Application of in vitro methods to assess the nutritive value of leaf protein concentrates

1987 ◽  
Vol 35 (1) ◽  
pp. 82-85 ◽  
Author(s):  
Chitra Savangikar ◽  
Mitsuaki Ohshima
Keyword(s):  
Nutritive Value ◽  
Leaf Protein ◽  
In Vitro Methods ◽  
Protein Concentrates

scholarly journals In vivo and in vitro methods of measuring nutritive value of leaf-protein preparations

1969 ◽  
Vol 23 (3) ◽  
pp. 533-545 ◽  
Author(s):  
R. A. Buchanan
Keyword(s):  
Nutritive Value ◽  
Unsaturated Fatty Acids ◽  
Lipid Extraction ◽  
Leaf Protein ◽  
In Vitro Methods ◽  
Freeze Dried ◽  
True Digestibility ◽  
Net Protein

1. Several in vitro methods were compared with in vivo methods for estimating the nutritive value of leaf-protein concentrates (LPC), using a freeze-dried preparation from wheat (A) fresh, (B) after heating moist, (C) after heating moist and then extraction with chloroform, and (D) after extraction with an acidified solvent.2. The treatments had little effect on the biological value (BV) of the samples for rats.3. Heating moist decreased true digestibility (TD), net protein utilization (NPU) and protein efficiency ratio (PER), but the original values were almost restored by lipid extraction. Acidified solvent extraction decreased TD, NPU and PER of LPC perhaps by making it brittle and difficult to wet.4. Papain solubility and TD were well correlated. Pepsin-pancreatin solubility and TD were less well correlated.5. Microbiological estimations of available amino acids, involving predigestion with pepsin, correlated poorly with TD determinations.6. Unsaturated fatty acids, particularly linolenic, formed complexes during heating of LPC. The effect of this on enzyme solubilization procedures and on digestion in vivo is discussed.7. Some comparisons are made between the effect of heat and of extraction with solvents on LPC and on fish meal.

Measurement of Digestibility of Alfalfa Protein Concentrates by in vivo and in vitro Methods

1973 ◽  
Vol 103 (4) ◽  
pp. 530-535 ◽  
Author(s):  
R. M. Saunders ◽  
M. A. Connor ◽  
A. N. Booth ◽  
E. M. Bickoff ◽  
G. O. Kohler
Keyword(s):  
In Vitro Methods ◽  
Protein Concentrates

Yield, composition and nutritive value of leaf protein concentrates from mixed forages of cereals and legumes

1989 ◽  
Vol 27 (1-2) ◽  
pp. 117-125 ◽  
Author(s):  
ElŻbieta Pisulewska ◽  
Piotr Hanczakowski ◽  
PaweŁ Pisulewski
Keyword(s):  
Nutritive Value ◽  
Leaf Protein ◽  
Protein Concentrates

A Study of the Chemical Composition and Nutritive Value of Leaf Protein Concentrates

1972 ◽  
Vol 5 (2) ◽  
pp. 50-54 ◽  
Author(s):  
M. Tao ◽  
M. Boulet ◽  
G.J. Brisson ◽  
K.H. Huang ◽  
R.R. Riel ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Leaf Protein ◽  
Protein Concentrates

In vitrotechniques as tools to predict nutrient supply in ruminants

1998 ◽  
Vol 22 ◽  
pp. 1-11 ◽  
Author(s):  
S. Tamminga ◽  
B. A. Williams
Keyword(s):  
Nutritive Value ◽  
Farm Animals ◽  
In Vitro Techniques ◽  
Evaluation Systems ◽  
In Vitro Methods ◽  
Future Developments ◽  
In Vitro Systems ◽  
High Degree

AbstractIn vitro techniques are widely used to predict the nutritive value of foods for farm animals. However, food evaluation systems are moving towards systems based on nutrient flows rather than on energy or protein. Nutrients are supplied via the digestive tract and presently available in vitro methods are inadequate to simulate the complicated and non-steady-state processes in this tract. This is partly due the lack of adequate in vivo reference values and partly due to a too-high degree of standardization and simplification. Future developments should therefore aim to develop in vitro systems which closely monitor the dynamics of the digestive processes. Adequate interpretation of the results may require sophisticated mathematical models.

Nutritive value of leaf protein concentrates. II.

1956 ◽  
Vol 7 (12) ◽  
pp. 775-780 ◽  
Author(s):  
S. J. Cowlishaw ◽  
D. E. Eyles ◽  
W. F. Raymond ◽  
J. M. A. Tilley
Keyword(s):  
Nutritive Value ◽  
Leaf Protein ◽  
Protein Concentrates

NUTRITIVE VALUE OF LEAF PROTEIN CONCENTRATES PREPARED FROM Amaranthus SPECIES

1981 ◽  
Vol 61 (1) ◽  
pp. 199-204 ◽  
Author(s):  
P. R. CHEEKE ◽  
R. CARLSSON ◽  
G. O. KOHLER
Keyword(s):  
Soybean Meal ◽  
Nutritive Value ◽  
Phenolic Content ◽  
Control Diet ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Leaf Protein ◽  
Poor Growth ◽  
The Poor

Leaf protein concentrate (LPC) samples were prepared from several species of Amaranthus. Their proximate composition and phenolic content were determined. In vitro nitrogen digestibility was low (28–48%) and was negatively correlated with phenolic content. The amino acid content of one sample of A. hypochondriacus LPC was measured, and compared favorably with reported values for essential amino acids for alfalfa LPC and soybean meal. The nutritional values of the LPC samples were assessed in a rat feeding trial. Compared to a soybean meal control diet, the gains with Amaranthus LPC were poor, ranging from 18 to 72% of the control value. The cause of the poor growth remains to be determined. The high ash content of the samples, or the presence of organic factors such as saponins, phenolics and oxalates are possibilities to account for the poor growth rate of rats.

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