Sample Preparation Free Mass Spectrometry Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry: Applications to Microbiology, Metabolic Biofluid Phenotyping, and Food Authenticity

2021 ◽  
Author(s):  
Simon J. S. Cameron ◽  
Alvaro Perdones-Montero ◽  
Lieven Van Meulebroek ◽  
Adam Burke ◽  
Kate Alexander-Hardiman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Food Authenticity

Comprehensive analysis of Ginkgo tablets by easy ambient sonic spray ionization mass spectrometry

2013 ◽  
Vol 91 (8) ◽  
pp. 671-678 ◽  
Author(s):  
Endler Marcel Borges ◽  
Dietrich A. Volmer ◽  
Marcos N. Eberlin
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Comprehensive Analysis ◽  
Mass Spectrometric ◽  
Flavonol Glycosides ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Active Components ◽  
Sonic Spray Ionization ◽  
Mass Spectrometric Techniques

We report here that easy ambient sonic spray ionization − mass spectrometry (EASI−MS) allowed direct, rapid, and comprehensive analysis of active components (e.g., terpenetrilactones and intact flavonol glycosides) in Ginkgo tablets. Unlike conventional methods that hydrolyze flavonol glycosides to flavonoids prior to analysis, EASI−MS detects intact flavonol glycosides directly from the tablets enabling differentiation of these natural glycosides from the synthetic flavonoids. Adulteration of Ginkgo tablets is therefore readily recognized in less than 10 s measurement time. Sample preparation is simple and requires only 0.5 mL of methanol and a mortar and pestle. Additionally, no reference standards are needed and a comparison is made to a certified Ginkgo tablet. To demonstrate these abilities, 22 commercial Ginkgo tablets were analyzed by EASI−MS. The data revealed large variations of ingredients and strong deviations from the specified Ginkgo extract levels. These results emphasize the importance of monitoring Ginkgo products using appropriate methodologies as suggested in our work. The technique may replace more demanding LC−MS/MS methods, which can achieve comparable results but are considerably more labor-intensive. Importantly, the presented method is cheaper, faster, and much simpler to operate than chromatography-based mass spectrometric techniques.

scholarly journals Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS)

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3495-3502 ◽  
Author(s):  
J. K. Park ◽  
C. D. M Campos ◽  
P. Neužil ◽  
L. Abelmann ◽  
R. M. Guijt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Electrospray Ionization ◽  
Mass Spectrometer ◽  
Electrospray Ionization Mass Spectrometry ◽  
Direct Coupling ◽  
Free Flow ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Esi Ms

The direct coupling of free-flow isotachophoresis device with an electrospray-ionization-mass-spectrometer allows continuous online detection without any sample preparation such as purification and labeling.

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