A new method for determination of insoluble cell walls and soluble nonstarchy polysaccharides from plant materials

1988 ◽  
Vol 36 (5) ◽  
pp. 969-979 ◽  
Author(s):  
Jean Marc Brillouet ◽  
Xavier Rouau ◽  
Christine Hoebler ◽  
Jean Luc Barry ◽  
Bernard Carre ◽  
...  
Keyword(s):  
Cell Walls ◽  
New Method ◽  
Plant Materials

A new method for the determination of lolitrem B in plant materials

2014 ◽  
Vol 193 ◽  
pp. 141-147 ◽  
Author(s):  
Céline Repussard ◽  
Didier Tardieu ◽  
Mélanie Alberich ◽  
Philippe Guerre
Keyword(s):  
New Method ◽  
Plant Materials ◽  
Lolitrem B

scholarly journals Determination of the complex of cell wall substances in plant products

1965 ◽  
Vol 37 (4) ◽  
pp. 305-312
Author(s):  
L. Paloheimo ◽  
K. A. Vainio
Keyword(s):  
Cell Wall ◽  
Acid Treatment ◽  
Dry Matter ◽  
Cold Water ◽  
Weak Acid ◽  
New Method ◽  
Absolute Ethanol ◽  
Neutral Sugar ◽  
Plant Materials

The authors present a new method for the determination of the complex of vegetable cell wall substances. The sample is extracted with boiling 80 % ethanol, boiling absolute ethanol and cold water. The residue corrected for ash, protein, and, if necessary, for starch, gives the amount of cell wall substances. Determinations were made of the same samples of which Salo in this department, using quite a different principle, has determined the cell wall complex. She determined separately cellulose, neutral sugar hemicellulose, uronic acid hemicellulose, and lignin. Adding up these items Salo obtained the total of the cell wall substances. The results obtained with the new method are in most cases in agreement with the results of Salo (Table 1). The 80 % ethanol seems to be a very efficient solvent. In most cases more than 35 % of the dry matter of the sample was dissolved by it, while only about 0.3 % was dissolved in the succeeding extraction with absolute ethanol (Table 2). 1—12 % was dissolved by water. The new method is compared also with the earlier method of Paloheimo in which the sample is boiled in 0.05 N hydrochloric acid. It appeared that the results obtained with the latter procedure are considerably lower than those obtained with the new method. Evidently most plant materials contain cell wall substances which are extractable with a very weak acid treatment.

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method

A New Method for the Determination of Plasma Activators of Fibrinolysis

1977 ◽  
Vol 37 (02) ◽  
pp. 210-215 ◽  
Author(s):  
R Margalit ◽  
E Gidron ◽  
Y Shalitin
Keyword(s):  
New Method ◽  
Effective Activator

SummaryThe term “effective activator” of plasminogen is proposed, to denote the resultant of activator-antiactivator interaction, and a method for the determination of the level of these activators is described. By adding axcess plasminogen to the euglobulin fraction of plasma the influence of the level of endogenous plasminogen and of the antiplasmin is eliminated. It is shown that the level of fibrinogen has very little bearing on the results. An effective activator unit is defined as equal to 1 CTA unit of urokinase activity on a fibrinogen-plasminogen substrate.

The Plasmin Inhibitors of Plasma

1964 ◽  
Vol 12 (01) ◽  
pp. 119-125 ◽  
Author(s):  
Y Shamash ◽  
A Rimon
Keyword(s):  
Human Plasma ◽  
New Method ◽  
Normal Amount ◽  
Caseinolytic Activity ◽  
Before And After ◽  
Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

Development of a New Method for Determination of the Oil Content from Microalgae Lipid Fraction

2017 ◽  
Vol 68 (4) ◽  
pp. 671-674
Author(s):  
Ana Maria Galan ◽  
Ioan Calinescu ◽  
Elena Radu ◽  
Elena Emilia Oprescu ◽  
Gabriel Vasilievici ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Oil Content ◽  
Lipid Fraction ◽  
New Method ◽  
Ms Analysis ◽  
Qualitative Determination ◽  
Tga Analysis

The purpose of this study was to develop a method for rapid quantitative and qualitative determination of the oil from microalgae lipid fraction obtained from Nannochloris sp biomass. The lipid fraction was first refluxed with 4% KOH in MeOH (60, 90, 120 min), followed by reaction with 20% BF3 in MeOH, using different times of reflux (90,120, 150 min) for each time of reflux with 4% KOH in MeOH. The FAME samples were analyzed by GC-MS analysis. 120 min reflux with 4% KOH in MeOH, 90 min with 20% BF3 in MeOH and a ratio lipid fraction: 4% KOH in MeOH: 20% BF3 in MeOH=1:20:27, were required to obtain the higher percent of oil in the microalgae lipid fraction. The relevance of the method developed was proved by TGA analysis and by transesterification of a sunflower oil sample in the same conditions.

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