scholarly journals Spectroscopic properties of photosystem II core complexes from Thermosynechococcus elongatus revealed by single-molecule experiments

2014 ◽  
Vol 1837 (6) ◽  
pp. 773-781 ◽  
Author(s):  
Marc Brecht ◽  
Sepideh Skandary ◽  
Julia Hellmich ◽  
Carina Glöckner ◽  
Alexander Konrad ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Single Molecule ◽  
Spectroscopic Properties

Synthesis and Spectroscopic Properties of a Prototype Single Molecule Dual Imaging Agent Comprising a Heterobimetallic Rhenium−Gadolinium Complex

2008 ◽  
Vol 130 (7) ◽  
pp. 2178-2179 ◽  
Author(s):  
Thelma Koullourou ◽  
Louise S. Natrajan ◽  
Hiren Bhavsar ◽  
Pope ◽  
Jianghua Feng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Spectroscopic Properties ◽  
Imaging Agent ◽  
Gadolinium Complex ◽  
Dual Imaging

Two Interconvertible Structures that Explain the Spectroscopic Properties of the Oxygen-Evolving Complex of Photosystem II in the S2State

2012 ◽  
Vol 51 (39) ◽  
pp. 9935-9940 ◽  
Author(s):  
Dimitrios A. Pantazis ◽  
William Ames ◽  
Nicholas Cox ◽  
Wolfgang Lubitz ◽  
Frank Neese
Keyword(s):  
Photosystem Ii ◽  
Spectroscopic Properties ◽  
Oxygen Evolving Complex ◽  
Oxygen Evolving

Surface and Spectroscopic Properties of Photosystem II Core Complex at the Nitrogen/Water Interface

Langmuir ◽  
1998 ◽  
Vol 14 (14) ◽  
pp. 3954-3963 ◽  
Author(s):  
Judith Gallant ◽  
Hugo Lavoie ◽  
Alain Tessier ◽  
Gaétan Munger ◽  
Roger M. Leblanc ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Spectroscopic Properties ◽  
Water Interface ◽  
Core Complex

scholarly journals Spectroscopic Properties of Reaction Center Pigments in Photosystem II Core Complexes: Revision of the Multimer Model

2008 ◽  
Vol 95 (1) ◽  
pp. 105-119 ◽  
Author(s):  
Grzegorz Raszewski ◽  
Bruce A. Diner ◽  
Eberhard Schlodder ◽  
Thomas Renger
Keyword(s):  
Photosystem Ii ◽  
Reaction Center ◽  
Spectroscopic Properties

scholarly journals Kinetic and Spectroscopic Properties of the YZ•Radical in Ca2+- and Cl--Depleted Photosystem II Preparations

Biochemistry ◽  
1998 ◽  
Vol 37 (24) ◽  
pp. 8816-8816 ◽  
Author(s):  
Nikos Lydakis-Simantiris ◽  
Pierre Dorlet ◽  
Demetrios F. Ghanotakis ◽  
Gerald T. Babcock
Keyword(s):  
Photosystem Ii ◽  
Spectroscopic Properties

scholarly journals Photoactivation of silicon rhodamines via a light-induced protonation

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Michelle S. Frei ◽  
Philipp Hoess ◽  
Marko Lampe ◽  
Bianca Nijmeijer ◽  
Moritz Kueblbeck ◽  
...  
Keyword(s):  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Super Resolution ◽  
Spectroscopic Properties ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Localization Microscopy ◽  
Super Resolution Microscopy ◽  
Single Molecule Localization Microscopy

Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Kinetic and Spectroscopic Properties of the YZ•Radical in Ca2+- and Cl--Depleted Photosystem II Preparations†

Biochemistry ◽  
1998 ◽  
Vol 37 (18) ◽  
pp. 6427-6435 ◽  
Author(s):  
Nikos Lydakis-Simantiris ◽  
Pierre Dorlet ◽  
Demetrios F. Ghanotakis ◽  
Gerald T. Babcock
Keyword(s):  
Photosystem Ii ◽  
Spectroscopic Properties

scholarly journals Molecular and spectroscopic characterization of green and red cyanine fluorophores from the Alexa Fluor and AF series

2020 ◽  
Author(s):  
Christian Gebhardt ◽  
Martin Lehmann ◽  
Maria M. Reif ◽  
Martin Zacharias ◽  
Thorben Cordes
Keyword(s):  
Single Molecule ◽  
Continuous Wave ◽  
Structural Similarity ◽  
Spectroscopic Characterization ◽  
Spectroscopic Properties ◽  
Molecular Structures ◽  
Attractive Alternative ◽  
Alexa Fluor ◽  
Chemical Structures ◽  
Research Fields

AbstractThe use of fluorescence techniques has had an enormous impact on various research fields including imaging, biochemical assays, DNA-sequencing and medical technologies. This has been facilitated by the availability of numerous commercial dyes, but often information about the chemical structures of dyes (and their linkers) are a well-kept secret. This can lead to problems for applications where a knowledge of the dye structure is necessary to predict (unwanted) dye-target interactions, or to establish structural models of the dye-target complex. Using a combination of spectroscopy, mass spectrometry and molecular dynamics simulations, we here investigate the molecular structures and spectroscopic properties of dyes from the Alexa Fluor (Alexa Fluor 555 and 647) and AF series (AF555, AF647, AFD647). Based on available data and published structures of the AF and Cy dyes, we present two possible structures for Alexa Fluor 555. We also resolve conflicting reports on the linker composition of Alexa Fluor 647. A comprehensive comparison between Alexa Fluor and AF dyes by continuous-wave absorption and emission spectroscopy, quantum yield determination, fluorescence lifetime and anisotropy spectroscopy of free and protein-attached dyes, supports the suggestion that the Alexa Fluor and AF dyes have a high degree of structural similarity. In addition, we compared Alexa Fluor 555 and Alexa Fluor 647 to their structural homologs AF555 and AF(D)647 in single-molecule FRET applications. Both pairs showed excellent performance in solution-based smFRET experiments using alternating laser excitation demonstrating that the AF-fluorophores are an attractive alternative to Alexa- and Cy-dyes for smFRET studies, and suggesting their usefulness for other fluorescence applications.

scholarly journals Variation of Exciton-Vibrational Coupling in Photosystem II Core Complexes from Thermosynechococcus elongatus As Revealed by Single-Molecule Spectroscopy

2015 ◽  
Vol 119 (11) ◽  
pp. 4203-4210 ◽  
Author(s):  
Sepideh Skandary ◽  
Martin Hussels ◽  
Alexander Konrad ◽  
Thomas Renger ◽  
Frank Müh ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Vibrational Coupling
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