Long-Range Distance Measurements to the Phosphodiester Backbone of Solid Nucleic Acids Using31P−19F REDOR NMR

1999 ◽  
Vol 121 (25) ◽  
pp. 6070-6071 ◽  
Author(s):  
Matthew E. Merritt ◽  
Snorri Th. Sigurdsson ◽  
Gary P. Drobny
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Distance Measurements ◽  
Phosphodiester Backbone

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

2011 ◽  
Vol 50 (22) ◽  
pp. 5070-5074 ◽  
Author(s):  
Ivan Krstić ◽  
Robert Hänsel ◽  
Olga Romainczyk ◽  
Joachim W. Engels ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
In Cells

Long-Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

2011 ◽  
Vol 123 (22) ◽  
pp. 5176-5180 ◽  
Author(s):  
Ivan Krstić ◽  
Robert Hänsel ◽  
Olga Romainczyk ◽  
Joachim W. Engels ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
In Cells

Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded β-Peptides

2019 ◽  
Vol 25 (9) ◽  
pp. 2203-2207 ◽  
Author(s):  
Janine Wegner ◽  
Gabriele Valora ◽  
Karin Halbmair ◽  
Annemarie Kehl ◽  
Brigitte Worbs ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Long Range ◽  
Spin Label ◽  
Distance Measurements

Efficient localization of a native metal ion within a protein by Cu2+-based EPR distance measurements

2019 ◽  
Vol 21 (20) ◽  
pp. 10238-10243 ◽  
Author(s):  
Austin Gamble Jarvi ◽  
Timothy F. Cunningham ◽  
Sunil Saxena
Keyword(s):  
Binding Site ◽  
Long Range ◽  
Metal Binding ◽  
Metal Ion ◽  
Native Metal ◽  
Distance Measurements ◽  
Metal Binding Site ◽  
Paramagnetic Metal

A native paramagnetic metal binding site in a protein is located with less than 2 Å resolution by a combination of double histidine (dHis) based Cu2+ labeling and long range distance measurements by EPR.

scholarly journals Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution

2020 ◽  
Vol 48 (9) ◽  
pp. e49-e49 ◽  
Author(s):  
Shreya Ghosh ◽  
Matthew J Lawless ◽  
Hanna J Brubaker ◽  
Kevin Singewald ◽  
Michael R Kurpiewski ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Conformational Changes ◽  
Continuous Wave ◽  
Md Simulations ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Pulsed Epr ◽  
Distance Distributions ◽  
Dna Backbone ◽  
Double Electron Electron Resonance

Abstract Electron paramagnetic resonance (EPR) has become an important tool to probe conformational changes in nucleic acids. An array of EPR labels for nucleic acids are available, but they often come at the cost of long tethers, are dependent on the presence of a particular nucleotide or can be placed only at the termini. Site directed incorporation of Cu2+-chelated to a ligand, 2,2′dipicolylamine (DPA) is potentially an attractive strategy for site-specific, nucleotide independent Cu2+-labelling in DNA. To fully understand the potential of this label, we undertook a systematic and detailed analysis of the Cu2+-DPA motif using EPR and molecular dynamics (MD) simulations. We used continuous wave EPR experiments to characterize Cu2+ binding to DPA as well as optimize Cu2+ loading conditions. We performed double electron-electron resonance (DEER) experiments at two frequencies to elucidate orientational selectivity effects. Furthermore, comparison of DEER and MD simulated distance distributions reveal a remarkable agreement in the most probable distances. The results illustrate the efficacy of the Cu2+-DPA in reporting on DNA backbone conformations for sufficiently long base pair separations. This labelling strategy can serve as an important tool for probing conformational changes in DNA upon interaction with other macromolecules.

scholarly journals High-resolution measurement of long-range distances in RNA: pulse EPR spectroscopy with TEMPO-labeled nucleotides

2016 ◽  
Vol 7 (5) ◽  
pp. 3172-3180 ◽  
Author(s):  
Karin Halbmair ◽  
Jan Seikowski ◽  
Igor Tkach ◽  
Claudia Höbartner ◽  
Deniz Sezer ◽  
...  
Keyword(s):  
High Resolution ◽  
Long Range ◽  
Epr Spectroscopy ◽  
Base Pair ◽  
Distance Measurements ◽  
Model Free ◽  
High Resolution Measurement ◽  
Resolution Measurement ◽  
Pair Separation ◽  
Pulse Epr

Distance measurements in RNAs by pulse EPR with TEMPO-labeled nucleotides allow for model free conversion of distances into base-pair separation.

Rapid and precise absolute distance measurements at long range

2009 ◽  
Vol 3 (6) ◽  
pp. 351-356 ◽  
Author(s):  
I. Coddington ◽  
W. C. Swann ◽  
L. Nenadovic ◽  
N. R. Newbury
Keyword(s):  
Long Range ◽  
Absolute Distance ◽  
Distance Measurements

Distance measurements in nucleic acids using windowless dipolar recoupling solid state NMR

1996 ◽  
Vol 7 (3) ◽  
pp. 211-228 ◽  
Author(s):  
M.A. Mehta ◽  
D.M. Gregory ◽  
S. Kiihne ◽  
D.J. Mitchell ◽  
M.E. Hatcher ◽  
...  
Keyword(s):  
Solid State ◽  
Nucleic Acids ◽  
Solid State Nmr ◽  
Distance Measurements ◽  
Dipolar Recoupling
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