pereira ◽  
Jorge Luiz Cavalcante Coelho ◽  
Rosana Cristina Minussi ◽  
Virgínia Maria Chaves-Alves ◽  
Rogelio Lopes Brandio ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cyclic Amp ◽  
Yeast Extract ◽  
Pectin Lyase ◽  
Low Molecular Weight ◽  
Penicillium Griseoroseum

scholarly journals Effects of pectin lyase-modified red ginseng extracts in high-fat diet-fed obese mice

2014 ◽  
Vol 30 (4) ◽  
pp. 151 ◽  
Author(s):  
Hak-Yong Lee ◽  
Kwang-Hyun Park ◽  
Young-Mi Park ◽  
Dae-In Moon ◽  
Hong-Geun Oh ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Pectin Lyase ◽  
Obese Mice ◽  
Red Ginseng ◽  
High Fat

scholarly journals Partial purification and Characterization of Two Pectinases (Polygalacturonase and Pectin lyase) from Papaya Pericarp (Carica papaya cv. solo 8)

2017 ◽  
Vol 6 (6) ◽  
pp. 2729-2739 ◽  
Author(s):  
Adingra Kouassi Martial Didier ◽  
Konan Kouassi Hubert ◽  
Kouadio Eugène Jean Parfait ◽  
Yapi Jocelyn Constant ◽  
Tano Kablan
Keyword(s):  
Carica Papaya ◽  
Pectin Lyase ◽  
Partial Purification ◽  
Purification And Characterization

scholarly journals Pectin Lyase Production by a Penicillium italicum Strain

1989 ◽  
Vol 55 (6) ◽  
pp. 1612-1616 ◽  
Author(s):  
Aitor Alaña ◽  
Ane Gabilondo ◽  
Fernando Hernando ◽  
Maria D. Moragues ◽  
Juan B. Dominguez ◽  
...  
Keyword(s):  
Pectin Lyase ◽  
Penicillium Italicum

scholarly journals Pectin lyase-modified red ginseng extract exhibits potent anti-glycation effects in vitro and in vivo

2017 ◽  
Vol 21 (2) ◽  
pp. 56-62 ◽  
Author(s):  
Chan-Sik Kim ◽  
Kyuhyung Jo ◽  
Mi-Kyung Pyo ◽  
Jin Sook Kim ◽  
Junghyun Kim
Keyword(s):  
Pectin Lyase ◽  
Red Ginseng ◽  
Ginseng Extract

Fungal pectic enzyme fractionation by dye affinity chromatography

1996 ◽  
Vol 10 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Silvia A. Camperi ◽  
Mariano Grasselli ◽  
Osvaldo Cascone
Keyword(s):  
Affinity Chromatography ◽  
Pectic Enzyme ◽  
Dye Affinity Chromatography

Production and Thermal Characterization of an Alkaline Pectin Lyase from Penicillium notatum

2015 ◽  
Vol 11 (4) ◽  
pp. 517-525 ◽  
Author(s):  
Umme Habibah Siddiqua ◽  
Haq Nawaz Bhatti ◽  
Shazia Nouren ◽  
Saima Noreen ◽  
Ismat Bibi
Keyword(s):  
Ammonium Sulphate ◽  
Thermal Inactivation ◽  
Thermal Characterization ◽  
Maximal Activity ◽  
Inhibitory Effect ◽  
Pectin Lyase ◽  
Partial Purification ◽  
Penicillium Notatum ◽  
Enhanced Production ◽  
Lyase Activity

Abstract The present study was aimed to investigate the potential of Penicillium notatum for the production of pectin lyase under solid state culture using wheat bran as substrate. Different process parameters were optimized using completely randomized design for enhanced production of the pectin lyase. P. notatum showed maximum production (1875 U/gds) of pectin lyase with substrate amount 15 g/250 ml, moisture level 60%, pH 6, incubation period 120 h at 30°C. Pectin lyase activity was further improved with the addition of maltose and ammonium sulphate as carbon and nitrogen additives (1%), respectively. Partial purification of enzyme was carried out by ammonium sulphate precipitation at 80% saturation level. The P. notatum pectin lyase showed maximal activity at 65°C and pH 8. Km and Vmax values were 0.29% and 0.487 µmol/min, respectively. Energy of activation was found to be 5.33 kJ/mol. A detailed kinetic study of thermal inactivation was carried out. The results showed that pectin lyase exhibited resistance against thermal unfolding. Effect of various metals on pectin lyase activity was also investigated. All the metals showed inhibitory effect on the enzyme activity. The present investigation revealed that pectin lyase isolated from P. notatum is thermally stable and alkaline in nature.

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