Broad-Spectrum Antimicrobial Activity in vitro of the Synthetic Peptide D4E1

2001 ◽  
Vol 49 (6) ◽  
pp. 2799-2803 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Kurt D. Stromberg ◽  
Jeffrey W. Cary ◽  
Thomas E. Cleveland
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Synthetic Peptide

scholarly journals Development, Characterization, and Evaluation of Novel Broad-Spectrum Antimicrobial Topical Formulations from Cymbopogon martini (Roxb.) W. Watson Essential Oil

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Negero Gemeda ◽  
Ashenif Tadele ◽  
Hirut Lemma ◽  
Biruktawit Girma ◽  
Getachew Addis ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Broad Spectrum ◽  
Chemical Constituents ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Skin Infections ◽  
Topical Formulations ◽  
Life Study

Background. Skin infections were the most frequently encountered of all infections and the 4th leading cause of nonfatal disease burden. Topical drugs have been used for the management of skin infections. The growing concern of drug resistance to the topical agents has warned the need for continuous development of novel drug. Essential oils are the best candidate for new drug with different mode of action and target as they are rich in chemical constituents. Objective. To evaluate and develop safe and effective topical antimicrobial formulations from essential oil of Cymbopogon martini. Method. Essential oil was extracted using hydrodistillation aerial part C. martini and topical formulations were prepared in five different semisolid bases. In vitro antimicrobial investigations were performed on essential oil and topical formulations. Skin sensitizations of the formulations were evaluated using guinea pig maximization. Results. The essential oil of C. martini has shown broad-spectrum antimicrobial potency against all tested organisms with MIC value ranging from 0.65 to 10 μg/ml. Absolute inhibitions of growth of fungi were observed against Trichophyton mentagrophytes and Trichophyton rubrum at concentrations above 1% of oil and against Microsporum canis and Trichophyton verrucosum at a concentration of 4% oil. Among topical formulations, the highest antimicrobial activity was recorded in hydrophilic ointment followed by macrogol blend ointment. The antimicrobial activity of oil was higher in fungal pathogen compared to bacteria. Gram positive bacteria were more sensitive than gram negative bacteria. Hydrophilic and macrogol blend ointment containing 5% oil did not produce any skin sensitization on guinea pigs. Conclusion. In conclusion, topical formulations of C. martini essential oil can be alternative topical agents with safe broad-spectrum activity for the treatment of skin disorder. Further studies should focus on shelf life study and clinical study of the product.

scholarly journals Potent and Broad-Spectrum Antimicrobial Activity of Analogs from the Scorpion Peptide Stigmurin

2019 ◽  
Vol 20 (3) ◽  
pp. 623 ◽  
Author(s):  
Bruno Amorim-Carmo ◽  
Alessandra Daniele-Silva ◽  
Adriana M. S. Parente ◽  
Allanny A. Furtado ◽  
Eneas Carvalho ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Therapeutic Potential ◽  
Scorpion Venom ◽  
Rational Drug Design ◽  
Biologically Active ◽  
New Drugs ◽  
Structural Conformation ◽  
Native Peptide

Scorpion venom constitutes a rich source of biologically active compounds with high potential for therapeutic and biotechnological applications that can be used as prototypes for the design of new drugs. The aim of this study was to characterize the structural conformation, evaluate the antimicrobial activity, and gain insight into the possible action mechanism underlying it, for two new analog peptides of the scorpion peptide Stigmurin, named StigA25 and StigA31. The amino acid substitutions in the native sequence for lysine residues resulted in peptides with higher positive net charge and hydrophobicity, with an increase in the theoretical helical content. StigA25 and StigA31 showed the capacity to modify their structural conformation according to the environment, and were stable to pH and temperature variation—results similar to the native peptide. Both analog peptides demonstrated broad-spectrum antimicrobial activity in vitro, showing an effect superior to that of the native peptide, being non-hemolytic at the biologically active concentrations. Therefore, this study demonstrates the therapeutic potential of the analog peptides from Stigmurin and the promising approach of rational drug design based on scorpion venom peptide to obtain new anti-infective agents.

Antimicrobial activity of FK-037, a new broad-spectrum cephalosporin international in vitro comparison with cefepime and ceftazidime

1994 ◽  
Vol 18 (3) ◽  
pp. 167-173 ◽  
Author(s):  
Reno Frei ◽  
Ronald N. Jones ◽  
Antonio C. Pignatari ◽  
Nobuhisa Yamane ◽  
Francesc Marco ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
In Vitro Comparison

scholarly journals In Vitro Antimicrobial Activity of MSI-78, a Magainin Analog

1998 ◽  
Vol 42 (5) ◽  
pp. 1213-1216 ◽  
Author(s):  
Peter C. Fuchs ◽  
Arthur L. Barry ◽  
Steven D. Brown
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Bactericidal Activity ◽  
Broad Spectrum ◽  
Vitro Activity ◽  
Topical Agent ◽  
In Vitro Activity ◽  
Cationic Peptide ◽  
In Vitro Antimicrobial Activity

ABSTRACT MSI-78 is a cationic peptide with broad-spectrum antimicrobial activity and is being developed as a topical agent. We compared the in vitro activity of MSI-78 with those of ofloxacin and other antibiotics against fresh clinical isolates. Based on MIC distribution statistics, strains for which the MSI-78 MIC was ≤64 μg/ml were assumed to be susceptible for purposes of this report. Of 411 aerobic isolates tested, 91% were susceptible to MSI-78, compared to 91% for ofloxacin and 92% for ciprofloxacin. Only enterococci consistently required ≥64 μg of MSI-78/ml for inhibition. MSI-78 demonstrated bactericidal activity equivalent to that of ofloxacin. Of 61 anaerobes, 97% were susceptible to MSI-78. Of 10 isolates of Candida albicans, 3 were inhibited by MSI-78 at 24 h. Further studies of this compound appear to be warranted.

scholarly journals Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Henna Sood ◽  
Yashwant Kumar ◽  
Vipan Kumar Gupta ◽  
Daljit Singh Arora
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Broad Spectrum ◽  
In Vitro Cytotoxicity ◽  
Behavioral Pattern ◽  
Root Bark ◽  
Potential Candidate ◽  
Oral Toxicity ◽  
Antibiotic Effect

Abstract Berberis aristata is an important part of traditional healing system from more than 2500 years. The aqueous extract of Berberis aristata root bark displayed broad spectrum activity against 13 test pathogens, ranging from 12 to 25 mm. In classical optimization, 15% concentration prepared at 40 °C for 40 min was optimal and thermostable. Statistical optimization enhanced the activity by 1.13–1.30-folds. Ethyl acetate was the best organic solvent to elute out the potential compound responsible for antimicrobial activity. Diterpenes were the most abundant phytoconstituent (15.3%) and showed broad spectrum antimicrobial activity ranging from 16.66 to 42.66 mm. Ethyl acetate extract displayed the lowest minimum inhibitory concentration (0.05–1 mg/mL), followed by diterpenes (0.05–5 mg/mL) and flavonoids (0.05–10 mg/mL). The test extracts were microbicidal in nature and showed a prolonged post antibiotic effect ranging from 2 to 8 h. They were found to be biosafe as per Ames and MTT assay. The in vitro cytotoxicity evaluation of diterpenes against L20B, RD and Hep 2 cell lines revealed its IC50 ranging from 245 to 473 µg/mL. Acute oral toxicity of diterpenes on Swiss albino mice did not show any changes in behavioral pattern, body weight, biochemical parameters as well as organs’ architecture. The study thus indicates B. aristata could be a potential candidate for development of potent drug owing to its antimicrobial potential and biosafe profile.

scholarly journals EFFICACY EVALUATION OF EXTRACTS OF BRASSICA JUNCEA AND BRASSICA ALBA SEEDS AS POTENTIAL ANTIBACTERIAL AGENT-IN VITRO STUDY, AGAINST PYOGENIC MICROBES - STREPTOCOCCUS PYROGENS, STAPHYLOCOCCUS AUREUS AND E.COLI

2021 ◽  
Vol 9 (7) ◽  
pp. 1344-1348
Author(s):  
Seema Nishant Parma ◽  
Madhusudan N Pawar ◽  
Rehman M. K. ◽  
Madhuri Wagh
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Microbial Activity ◽  
Brassica Juncea ◽  
Broad Spectrum ◽  
In Vitro Study ◽  
Vitro Study ◽  
World Health

The World Health Organization (WHO) reported that about 80% of the world’s population depends primarily on traditional medicine.[1] Ayurveda is the science of life, which aims at building a healthy body with a sound mind. During the past few decades, and in recent Covid-19 Pandaemic situations traditional systems of medicines have become a topic of global importance. The number of emerging multidrug-resistant microbial strains is continuously increasing and has become one of the most serious threats to the successful treatment of infectious diseases. In this study, the antibacterial potentials of the extracts of water, Petroleum ether, methanol & Chloroform of Brassica juncea & Brassica alba seeds were tested against mentioned pyogenic bacteria. Given reports of Brassica species in the treatment of various infections since the Vedic period & Ayurveda, the seed oil had been investigated for its anti-microbial activity by several research workers. Some herbs from Rakshoghna Gana [2] were screened for their in vitro anti-microbial activity on E.Coli and Staphylococcus Aureus. Hence as a part of further research, Brassicajuncea and Brassica alba various extracts and crude oils were evaluated for antibacterial activity against pyogenic bacteria Streptococcus aureus, Staphylococcus aureus and E.Coli by Agar Cup Dilution Technique against standard chemotherapeutic agents, by using 12 antibiotics, sensitivity discs. In vitro study revealed out that crude oil of Brassica juncea and Brassica alba is not potent as standard antimicrobial agents, but water extract and methanol extract possess significant antimicrobial activity in Vitro. Both the extract possesses antimicrobial activity against all 3 pyogenic bacteria and potent as standard 12 antimicrobial drugs. The extracts showed a broad spectrum of antibacterial activity, inhibition zones of bacteria strains ranged from 3-12 mm for Staphylococcus aureus,3-15mm for Streptococcus pyrogens 5-12mm for E. coli. The result of the study supports the immense medicinal properties of Brassica juncea which has revealed a significant scope to develop a novel broad spectrum of antibacterial herbal formulation. Again Brassica alba was found superior in antibacterial action as compared to Brassica juncea as its mention in Ayurved Lexicon Bhavprakash Nighantu,[3,4] results substantiate the same. This is further open for re- search workers to carry out further research work on this project and to see the phagocytic action of the drug, fix the dose of the drug, fractionization of water and methanol extracts by HPTLC separation of active principle as further drug development. Keywords: Brassica juncea, Brassica alba, antibacterials agents, herbal medicine.

scholarly journals In vitro antibacterial activity of essential oils from Lamiaceae species

2016 ◽  
Vol 5 (4) ◽  
pp. 141-144
Author(s):  
Vinod Kumar ◽  
◽  
C. S. Mathela ◽  
Amit Panwar ◽  
◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Broad Spectrum ◽  
Antibacterial Effect ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Negative ◽  
In Vitro Antibacterial Activity

Essential oils from Calamintha umbrosa and Nepeta species viz. N. leucophylla; N. hindostana; N. ciliaris and N. clarkei (family Lamiaceae), was tested against six bacterial strains. To evaluate the correlation between the antimicrobial activity and the essential oils, PCA and HCA analysis was done. PCA and HCA analysis of the antibacterial activity revealed that essential oils of Nepeta species had a strong and broad spectrum antibacterial effect against bacterial strains of P. aeruginosa and S. scandidus. The N. leucophylla oil showed higher activity against Gram-negative bacteria P. aeruginosa (10.5 mm, MIC 10 µL/mL) and K. pneumonia (9.1 mm, MIC 45 µL/mL) among all Nepeta oils which may be due to presence of active antimicrobial iridoids compounds.

scholarly journals Facile Synthesis, Characterization, and In Vitro Antimicrobial Screening of a New Series of 2,4,6-Trisubstituted-s-triazine Based Compounds

2015 ◽  
Vol 2015 ◽  
pp. 1-10
Author(s):  
Ravi Bhushan Singh ◽  
Nirupam Das ◽  
Md. Kamaruz Zaman
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Elemental Analysis ◽  
Broad Spectrum ◽  
Antibacterial Efficacy ◽  
Facile Synthesis ◽  
Standard Drug ◽  
E Coli

A series of new 2,4,6-trisubstituted-s-triazine was synthesized, assessed for antimicrobial activity, and characterized by FTIR, 1HNMR, 13CNMR, and elemental analysis. The tested compounds, 4d, 4g, 4h, 4k, and 4n, have shown considerable in vitro antibacterial efficacy with reference to the standard drug ciprofloxacin (MIC 3.125 μgmL−1 against B. subtilis, E. coli, and K. pneumoniae). It was observed that compounds 4d and 4h displayed equipotent antibacterial efficacy against B. subtilis (MIC 3.125 μgmL−1) and S. aureus (MIC 6.25 μgmL−1). The studies demonstrated that the para-fluorophenylpiperazine substituted s-triazine (4n) was potent and exhibited broad spectrum antibacterial activity against S. epidermidis, K. pneumoniae, and P. aeruginosa with MIC of 6.25 μgmL−1 and for E. coli, it showed an MIC of 3.125 μgmL−1 equipotent with reference to the standard drug. Among all the compounds under investigation, compound 4g also demonstrated significant antifungal activity (3.125 μgmL−1) against C. albicans.

scholarly journals Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 866
Author(s):  
Carlos C. F. Pleeging ◽  
Tom Coenye ◽  
Dimitris Mossialos ◽  
Hilde de Rooster ◽  
Daniela Chrysostomou ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Wound Healing ◽  
Broad Spectrum ◽  
Biofilm Formation ◽  
Wound Care ◽  
Multidrug Resistant ◽  
Synergistic Activity ◽  
Medical Grade

Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements.

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