scholarly journals Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

2014 ◽  
Vol 136 (32) ◽  
pp. 11308-11310 ◽  
Author(s):  
Rasmus Linser ◽  
Vladimir Gelev ◽  
Franz Hagn ◽  
Haribabu Arthanari ◽  
Sven G. Hyberts ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Free Expression ◽  
Methyl Labeling ◽  
Eukaryotic Membrane Proteins

Regulation of the water channel aquaporin-2 by posttranslational modification

2011 ◽  
Vol 300 (5) ◽  
pp. F1062-F1073 ◽  
Author(s):  
Hanne B. Moeller ◽  
Emma T. B. Olesen ◽  
Robert A. Fenton
Keyword(s):  
Membrane Proteins ◽  
Protein Interactions ◽  
Posttranslational Modifications ◽  
Posttranslational Modification ◽  
Water Channel ◽  
Cellular Functions ◽  
Aquaporin 2 ◽  
Eukaryotic Membrane Proteins ◽  
Insight Into

The cellular functions of many eukaryotic membrane proteins, including the vasopressin-regulated water channel aquaporin-2 (AQP2), are regulated by posttranslational modifications. In this article, we discuss the experimental discoveries that have advanced our understanding of how posttranslational modifications affect AQP2 function, especially as they relate to the role of AQP2 in the kidney. We review the most recent data demonstrating that glycosylation and, in particular, phosphorylation and ubiquitination are mechanisms that regulate AQP2 activity, subcellular sorting and distribution, degradation, and protein interactions. From a clinical perspective, posttranslational modification resulting in protein misrouting or degradation may explain certain forms of nephrogenic diabetes insipidus. In addition to providing major insight into the function and dynamics of renal AQP2 regulation, the analysis of AQP2 posttranslational modification may provide general clues as to the role of posttranslational modification for regulation of other membrane proteins.

Insight into factors directing high production of eukaryotic membrane proteins; production of 13 human AQPs inPichia pastoris

2009 ◽  
Vol 26 (4) ◽  
pp. 215-227 ◽  
Author(s):  
Fredrik Öberg ◽  
Mikael Ekvall ◽  
Maria Nyblom ◽  
Fredrik Öberg ◽  
Mikael Ekvall ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
High Production ◽  
Eukaryotic Membrane Proteins ◽  
Insight Into

scholarly journals MemPype: a pipeline for the annotation of eukaryotic membrane proteins

2011 ◽  
Vol 39 (suppl) ◽  
pp. W375-W380 ◽  
Author(s):  
A. Pierleoni ◽  
V. Indio ◽  
C. Savojardo ◽  
P. Fariselli ◽  
P. L. Martelli ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Eukaryotic Membrane Proteins

Cell-free expression profiling of E. coli inner membrane proteins

PROTEOMICS ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 1762-1779 ◽  
Author(s):  
Daniel Schwarz ◽  
Daniel Daley ◽  
Tobias Beckhaus ◽  
Volker Dötsch ◽  
Frank Bernhard
Keyword(s):  
Membrane Proteins ◽  
Expression Profiling ◽  
Free Expression ◽  
Inner Membrane ◽  
E Coli

scholarly journals GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

2008 ◽  
Vol 3 (5) ◽  
pp. 784-798 ◽  
Author(s):  
David Drew ◽  
Simon Newstead ◽  
Yo Sonoda ◽  
Hyun Kim ◽  
Gunnar von Heijne ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Proteins ◽  
Optimization Scheme ◽  
Eukaryotic Membrane Proteins
Sign in / Sign up

Export Citation Format

Share Document