scholarly journals Unraveling the Kinetics of Spare-Tire DNA G-Quadruplex Folding

2021 ◽  
Vol 143 (16) ◽  
pp. 6185-6193
Author(s):  
J. Tassilo Grün ◽  
Anja Blümler ◽  
Ines Burkhart ◽  
Julia Wirmer-Bartoschek ◽  
Alexander Heckel ◽  
...  
Keyword(s):  
G Quadruplex ◽  
Kinetics Of

scholarly journals Folding Kinetics of Multiple G-Quadruplex Telomeric DNA Structures

2020 ◽  
Vol 118 (3) ◽  
pp. 335a
Author(s):  
Emil L. Kristoffersen ◽  
Andrea Coletta ◽  
Line Lund ◽  
Birgit Schiøtt ◽  
Victoria Birkedal
Keyword(s):  
Folding Kinetics ◽  
Telomeric Dna ◽  
Dna Structures ◽  
G Quadruplex ◽  
Kinetics Of

scholarly journals Folding Kinetics of G-Quadruplexes: Duplex Stem Loops Drive and Accelerate G-Quadruplex Folding

2020 ◽  
Vol 124 (25) ◽  
pp. 5122-5130 ◽  
Author(s):  
Thi Quynh Ngoc Nguyen ◽  
Kah Wai Lim ◽  
Anh Tuân Phan
Keyword(s):  
Folding Kinetics ◽  
G Quadruplex ◽  
Kinetics Of

Ultrafast Microfluidic Mixer for Tracking the Early Folding Kinetics of Human Telomere G-Quadruplex

2014 ◽  
Vol 86 (9) ◽  
pp. 4333-4339 ◽  
Author(s):  
Ying Li ◽  
Chao Liu ◽  
Xiaojun Feng ◽  
Youzhi Xu ◽  
Bi-Feng Liu
Keyword(s):  
Folding Kinetics ◽  
Microfluidic Mixer ◽  
G Quadruplex ◽  
Human Telomere ◽  
Kinetics Of

scholarly journals Stability and kinetics of G-quadruplex structures

2008 ◽  
Vol 36 (17) ◽  
pp. 5482-5515 ◽  
Author(s):  
A. N. Lane ◽  
J. B. Chaires ◽  
R. D. Gray ◽  
J. O. Trent
Keyword(s):  
G Quadruplex ◽  
Kinetics Of

scholarly journals Folding Kinetics of Single Human Telomeric G-Quadruplex Affected by Cisplatin

ACS Omega ◽  
2016 ◽  
Vol 1 (2) ◽  
pp. 244-250 ◽  
Author(s):  
Hai-Peng Ju ◽  
Yi-Zhou Wang ◽  
Jing You ◽  
Xi-Miao Hou ◽  
Xu-Guang Xi ◽  
...  
Keyword(s):  
Folding Kinetics ◽  
G Quadruplex ◽  
Kinetics Of

scholarly journals Effects of monovalent cations on folding kinetics of G-quadruplexes

2017 ◽  
Vol 37 (4) ◽  
Author(s):  
Jing You ◽  
Hui Li ◽  
Xi-Ming Lu ◽  
Wei Li ◽  
Peng-Ye Wang ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Genome Stability ◽  
Structural Transition ◽  
Monovalent Cation ◽  
Monovalent Cations ◽  
Folding Kinetics ◽  
Folding Rates ◽  
Type K ◽  
G Quadruplex ◽  
Kinetics Of

G-quadruplexes are special structures existing at the ends of human telomeres, the folding kinetics of which are essential for their functions, such as in the maintenance of genome stability and the protection of chromosome ends. In the present study, we investigated the folding kinetics of G-quadruplex in different monovalent cation environments and determined the detailed kinetic parameters for Na+- and K+-induced G-quadruplex folding, and for its structural transition from the basket-type Na+ form to the hybrid-type K+ form. More interestingly, although Li+ was often used in previous studies of G-quadruplex folding as a control ion supposed to have no effect, we have found that Li+ can actually influence the folding kinetics of both Na+- and K+-induced G-quadruplexes significantly and in different ways, by changing the folding fraction of Na+-induced G-quadruplexes and greatly increasing the folding rates of K+-induced G-quadruplexes. The present study may shed new light on the roles of monovalent cations in G-quadruplex folding and should be useful for further studies of the underlying folding mechanism.

scholarly journals Monitoring G-quadruplex formation with DNA carriers and solid-state nanopores

2019 ◽  
Author(s):  
Filip Bošković ◽  
Jinbo Zhu ◽  
Kaikai Chen ◽  
Ulrich F. Keyser
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Drug Targets ◽  
Gene Expression Regulation ◽  
Single Stranded Dna ◽  
Single Molecule Level ◽  
G Quadruplex ◽  
Human Viruses ◽  
Kinetics Of ◽  
Dna Carriers

ABSTRACTG-quadruplexes (Gq) are guanine-rich DNA structures formed by single-stranded DNA. They are of paramount significance to gene expression regulation, but also drug targets for cancer and human viruses. Current ensemble and single-molecule methods require fluorescent labels, which can affect Gq folding kinetics. Here we introduce, a single-molecule Gq nanopore assay (smGNA) to detect Gqs and kinetics of Gq formation. We use ~5 nm solid-state nanopores to detect various Gq structural variants attached to designed DNA carriers. Gqs can be identified by localizing their positions along designed DNA carriers establishing smGNA as a tool for Gq mapping. In addition, smGNA allows for discrimination of (un-)folded Gq structures, provides insights into single-molecule kinetics of G-quadruplex folding, and probes quadruplex-to-duplex structural transitions. smGNA can elucidate the formation of G-quadruplexes at the single-molecule level without labelling and has potential implications on the study of these structures both in single-stranded DNA and in genomic samples.

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