Marked Improvement in Photoinduced Cell Death by a New Tris-heteroleptic Complex with Dual Action: Singlet Oxygen Sensitization and Ligand Dissociation

2014 ◽  
Vol 136 (49) ◽  
pp. 17095-17101 ◽  
Author(s):  
Bryan A. Albani ◽  
Bruno Peña ◽  
Nicholas A. Leed ◽  
Nataly A. B. G. de Paula ◽  
Christiane Pavani ◽  
...  
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Marked Improvement ◽  
Ligand Dissociation ◽  
Dual Action ◽  
Heteroleptic Complex

scholarly journals Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

2021 ◽  
Vol 22 (11) ◽  
pp. 5548
Author(s):  
Yan Li ◽  
Tianyu Tang ◽  
Haejune Lee ◽  
Kiwon Song
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Proliferative Effect ◽  
Huh7 Cells ◽  
Anti Cancer ◽  
Cold Atmospheric Pressure Plasma ◽  
Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

Execution of programmed cell death by singlet oxygen generated inside the chloroplasts of Arabidopsis thaliana

PROTOPLASMA ◽  
2020 ◽  
Vol 257 (3) ◽  
pp. 841-851 ◽  
Author(s):  
Vivek Ambastha ◽  
Garima Chauhan ◽  
Budhi Sagar Tiwari ◽  
Baishnab C Tripathy
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Singlet Oxygen

scholarly journals Implication of the oep16-1 Mutation in a flu-Independent, Singlet Oxygen-Regulated Cell Death Pathway in Arabidopsis thaliana

2010 ◽  
Vol 52 (1) ◽  
pp. 84-95 ◽  
Author(s):  
Iga Samol ◽  
Frank Buhr ◽  
Armin Springer ◽  
Stephan Pollmann ◽  
Abder Lahroussi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Cell Death Pathway ◽  
Regulated Cell Death

scholarly journals Green light-induced apoptosis in cancer cells by a tetrapyridyl ruthenium prodrug offering two trans coordination sites

2016 ◽  
Vol 7 (8) ◽  
pp. 4922-4929 ◽  
Author(s):  
V. H. S. van Rixel ◽  
B. Siewert ◽  
S. L. Hopkins ◽  
S. H. C. Askes ◽  
A. Busemann ◽  
...  
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Green Light ◽  
Oxygen Generation ◽  
Light Cell ◽  
Singlet Oxygen Generation ◽  
Coordination Sites ◽  
Induced Apoptosis ◽  
Apoptosis In Cancer Cells

In this work, two new photopharmacological ruthenium prodrugs are described that can be activated by green light. Cell death occurs via apoptosis; it is not a consequence of singlet oxygen generation, but of light-induced photosubstitution reactions.

Cancerous Cell Death from Sensitizer Free Photoactivation of Singlet Oxygen

2011 ◽  
Vol 88 (1) ◽  
pp. 167-174 ◽  
Author(s):  
François Anquez ◽  
Ikram El Yazidi-Belkoura ◽  
Stéphane Randoux ◽  
Pierre Suret ◽  
Emmanuel Courtade
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Cancerous Cell

Physical Plasma Elicits Immunogenic Cancer Cell Death and Mitochondrial Singlet Oxygen

2018 ◽  
Vol 2 (2) ◽  
pp. 138-146 ◽  
Author(s):  
Sander Bekeschus ◽  
Anne Mueller ◽  
Vandana Miller ◽  
Udo Gaipl ◽  
Klaus-Dieter Weltmann
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Cancer Cell ◽  
Cancer Cell Death ◽  
Physical Plasma

scholarly journals Singlet Oxygen-Induced Membrane Disruption and Serpin-Protease Balance in Vacuolar-Driven Cell Death

2016 ◽  
Vol 171 (3) ◽  
pp. 1616-1625 ◽  
Author(s):  
Eugene Koh ◽  
Raanan Carmieli ◽  
Avishai Mor ◽  
Robert Fluhr
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Membrane Disruption ◽  
Induced Membrane

scholarly journals Prostate-specific membrane antigen (PSMA) targeted singlet oxygen delivery via endoperoxide tethered ligands

2022 ◽  
Author(s):  
Lei Wang ◽  
Lei Tang ◽  
Yingjie Liu ◽  
Hao Wu ◽  
Ziang Liu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Oxygen Delivery ◽  
Modular Design ◽  
Prostate Specific Membrane Antigen ◽  
Prostate Cancer Cells ◽  
Tethered Ligands ◽  
Specific Membrane

A PSMA targeting ligand is functionalized with endoperoxides which thermally release singlet oxygen. The results show that this modular design results in significantly more cell death in PSMA-expressing prostate cancer cells.

Emerging Therapeutic Targets in Oncologic Photodynamic Therapy

2019 ◽  
Vol 24 (44) ◽  
pp. 5268-5295 ◽  
Author(s):  
Gina Manda ◽  
Mihail E. Hinescu ◽  
Ionela V. Neagoe ◽  
Luis F.V. Ferreira ◽  
Rica Boscencu ◽  
...  
Keyword(s):  
Cell Death ◽  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Molecular Mechanisms ◽  
Therapeutic Targets ◽  
Molecular Networks ◽  
Systems Medicine ◽  
Therapeutic Tools

Background:Reactive oxygen species sustain tumorigenesis and cancer progression through deregulated redox signalling which also sensitizes cancer cells to therapy. Photodynamic therapy (PDT) is a promising anti-cancer therapy based on a provoked singlet oxygen burst, exhibiting a better toxicological profile than chemo- and radiotherapy. Important gaps in the knowledge on underlining molecular mechanisms impede on its translation towards clinical applications.Aims and Methods:The main objective of this review is to critically analyse the knowledge lately gained on therapeutic targets related to redox and inflammatory networks underlining PDT and its outcome in terms of cell death and resistance to therapy. Emerging therapeutic targets and pharmaceutical tools will be documented based on the identified molecular background of PDT.Results:Cellular responses and molecular networks in cancer cells exposed to the PDT-triggered singlet oxygen burst and the associated stresses are analysed using a systems medicine approach, addressing both cell death and repair mechanisms. In the context of immunogenic cell death, therapeutic tools for boosting anti-tumor immunity will be outlined. Finally, the transcription factor NRF2, which is a major coordinator of cytoprotective responses, is presented as a promising pharmacologic target for developing co-therapies designed to increase PDT efficacy.Conclusion:There is an urgent need to perform in-depth molecular investigations in the field of PDT and to correlate them with clinical data through a systems medicine approach for highlighting the complex biological signature of PDT. This will definitely guide translation of PDT to clinic and the development of new therapeutic strategies aimed at improving PDT.

scholarly journals Influence of Polymer Charge on the Localization and Dark- and Photo-Induced Toxicity of a Potential Type I Photosensitizer in Cancer Cell Models

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1127 ◽  
Author(s):  
Mikael Lindgren ◽  
Odrun A. Gederaas ◽  
Monica Siksjø ◽  
Tom A. Hansen ◽  
Lena Chen ◽  
...  
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Deuterium Oxide ◽  
Current Trend ◽  
Chinese Hamster ◽  
Type I ◽  
Type Ii ◽  
Molecular Systems ◽  
Cell Compartments ◽  
Survival Studies

A current trend within photo-dynamic therapy (PDT) is the development of molecular systems targeting hypoxic tumors. Thus, type I PDT sensitizers could here overcome traditional type II molecular systems that rely on the photo-initiated production of toxic singlet oxygen. Here, we investigate the cell localization properties and toxicity of two polymeric anthracene-based fluorescent probes (neutral Ant-PHEA and cationic Ant-PIm). The cell death and DNA damage of Chinese hamster ovary cancer cells (CHO-K1) were characterized as combining PDT, cell survival studies (MTT-assay), and comet assay. Confocal microscopy was utilized on samples incubated together with either DRAQ5, Lyso Tracker Red, or Mito Tracker Deep Red in order to map the localization of the sensitizer into the nucleus and other cell compartments. While Ant-PHEA did not cause significant damage to the cell, Ant-PIm showed increased cell death upon illumination, at the cost of a significant dark toxicity. Both anthracene chromophores localized in cell compartments of the cytosol. Ant-PIm showed a markedly improved selectivity toward lysosomes and mitochondria, two important biological compartments for the cell’s survival. None of the two anthracene chromophores showed singlet oxygen formation upon excitation in solvents such as deuterium oxide or methanol. Conclusively, the significant photo-induced cell death that could be observed with Ant-PIm suggests a possible type I PDT mechanism rather than the usual type II mechanism.

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