scholarly journals Catalytically Active Single-Chain Polymeric Nanoparticles: Exploring Their Functions in Complex Biological Media

2018 ◽  
Vol 140 (9) ◽  
pp. 3423-3433 ◽  
Author(s):  
Yiliu Liu ◽  
Sílvia Pujals ◽  
Patrick J. M. Stals ◽  
Thomas Paulöhrl ◽  
Stanislav I. Presolski ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Single Chain ◽  
Biological Media ◽  
Catalytically Active

scholarly journals Topology and quaternary structure of pro-sucrase/isomaltase and final-form sucrase/isomaltase

1986 ◽  
Vol 237 (2) ◽  
pp. 455-461 ◽  
Author(s):  
G M Cowell ◽  
J Tranum-Jensen ◽  
H Sjöström ◽  
O Norén
Keyword(s):  
Electron Microscopy ◽  
Quaternary Structure ◽  
Functional Organization ◽  
Enzyme Complex ◽  
Single Chain ◽  
Pancreatic Duct Ligation ◽  
Duct Ligation ◽  
Catalytically Active ◽  
Identical Series ◽  
Dimeric Model

Pig sucrase/isomaltase (EC 3.2.1.48/10) was purified from intestinal microvillar vesicles prepared from animals with and without pancreatic-duct ligation to obtain the single-chain pro form and the proteolytically cleaved final form respectively. The purified enzymes were re-incorporated into phosphatidylcholine vesicles and analysed by electron microscopy after negative staining. The two forms of the enzyme were observed as identical series of characteristic projected views that could be unified in a single dimeric model, containing two sucrase and two isomaltase units. This shows a homodimeric functional organization similar to that of other microvillar hydrolases. The bulk of the dimer was separated from the membrane by a maximal gap of 3.5 nm, representing a junctional segment connecting the intramembrane section of the anchor to the catalytically active domain of sucrase/isomaltase. The enzyme complex protrudes from the membrane for a distance of up to 17 nm. From charge-shift immunoelectrophoresic studies of hydrophilic prosucrase/isomaltase and from electron microscopy of reconstituted pro-sucrase/isomaltase, there was no evidence to suggest the presence of anchoring sequences between the sucrase and isomaltase subunits.

scholarly journals Catalytically Active N -Heterocyclic Carbene Release from Single-Chain Nanoparticles Following a Thermolysis-Driven Unfolding Strategy

2019 ◽  
Vol 40 (15) ◽  
pp. 1970033
Author(s):  
Sofiem Garmendia ◽  
Stefan B. Lawrenson ◽  
Maria C. Arno ◽  
Rachel K. O'Reilly ◽  
Daniel Taton ◽  
...  
Keyword(s):  
Single Chain ◽  
Catalytically Active

scholarly journals Surface water retardation around single-chain polymeric nanoparticles: critical for catalytic function?

2016 ◽  
Vol 7 (3) ◽  
pp. 2011-2015 ◽  
Author(s):  
Patrick J. M. Stals ◽  
Chi-Yuan Cheng ◽  
Lotte van Beek ◽  
Annelies C. Wauters ◽  
Anja R. A. Palmans ◽  
...  
Keyword(s):  
Surface Water ◽  
Functional Groups ◽  
Polymeric Nanoparticles ◽  
Water Soluble ◽  
Single Chain ◽  
Catalytic Function ◽  
Radical Polymerisation

A library of water-soluble dynamic single-chain polymeric nanoparticles (SCPN) was prepared using a controlled radical polymerisation technique followed by the introduction of functional groups, including probes at targeted positions.

Depolymerizable, adaptive supramolecular polymer nanoparticles and networks

2014 ◽  
Vol 5 (12) ◽  
pp. 3788-3794 ◽  
Author(s):  
Joshua A. Kaitz ◽  
Catherine M. Possanza ◽  
Yang Song ◽  
Charles E. Diesendruck ◽  
A. Jolanda H. Spiering ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Polymer Networks ◽  
Polymer Nanoparticles ◽  
Cross Linking ◽  
Supramolecular Polymer ◽  
Single Chain ◽  
Degradable Polymer

Depolymerizable polymers are appended with supramolecular cross-linking motifs to enable preparation of tunable single-chain polymeric nanoparticles and degradable polymer networks.

Water-Soluble Single-Chain Polymeric Nanoparticles for Highly Selective Cancer Chemotherapy

2020 ◽  
Author(s):  
Chih-Chia Cheng ◽  
Shan-You Huang ◽  
Wen-Lu Fan ◽  
Ai-Wei Lee ◽  
Chih-Wei Chiu ◽  
...  
Keyword(s):  
Cancer Chemotherapy ◽  
Polymeric Nanoparticles ◽  
Water Soluble ◽  
Single Chain
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