scholarly journals Native Chemical Ligation at Asx-Cys, Glx-Cys: Chemical Synthesis and High-Resolution X-ray Structure of ShK Toxin by Racemic Protein Crystallography

2013 ◽  
Vol 135 (32) ◽  
pp. 11911-11919 ◽  
Author(s):  
Bobo Dang ◽  
Tomoya Kubota ◽  
Kalyaneswar Mandal ◽  
Francisco Bezanilla ◽  
Stephen B. H. Kent
Keyword(s):  
High Resolution ◽  
Chemical Synthesis ◽  
Protein Crystallography ◽  
Native Chemical Ligation ◽  
Chemical Ligation ◽  
X Ray

Total Chemical Synthesis of Aureocin A53, Lacticin Q and Their Enantiomeric Counterparts

2020 ◽  
Author(s):  
Alexander J. Lander ◽  
Xuefei Li ◽  
Yi Jin ◽  
Louis YP Luk
Keyword(s):  
Crystal Structure ◽  
Chemical Synthesis ◽  
Native Chemical Ligation ◽  
Chemical Ligation ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Spectrum Activity ◽  
Synthetic Routes ◽  
Nanomolar Range ◽  
Broad Spectrum Activity

<div> <p><a>Aureocin A53 (AucA) and lacticin Q (LnqQ) are class IId bacteriocins that display broad-spectrum activity against Gram-positive bacteria in the nanomolar range, of which their modes of action is unclear and their synthesis has not been reported. Here, we reported the chemical synthetic routes of AucA and LnqQ, enabled through the technique of native chemical ligation. To demonstrate the versatility of the syntheses, we prepared their enantiomeric counterparts, </a>D-AucA and D-LnqQ, comprised of entirely D-amino acids. X-ray crystal structure of AucA obtained through racemic protein crystallography at 1.13 Å indicates unique Lys-Trp-sulfate network. This work lays foundations for gaining further mechanistic insights into these potent bacteriocins through total chemical synthesis. </p> </div> <br>

Total Chemical Synthesis of Aureocin A53, Lacticin Q and Their Enantiomeric Counterparts

2020 ◽  
Author(s):  
Alexander J. Lander ◽  
Xuefei Li ◽  
Yi Jin ◽  
Louis YP Luk
Keyword(s):  
Crystal Structure ◽  
Chemical Synthesis ◽  
Native Chemical Ligation ◽  
Chemical Ligation ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Spectrum Activity ◽  
Synthetic Routes ◽  
Nanomolar Range ◽  
Broad Spectrum Activity

<div> <p><a>Aureocin A53 (AucA) and lacticin Q (LnqQ) are class IId bacteriocins that display broad-spectrum activity against Gram-positive bacteria in the nanomolar range, of which their modes of action is unclear and their synthesis has not been reported. Here, we reported the chemical synthetic routes of AucA and LnqQ, enabled through the technique of native chemical ligation. To demonstrate the versatility of the syntheses, we prepared their enantiomeric counterparts, </a>D-AucA and D-LnqQ, comprised of entirely D-amino acids. X-ray crystal structure of AucA obtained through racemic protein crystallography at 1.13 Å indicates unique Lys-Trp-sulfate network. This work lays foundations for gaining further mechanistic insights into these potent bacteriocins through total chemical synthesis. </p> </div> <br>

scholarly journals Fully Convergent Chemical Synthesis of Ester Insulin: Determination of the High Resolution X-ray Structure by Racemic Protein Crystallography

2013 ◽  
Vol 135 (8) ◽  
pp. 3173-3185 ◽  
Author(s):  
Michal Avital-Shmilovici ◽  
Kalyaneswar Mandal ◽  
Zachary P. Gates ◽  
Nelson B. Phillips ◽  
Michael A. Weiss ◽  
...  
Keyword(s):  
High Resolution ◽  
Chemical Synthesis ◽  
Protein Crystallography ◽  
X Ray

One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

2014 ◽  
Vol 50 (44) ◽  
pp. 5837-5839 ◽  
Author(s):  
Man Pan ◽  
Yao He ◽  
Ming Wen ◽  
Fangming Wu ◽  
Demeng Sun ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Snake Venom ◽  
Structure Determination ◽  
Native Chemical Ligation ◽  
One Pot ◽  
Chemical Ligation ◽  
Venom Toxin ◽  
Snake Venom Toxin ◽  
Switch Strategy

An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation.

scholarly journals A Straightforward Methodology to Overcome Solubility Challenges for N-Terminal Cysteinyl Peptide Segments Used in Native Chemical Ligation

2020 ◽  
Author(s):  
Skander Abboud ◽  
El hadji Cisse ◽  
Michel Doudeau ◽  
Hélène Bénédetti ◽  
Vincent AUCAGNE
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Building Blocks ◽  
Native Chemical Ligation ◽  
Synthetic Approach ◽  
Chemical Ligation ◽  
Limited Solubility ◽  
Peptide Segments

One of the main limitations encountered during the chemical synthesis of proteins through native chemical ligation (NCL) is the limited solubility of some of the peptide segments. The most commonly used solution to overcome this problem is to derivatize the segment with a temporary solubilizing tag. Conveniently, the tag can be introduced on the thioester segment in such a way that it is removed concomitantly with the NCL reaction. We herein describe a generalization of this approach to N-terminal cysteinyl segment counterparts, using a straightforward synthetic approach that can be easily automated from commercially available building blocks, and applied it to a well-known problematic target, SUMO-2 (93 amino acids).

Facile and efficient chemical synthesis of APET×2, an ASIC-targeting toxin, via hydrazide-based native chemical ligation

Tetrahedron ◽  
2015 ◽  
Vol 71 (21) ◽  
pp. 3363-3366
Author(s):  
Si-Jian Li ◽  
Da-Liang Qu ◽  
Ye-Hai Wang ◽  
Yao He ◽  
Min Wen ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Native Chemical Ligation ◽  
Chemical Ligation

Chemical Synthesis of A Pore-Forming Antimicrobial Protein, Caenopore-5, by Using Native Chemical Ligation at a Glu-Cys Site

ChemBioChem ◽  
2014 ◽  
Vol 16 (2) ◽  
pp. 328-336 ◽  
Author(s):  
Karima Medini ◽  
Paul W. R. Harris ◽  
Kiel Hards ◽  
Andrew J. Dingley ◽  
Gregory M. Cook ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Native Chemical Ligation ◽  
Antimicrobial Protein ◽  
Chemical Ligation

scholarly journals Total chemical synthesis of human interferon alpha-2b via native chemical ligation

2015 ◽  
Vol 21 (7) ◽  
pp. 554-560 ◽  
Author(s):  
Jing Li ◽  
Clara Lehmann ◽  
Xishan Chen ◽  
Fabio Romerio ◽  
Wuyuan Lu
Keyword(s):  
Chemical Synthesis ◽  
Interferon Alpha ◽  
Native Chemical Ligation ◽  
Human Interferon ◽  
Chemical Ligation ◽  
Interferon Alpha 2B
Sign in / Sign up

Export Citation Format

Share Document