Synthesis and Spectroscopic Properties of a Prototype Single Molecule Dual Imaging Agent Comprising a Heterobimetallic Rhenium−Gadolinium Complex

2008 ◽  
Vol 130 (7) ◽  
pp. 2178-2179 ◽  
Author(s):  
Thelma Koullourou ◽  
Louise S. Natrajan ◽  
Hiren Bhavsar ◽  
Pope ◽  
Jianghua Feng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Spectroscopic Properties ◽  
Imaging Agent ◽  
Gadolinium Complex ◽  
Dual Imaging

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Supramolecular polymer of Schiff base gadolinium complex: Synthesis, crystal structure and spectroscopic properties

2015 ◽  
Vol 430 ◽  
pp. 108-113 ◽  
Author(s):  
Małgorzata T. Kaczmarek ◽  
Renata Jastrząb ◽  
Maciej Kubicki ◽  
Mateusz Gierszewski ◽  
Marek Sikorski
Keyword(s):  
Crystal Structure ◽  
Schiff Base ◽  
Spectroscopic Properties ◽  
Supramolecular Polymer ◽  
Gadolinium Complex

scholarly journals Mixed lanthanide oxide nanoparticles as dual imaging agent in biomedicine

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Wenlong Xu ◽  
Badrul Alam Bony ◽  
Cho Rong Kim ◽  
Jong Su Baeck ◽  
Yongmin Chang ◽  
...  
Keyword(s):  
Imaging Agent ◽  
Oxide Nanoparticles ◽  
Lanthanide Oxide ◽  
Dual Imaging

Design and Synthesis of Fluorinated Amphiphile as 19F MRI/Fluorescence Dual-Imaging Agent by Tuning the Self-Assembly

2015 ◽  
Vol 80 (12) ◽  
pp. 6360-6366 ◽  
Author(s):  
Shaowei Bo ◽  
Cong Song ◽  
Yu Li ◽  
Weijiang Yu ◽  
Shizhen Chen ◽  
...  
Keyword(s):  
Self Assembly ◽  
The Self ◽  
Imaging Agent ◽  
Design And Synthesis ◽  
19F Mri ◽  
Dual Imaging

scholarly journals Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series

2020 ◽  
Author(s):  
Christian Gebhardt ◽  
Martin Lehmann ◽  
Maria M. Reif ◽  
Martin Zacharias ◽  
Thorben Cordes
Keyword(s):  
Single Molecule ◽  
Continuous Wave ◽  
Structural Similarity ◽  
Spectroscopic Characterization ◽  
Spectroscopic Properties ◽  
Molecular Structures ◽  
Attractive Alternative ◽  
Alexa Fluor ◽  
Chemical Structures ◽  
Research Fields

AbstractThe use of fluorescence techniques has had an enormous impact on various research fields including imaging, biochemical assays, DNA-sequencing and medical technologies. This has been facilitated by the availability of numerous commercial dyes, but often information about the chemical structures of dyes (and their linkers) are a well-kept secret. This can lead to problems for applications where a knowledge of the dye structure is necessary to predict (unwanted) dye-target interactions, or to establish structural models of the dye-target complex. Using a combination of spectroscopy, mass spectrometry and molecular dynamics simulations, we here investigate the molecular structures and spectroscopic properties of dyes from the Alexa Fluor (Alexa Fluor 555 and 647) and AF series (AF555, AF647, AFD647). Based on available data and published structures of the AF and Cy dyes, we present two possible structures for Alexa Fluor 555. We also resolve conflicting reports on the linker composition of Alexa Fluor 647. A comprehensive comparison between Alexa Fluor and AF dyes by continuous-wave absorption and emission spectroscopy, quantum yield determination, fluorescence lifetime and anisotropy spectroscopy of free and protein-attached dyes, supports the suggestion that the Alexa Fluor and AF dyes have a high degree of structural similarity. In addition, we compared Alexa Fluor 555 and Alexa Fluor 647 to their structural homologs AF555 and AF(D)647 in single-molecule FRET applications. Both pairs showed excellent performance in solution-based smFRET experiments using alternating laser excitation demonstrating that the AF-fluorophores are an attractive alternative to Alexa- and Cy-dyes for smFRET studies, and suggesting their usefulness for other fluorescence applications.

Ultrafine PEG-capped gadolinia nanoparticles: cytotoxicity and potential biomedical applications for MRI and luminescent imaging

RSC Advances ◽  
2014 ◽  
Vol 4 (65) ◽  
pp. 34343-34349 ◽  
Author(s):  
Timur Sh. Atabaev ◽  
Jong Ho Lee ◽  
Dong-Wook Han ◽  
Hyung-Kook Kim ◽  
Yoon-Hwae Hwang
Keyword(s):  
Optical Imaging ◽  
Biomedical Applications ◽  
Gadolinium Oxide ◽  
Imaging Agent ◽  
Quick Method ◽  
Erbium Ions ◽  
Dual Imaging

Ultrafine PEG-capped gadolinium oxide NPs doped with erbium ions, which could serve as a dual-imaging agent for MRI/optical imaging were synthesized using a simple, green, and quick method.

scholarly journals Spectroscopic properties of photosystem II core complexes from Thermosynechococcus elongatus revealed by single-molecule experiments

2014 ◽  
Vol 1837 (6) ◽  
pp. 773-781 ◽  
Author(s):  
Marc Brecht ◽  
Sepideh Skandary ◽  
Julia Hellmich ◽  
Carina Glöckner ◽  
Alexander Konrad ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Single Molecule ◽  
Spectroscopic Properties

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Rhodamine Derivative ◽  
New Type ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

AbstractWe present a new type of photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent isomerization followed by protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we created probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

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