scholarly journals Iridium Nanoparticles for Multichannel Luminescence Lifetime Imaging, Mapping Localization in Live Cancer Cells

2018 ◽  
Vol 140 (32) ◽  
pp. 10242-10249 ◽  
Author(s):  
Siobhan M. King ◽  
Sunil Claire ◽  
Rodolfo I. Teixeira ◽  
Abiola N. Dosumu ◽  
Andrew J. Carrod ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Luminescence Lifetime ◽  
Iridium Nanoparticles ◽  
Lifetime Imaging

scholarly journals Fluorescence Lifetime Imaging Microcopy of Extravasating Cancer Cells in the Mouse Microenvironment

2012 ◽  
Vol 102 (3) ◽  
pp. 191a
Author(s):  
Michelle A. Digman ◽  
Atsushi Suetsugu ◽  
Federica Sabatini ◽  
Robert M. Hoffman ◽  
Enrico Gratton
Keyword(s):  
Cancer Cells ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Cannabidiol Modulates Mitochondrial Redox and Dynamics in MCF7 Cancer Cells: A Study Using Fluorescence Lifetime Imaging Microscopy of NAD(P)H

2021 ◽  
Vol 8 ◽  
Author(s):  
Rhys Richard Mould ◽  
Stanley W. Botchway ◽  
James R. C. Parkinson ◽  
Elizabeth Louise Thomas ◽  
Geoffrey W Guy ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescence Lifetime ◽  
Mitochondrial Function ◽  
Mode Of Action ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Mitochondrial Morphology ◽  
Fluorescence Lifetime Imaging ◽  
Dependent Manner ◽  
Lifetime Imaging ◽  
Dose Dependent

The cannabinoid, cannabidiol (CBD), is part of the plant's natural defense system that when given to animals has many useful medicinal properties, including activity against cancer cells, modulation of the immune system, and efficacy in epilepsy. Although there is no consensus on its precise mode of action as it affects many cellular targets, CBD does appear to influence mitochondrial function. This would suggest that there is a cross-kingdom ability to modulate stress resistance systems that enhance homeostasis. As NAD(P)H autofluorescence can be used as both a metabolic sensor and mitochondrial imaging modality, we assessed the potential of this technique to study the in vitro effects of CBD using 2-photon excitation and fluorescence lifetime imaging microscopy (2P-FLIM) of NAD(P)H against more traditional markers of mitochondrial morphology and cellular stress in MCF7 breast cancer cells. 2P-FLIM analysis revealed that the addition of CBD induced a dose-dependent decrease in bound NAD(P)H, with 20 µM treatments significantly decreased the contribution of bound NAD(P)H by 14.6% relative to the control (p < 0.001). CBD also increased mitochondrial concentrations of reactive oxygen species (ROS) (160 ± 53 vs. 97.6 ± 4.8%, 20 µM CBD vs. control, respectively, p < 0.001) and Ca2+ (187 ± 78 vs. 105 ± 10%, 20 µM CBD vs. the control, respectively, p < 0.001); this was associated with a significantly decreased mitochondrial branch length and increased fission. These are all suggestive of mitochondrial stress. Our results support the use of NAD(P)H autofluorescence as an investigative tool and provide further evidence that CBD can modulate mitochondrial function and morphology in a dose-dependent manner, with clear evidence of it inducing oxidative stress at higher concentrations. This continues to support emerging data in the literature and may provide further insight into its overall mode of action, not only in cancer, but potentially its function in the plant and why it can act as a medicine.

scholarly journals Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

10.3791/60191 ◽  
2019 ◽  
Author(s):  
Maria Moßhammer ◽  
Vincent V. Scholz ◽  
Gerhard Holst ◽  
Michael Kühl ◽  
Klaus Koren
Keyword(s):  
Frequency Domain ◽  
Luminescence Lifetime ◽  
Camera System ◽  
Lifetime Imaging

A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

1998 ◽  
Vol 51 (1-3) ◽  
pp. 163-170 ◽  
Author(s):  
Gerhard Holst ◽  
Oliver Kohls ◽  
Ingo Klimant ◽  
Bettina König ◽  
Michael Kühl ◽  
...  
Keyword(s):  
Biological Samples ◽  
Imaging System ◽  
Luminescence Lifetime ◽  
Oxygen Distribution ◽  
Lifetime Imaging

Luminescence Lifetime Imaging of Oxygen, pH, and Carbon Dioxide Distribution Using Optical Sensors

2000 ◽  
Vol 54 (4) ◽  
pp. 548-559 ◽  
Author(s):  
Gregor Liebsch ◽  
Ingo Klimant ◽  
Bernhard Frank ◽  
Gerhard Holst ◽  
Otto S. Wolfbeis
Keyword(s):  
Optical Sensors ◽  
Optical Sensor ◽  
Luminescence Lifetime ◽  
Modular System ◽  
Background Suppression ◽  
Time Resolved ◽  
Typical Application ◽  
Lifetime Imaging ◽  
Led Array ◽  
Decay Times

We present a modular system for time-resolved two-dimensional luminescence lifetime imaging of planar optical chemical sensors. It is based on a fast, gateable charge-coupled device (CCD) camera without image intensifier and a pulsable light-emitting diode (LED) array as a light source. Software was developed for data acquisition with a maximum of parameter variability and for background suppression. This approach allows the operation of the system even under daylight. Optical sensors showing analyte-specific changes of their luminescence decay time were tested and used for sensing pO2, pCO2, pH, and temperature. The luminophores employed are either platinum(II)-porphyrins or ruthenium(II)-polypyridyl complexes, contained in polymer films, and can be efficiently excited by blue LEDs. The decay times of the sensor films vary from 70 μs for the Pt(II)-porphyrins to several 100 ns for the Ru(II) complexes. In a typical application, 7 mm-diameter spots of the respective optical sensor films were placed at the bottom of the wells of microtiterplates. Thus, every well represents a separate calibration chamber with an integrated sensor element. Both luminescence intensity-based and time-resolved images of the sensor spots were evaluated and compared. The combination of optical sensor technology with time-resolved imaging allows a determination of the distribution of chemical or physical parameters in heterogeneous systems and is therefore a powerful tool for screening and mapping applications.

A bimodal fluorescent and photocytotoxic naphthalene diimide for theranostic applications

2016 ◽  
Vol 14 (30) ◽  
pp. 7238-7249 ◽  
Author(s):  
Erica Salvati ◽  
Filippo Doria ◽  
Francesco Manoli ◽  
Carmen D'Angelo ◽  
Annamaria Biroccio ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Cancer Cells ◽  
Fluorescence Lifetime ◽  
Red Light ◽  
Water Soluble ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Light Emitter ◽  
Naphthalene Diimide ◽  
Theranostic Applications

We describe the bimodal activity of a water-soluble tetracationic naphthalene diimide as red light emitter for fluorescence imaging, including fluorescence-lifetime imaging, and singlet oxygen photosensitizer, inducing photocytotoxicity in cancer cells.

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