Phase-Equilibria in Methanol-Ethyl Acetate-Water System.

1963 ◽  
Vol 8 (4) ◽  
pp. 484-490 ◽  
Author(s):  
Kiyomi Akita ◽  
Fumitake Yoshida
1949 ◽  
Vol 41 (10) ◽  
pp. 2352-2358 ◽  
Author(s):  
John Griswold ◽  
Pao L. Chu ◽  
W. O. Winsauer

2001 ◽  
Vol 46 (2) ◽  
pp. 381-384 ◽  
Author(s):  
Yu-Taek Seo ◽  
Huen Lee ◽  
Ji-Ho Yoon

Langmuir ◽  
2013 ◽  
Vol 29 (47) ◽  
pp. 14369-14379 ◽  
Author(s):  
G. Montalvo ◽  
R. Pons ◽  
G. Zhang ◽  
M. Díaz ◽  
M. Valiente

1952 ◽  
Vol 44 (3) ◽  
pp. 609-615 ◽  
Author(s):  
H. H. Reamer ◽  
B. H. Sage ◽  
W. N. Lacey

2020 ◽  
Vol 65 (9) ◽  
pp. 4684-4691 ◽  
Author(s):  
Mohammad Pourranjbar ◽  
Hassan Pahlavanzadeh ◽  
Ali Askari Zadeh Mahani ◽  
Amir H. Mohammadi

1957 ◽  
Vol 35 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Allan G. Gornall ◽  
Cynthia Gwilliam

Details are given of a method which has proved very satisfactory for the isolation of aldosterone from commercial aqueous adrenal cortical extracts. The hormone was extracted with chloroform and separated by chromatography in the following systems: System I, benzene: 5% ethyl acetate/methanol: water; System II, toluene/propylene glycol; System III, benzene: 10% ethyl acetate/methanol: water. Identification of the isolated steroid was provided by: U.V. absorption, acetylation followed by chromatography, bio-assay, qualitative tests with blue tetrazolium and phenylhydrazine, absorption spectra in sulphuric and phosphoric acids, and crystallization. Quantitative estimations were made by U.V. absorption and the dinitrophenylhydrazine reaction. The latter method has been shown to exhibit with aldosterone a characteristic behavior that can assist in establishing the identity of this hormone. The yield from the extract principally employed was 4 μg. per ml.


Sign in / Sign up

Export Citation Format

Share Document