Stabilization of carotene and xanthophyll in alfalfa leaf protein concentrates

1982 ◽  
Vol 30 (5) ◽  
pp. 934-937 ◽  
Author(s):  
Cameron K. Lyon ◽  
George O. Kohler
Keyword(s):  
Leaf Protein ◽  
Protein Concentrates ◽  
Alfalfa Leaf

Quantitative Determination of Coumestrol in Dried Alfalfa and Alfalfa Leaf Protein Concentrates Containing Chlorophyll

1975 ◽  
Vol 58 (5) ◽  
pp. 983-986
Author(s):  
Benny E Knuckles ◽  
Raymond E Miller ◽  
E M Bickoff
Keyword(s):  
Quantitative Determination ◽  
Analytical Method ◽  
Leaf Protein ◽  
Improved Method ◽  
Alcohol Extract ◽  
Protein Concentrates ◽  
Coefficients Of Variation ◽  
Lower Standard ◽  
Alfalfa Leaf

Abstract An improved analytical method for the determination of coumestrol in dried alfalfa and leaf protein concentrates is described. In this method, chlorophyll is removed from an alcohol extract prior to the paper chromatographic-fluorometric measurement of coumestrol. Ninety-eight per cent of the coumestrol added to alfalfa leaf protein concentrates is recovered by this method. This improved method gives replicate values with lower standard deviations and coefficients of variation than the literature method.

The use of alfalfa leaf protein concentrates as a protein source in diets for tilapia (Oreochromis mossambicus)

Aquaculture ◽  
1990 ◽  
Vol 90 (3-4) ◽  
pp. 291-302 ◽  
Author(s):  
Miguel A. Olvera-Novoa ◽  
Silvia G. Campos ◽  
Mirna G. Sabido ◽  
Carlos A. Martínez Palacios
Keyword(s):  
Oreochromis Mossambicus ◽  
Protein Source ◽  
Leaf Protein ◽  
Protein Concentrates ◽  
Alfalfa Leaf

Available carbohydrates in alfalfa leaf protein concentrates

1994 ◽  
Vol 42 (8) ◽  
pp. 1747-1749 ◽  
Author(s):  
Teresa Hernandez ◽  
Amelia Hernandez
Keyword(s):  
Leaf Protein ◽  
Protein Concentrates ◽  
Alfalfa Leaf

Microbiological Assay for Saponin in Alfalfa Products

1977 ◽  
Vol 60 (4) ◽  
pp. 957-960 ◽  
Author(s):  
A Lyle Livingston ◽  
Linda C Whitehand ◽  
George O Kohler
Keyword(s):  
Aqueous Solution ◽  
Trichoderma Viride ◽  
Potato Dextrose Agar ◽  
Leaf Protein ◽  
Ratio Analysis ◽  
Slope Ratio ◽  
Protein Concentrates ◽  
Alfalfa Leaf ◽  
Alfalfa Sprouts ◽  
Ethanol Ethanol

Abstract A bioassay is described for determining medicagenin-type saponin in dried alfalfa, leaf protein concentrates, and alfalfa sprouts. Samples are extracted by refluxing 2½ hr with 50% ethanol, ethanol is evaporated, and aliquots of an aqueous solution are added to potato dextrose agar (PDA) and assayed for saponin by using the fungus Trichoderma viride. The growth of the fungus on PDA is compared with a standard saponin, and saponin levels are calculated by means of a slope ratio analysis.

THE POLY-PROTEIN PROCESS: A NEW METHOD FOR OBTAINING LEAF PROTEIN CONCENTRATES

1977 ◽  
Vol 42 (5) ◽  
pp. 1401-1401 ◽  
Author(s):  
G. ANELLI ◽  
R. FIORENTINI ◽  
L. MASSIGNAN ◽  
C. GALOPPINI
Keyword(s):  
New Method ◽  
Leaf Protein ◽  
Protein Concentrates

scholarly journals High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry

2021 ◽  
Author(s):  
Ajay Iyer ◽  
Lisa Guerrier ◽  
Salomé Leveque ◽  
Charles S. Bestwick ◽  
Sylvia H. Duncan ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Extraction ◽  
Leaf Protein ◽  
Protein Recovery ◽  
Screening Methods ◽  
Extraction Technology ◽  
Protein Concentrates ◽  
Ethanol Precipitation

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.

Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate

2008 ◽  
Vol 111 (2) ◽  
pp. 370-376 ◽  
Author(s):  
Zhengjun Xie ◽  
Junrong Huang ◽  
Xueming Xu ◽  
Zhengyu Jin
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Leaf Protein ◽  
Alfalfa Leaf
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