scholarly journals A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice

Cell ◽  
2018 ◽  
Vol 174 (2) ◽  
pp. 481-496.e19 ◽  
Author(s):  
Fangmiao Sun ◽  
Jianzhi Zeng ◽  
Miao Jing ◽  
Jingheng Zhou ◽  
Jiesi Feng ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Specific Detection

Specific Detection ofd-Glucose by a Tetraphenylethene-Based Fluorescent Sensor

2011 ◽  
Vol 133 (4) ◽  
pp. 660-663 ◽  
Author(s):  
Yi Liu ◽  
Chunmei Deng ◽  
Li Tang ◽  
Anjun Qin ◽  
Rongrong Hu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Specific Detection

scholarly journals Specific detection of hypochlorite: a cyanine based turn-on fluorescent sensor

2019 ◽  
Vol 131 (5) ◽  
Author(s):  
Soham Samanta ◽  
Senjuti Halder ◽  
Utsab Manna ◽  
Gopal Das
Keyword(s):  
Fluorescent Sensor ◽  
Specific Detection ◽  
Turn On

Dual-emissive metal–organic framework: a novel turn-on and ratiometric fluorescent sensor for highly efficient and specific detection of hypochlorite

2020 ◽  
Vol 49 (28) ◽  
pp. 9680-9687 ◽  
Author(s):  
Yong-Nian Zeng ◽  
He-Qi Zheng ◽  
Xing-Hao He ◽  
Gao-Juan Cao ◽  
Bin Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Fluorescent Sensor ◽  
Metal Organic Framework ◽  
Specific Detection ◽  
Turn On ◽  
Highly Efficient ◽  
Ratiometric Sensor ◽  
Metal Organic ◽  
Organic Framework

A dual-emissive metal–organic framework was synthesized, and it represents the first MOF-based fluorescent ratiometric sensor for the turn-on sensing of hypochlorite with high efficiency and specificity.

A polymer-based turn-on fluorescent sensor for specific detection of hydrogen sulfide

RSC Advances ◽  
2013 ◽  
Vol 3 (34) ◽  
pp. 14543 ◽  
Author(s):  
Kai Sun ◽  
Xiaoli Liu ◽  
Yanyun Wang ◽  
Zhaoqiang Wu
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Sensor ◽  
Specific Detection ◽  
Turn On

scholarly journals Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor

2021 ◽  
Vol 12 ◽  
Author(s):  
Shihui Fan ◽  
Chao Ma ◽  
Xiaopeng Tian ◽  
Xiaoyi Ma ◽  
Mingcan Qin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Vibrio Vulnificus ◽  
Detection Method ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Specific Detection ◽  
Aquatic Products ◽  
Specificity And Sensitivity ◽  
Marine Products

Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated “RFD-VV-M2” exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5–10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.

64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill
Keyword(s):  
Specific Detection ◽  
Species Specific ◽  
Urine Specimens

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

2019 ◽  
Author(s):  
Zacharias Thiel ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Subcellular Localization ◽  
Fluorescent Probe ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Fluorescent Sensor ◽  
Biological Functions ◽  
Localization Microscopy ◽  
Drug Activation ◽  
Nitroreductase Activity ◽  
Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

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