scholarly journals Drivers of i-DNA Formation in a Variety of Environments Revealed by Four-Dimensional UV Melting and Annealing

2021 ◽  
Author(s):  
Mingpan Cheng ◽  
Jielin Chen ◽  
Huangxian Ju ◽  
Jun Zhou ◽  
Jean-Louis Mergny
Keyword(s):  
Uv Melting

UV Melting of G‐Quadruplexes

2009 ◽  
Vol 37 (1) ◽  
Author(s):  
Jean‐Louis Mergny ◽  
Laurent Lacroix
Keyword(s):  
Uv Melting

pH and Temperature-Induced Molten Globule-Like Denatured States of Equinatoxin II:  A Study by UV-Melting, DSC, Far- and Near-UV CD Spectroscopy, and ANS Fluorescence†

Biochemistry ◽  
1997 ◽  
Vol 36 (47) ◽  
pp. 14345-14352 ◽  
Author(s):  
Nataša Poklar ◽  
Jurij Lah ◽  
Mateja Salobir ◽  
Peter Maček ◽  
Gorazd Vesnaver
Keyword(s):  
Molten Globule ◽  
Cd Spectroscopy ◽  
Equinatoxin Ii ◽  
Denatured States ◽  
Uv Melting

scholarly journals UV Melting and Footprinting Studies of Three Structures of an RNA Aptamer with the Same Sequence

2009 ◽  
Vol 96 (3) ◽  
pp. 579a
Author(s):  
Sabarinath Jayaseelan ◽  
Zhen Huang ◽  
Carla Theimer ◽  
Li Niu
Keyword(s):  
Rna Aptamer ◽  
Uv Melting

scholarly journals Synthesis of 2′-O-methyl-RNAs incorporating a 3-deazaguanine, and UV melting and computational studies on its hybridization properties

2006 ◽  
Vol 34 (16) ◽  
pp. 4324-4334 ◽  
Author(s):  
Kohji Seio ◽  
Takeshi Sasami ◽  
Ryuya Tawarada ◽  
Mitsuo Sekine
Keyword(s):  
Computational Studies ◽  
Uv Melting

scholarly journals An Influence of Modification with Phosphoryl Guanidine Combined with a 2′-O-Methyl or 2′-Fluoro Group on the Small-Interfering-RNA Effect

2021 ◽  
Vol 22 (18) ◽  
pp. 9784
Author(s):  
Anna S. Pavlova ◽  
Kristina I. Yakovleva ◽  
Anna V. Epanchitseva ◽  
Maxim S. Kupryushkin ◽  
Inna A. Pyshnaya ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Fluorescent Protein ◽  
Cultured Cells ◽  
Biochemical Properties ◽  
Rnase A ◽  
Snake Venom Phosphodiesterase ◽  
Passenger Strand ◽  
Green Fluorescent ◽  
Uv Melting ◽  
Interfering Rna

Small interfering RNA (siRNA) is the most important tool for the manipulation of mRNA expression and needs protection from intracellular nucleases when delivered into the cell. In this work, we examined the effects of siRNA modification with the phosphoryl guanidine (PG) group, which, as shown earlier, makes oligodeoxynucleotides resistant to snake venom phosphodiesterase. We obtained a set of siRNAs containing combined modifications PG/2′-O-methyl (2′-OMe) or PG/2′-fluoro (2′-F); biophysical and biochemical properties were characterized for each duplex. We used the UV-melting approach to estimate the thermostability of the duplexes and RNAse A degradation assays to determine their stability. The ability to induce silencing was tested in cultured cells stably expressing green fluorescent protein. The introduction of the PG group as a rule decreased the thermodynamic stability of siRNA. At the same time, the siRNAs carrying PG groups showed increased resistance to RNase A. A gene silencing experiment indicated that the PG-modified siRNA retained its activity if the modifications were introduced into the passenger strand.

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