Simultaneous Orthogonal Dual-Click Approach to Tough, in-Situ-Forming Hydrogels for Cell Encapsulation

2015 ◽  
Vol 137 (4) ◽  
pp. 1618-1622 ◽  
Author(s):  
Vinh X. Truong ◽  
Matthew P. Ablett ◽  
Stephen M. Richardson ◽  
Judith A. Hoyland ◽  
Andrew P. Dove
Keyword(s):  
Cell Encapsulation ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

scholarly journals Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

2020 ◽  
Author(s):  
Isabel Pereira ◽  
José Eduardo Pereira ◽  
Luís Maltez ◽  
Alexandra Rodrigues ◽  
Catarina Rodrigues ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Substitute ◽  
Bone Substitutes ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Micro Computed Tomography ◽  
Synthetic Bone Substitute ◽  
In Situ Forming ◽  
Bioactive Agents ◽  
In Situ Forming Hydrogels

Abstract The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike®) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike® granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.

Catechol-modified hyaluronic acid: in situ-forming hydrogels by auto-oxidation of catechol or photo-oxidation using visible light

2017 ◽  
Vol 74 (10) ◽  
pp. 4069-4085 ◽  
Author(s):  
Takeshi Sato ◽  
Takao Aoyagi ◽  
Mitsuhiro Ebara ◽  
Rachel Auzély-Velty
Keyword(s):  
Hyaluronic Acid ◽  
Visible Light ◽  
Photo Oxidation ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

Moist-Retaining, Self-Recoverable, Bioadhesive, and Transparent in Situ Forming Hydrogels To Accelerate Wound Healing

2020 ◽  
Vol 12 (2) ◽  
pp. 2023-2038 ◽  
Author(s):  
Jun Li ◽  
Fan Yu ◽  
Gong Chen ◽  
Jia Liu ◽  
Xiao-Long Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Accelerate Wound Healing ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

In situ forming hydrogels of hyaluronic acid and inulin derivatives for cartilage regeneration

2015 ◽  
Vol 122 ◽  
pp. 408-416 ◽  
Author(s):  
Fabio S. Palumbo ◽  
Calogero Fiorica ◽  
Mauro Di Stefano ◽  
Giovanna Pitarresi ◽  
Alessandro Gulino ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cartilage Regeneration ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

Introduction to In Situ Forming Hydrogels for Biomedical Applications

2014 ◽  
pp. 5-35 ◽  
Author(s):  
Bogyu Choi ◽  
Xian Jun Loh ◽  
Aloysius Tan ◽  
Chun Keat Loh ◽  
Enyi Ye ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
In Situ Forming ◽  
In Situ Forming Hydrogels

scholarly journals Characterization and Optimization of PLA Stereocomplexed Hydrogels for Local Gene Delivery Systems

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 796 ◽  
Author(s):  
Kwei-Yu Liu ◽  
Daniel G. Abebe ◽  
Elizabeth Rachel Wiley ◽  
Tomoko Fujiwara
Keyword(s):  
Gene Delivery ◽  
Degradation Process ◽  
Mechanistic Studies ◽  
Local Site ◽  
In Situ Forming ◽  
Copolymer Micelles ◽  
In Situ Forming Hydrogels ◽  
Acidic Degradation ◽  
Significant Factors

Localized gene delivery still remains as a challenging therapeutic method due to the multiple hurdles to overcome. One of the significant factors is a development of a matrix to carry and safely deliver genes at the local site in a controlled manner and then exit and disintegrate harmlessly. This report describes the structural and mechanistic studies on the in-situ forming hydrogels composed of the PEI/DNA multi-layered micelles to apply for gene therapy. The stereocomplexation-driven hydrogel systems from the DNA-loaded and DNA-free PLA-PEG-PLA triblock copolymer micelles that include enantiomeric polylactide blocks exhibited a sol-to-gel transitions between room and body temperatures. These hydrogels have well-described structure and compositions, and improved mechanical properties. Furthermore, the investigation of their degradation profiles and chemical analysis indicated the faster acidic degradation and stepwise degradation process of these micelle–hydrogel systems.

Sign in / Sign up

Export Citation Format

Share Document