Specific Detection ofd-Glucose by a Tetraphenylethene-Based Fluorescent Sensor

2011 ◽  
Vol 133 (4) ◽  
pp. 660-663 ◽  
Author(s):  
Yi Liu ◽  
Chunmei Deng ◽  
Li Tang ◽  
Anjun Qin ◽  
Rongrong Hu ◽  
...  
Cell ◽  
2018 ◽  
Vol 174 (2) ◽  
pp. 481-496.e19 ◽  
Author(s):  
Fangmiao Sun ◽  
Jianzhi Zeng ◽  
Miao Jing ◽  
Jingheng Zhou ◽  
Jiesi Feng ◽  
...  

2019 ◽  
Vol 131 (5) ◽  
Author(s):  
Soham Samanta ◽  
Senjuti Halder ◽  
Utsab Manna ◽  
Gopal Das

2020 ◽  
Vol 49 (28) ◽  
pp. 9680-9687 ◽  
Author(s):  
Yong-Nian Zeng ◽  
He-Qi Zheng ◽  
Xing-Hao He ◽  
Gao-Juan Cao ◽  
Bin Wang ◽  
...  

A dual-emissive metal–organic framework was synthesized, and it represents the first MOF-based fluorescent ratiometric sensor for the turn-on sensing of hypochlorite with high efficiency and specificity.


RSC Advances ◽  
2013 ◽  
Vol 3 (34) ◽  
pp. 14543 ◽  
Author(s):  
Kai Sun ◽  
Xiaoli Liu ◽  
Yanyun Wang ◽  
Zhaoqiang Wu

2021 ◽  
Vol 12 ◽  
Author(s):  
Shihui Fan ◽  
Chao Ma ◽  
Xiaopeng Tian ◽  
Xiaoyi Ma ◽  
Mingcan Qin ◽  
...  

Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated “RFD-VV-M2” exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5–10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.


2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>


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