scholarly journals Label-Free, Dual-Analyte Electrochemical Biosensors: A New Class of Molecular-Electronic Logic Gates

2010 ◽  
Vol 132 (25) ◽  
pp. 8557-8559 ◽  
Author(s):  
Fan Xia ◽  
Xiaolei Zuo ◽  
Renqiang Yang ◽  
Ryan J. White ◽  
Yi Xiao ◽  
...  
Keyword(s):  
Logic Gates ◽  
Electrochemical Biosensors ◽  
Label Free ◽  
Molecular Electronic ◽  
New Class ◽  
Electronic Logic

Electrochemical biocomputing: a new class of molecular-electronic logic devices

Soft Matter ◽  
2013 ◽  
Vol 9 (29) ◽  
pp. 6571 ◽  
Author(s):  
Yongmei Jia ◽  
Ruixue Duan ◽  
Fan Hong ◽  
Boya Wang ◽  
Nannan Liu ◽  
...  
Keyword(s):  
Molecular Electronic ◽  
Logic Devices ◽  
New Class ◽  
Electronic Logic

scholarly journals Label-Free Electrochemical Test of Protease Interaction with a Peptide Substrate Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 199
Author(s):  
Anna Wcisło ◽  
Izabela Małuch ◽  
Paweł Niedziałkowski ◽  
Tadeusz Ossowski ◽  
Adam Prahl
Keyword(s):  
Gold Electrode ◽  
High Selectivity ◽  
Electrode Materials ◽  
Electrochemical Biosensors ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Peptide Substrate ◽  
Electrochemical Test ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method

Efficient deposition of biomolecules on the surface, maintaining their full activity and stability, is a most significant factor in biosensor construction. For this reason, more and more research is focused on the development of electrochemical biosensors that have the ability to electrically detect adsorbed molecules on electrode surface with high selectivity and sensitivity. The presented research aims to develop an efficient methodology that allows quantification of processes related to the evaluation of enzyme activity (proprotein convertase) using electrochemical methods. In this study we used impedance spectroscopy to investigate the immobilization of peptide substrate (Arg-Val-Arg-Arg) modified with 11-mercaptoundecanoic acid on the surface of gold electrode. Both the synthesis of the peptide substrate as well as the full electrochemical characteristics of the obtained electrode materials have been described. Experimental conditions, including concentration of peptide substrate immobilization, modification time, linker, and the presence of additional blocking groups have been optimized. The main advantages of the described method is that it makes it possible to observe the peptide substrate–enzyme interaction without the need to use fluorescent labels. This also allows observation of this interaction at a very low concentration. Both of these factors make this new technique competitive with the standard spectrofluorimetric method.

scholarly journals Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection Based on DNA Origami Nanostructures

ACS Omega ◽  
2019 ◽  
Vol 4 (6) ◽  
pp. 11025-11031 ◽  
Author(s):  
Shuo Han ◽  
Wenyan Liu ◽  
Shuo Yang ◽  
Risheng Wang
Keyword(s):  
Dna Origami ◽  
Electrochemical Biosensors ◽  
Label Free ◽  
Microrna Detection

scholarly journals Molecular logic gates: Encoded Multichromophore Response for Simultaneous Label-Free Detection Small 7/2010

Small ◽  
2010 ◽  
Vol 6 (7) ◽  
pp. NA-NA
Author(s):  
Constantin Pistol ◽  
Vincent Mao ◽  
Viresh Thusu ◽  
Alvin R. Lebeck ◽  
Chris Dwyer
Keyword(s):  
Logic Gates ◽  
Label Free ◽  
Molecular Logic ◽  
Molecular Logic Gates ◽  
Label Free Detection

scholarly journals Orthogonally modulated molecular transport junctions for resettable electronic logic gates

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Fanben Meng ◽  
Yves-Marie Hervault ◽  
Qi Shao ◽  
Benhui Hu ◽  
Lucie Norel ◽  
...  
Keyword(s):  
Molecular Transport ◽  
Logic Gates ◽  
Electronic Logic

Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

2015 ◽  
Vol 13 (43) ◽  
pp. 10604-10608 ◽  
Author(s):  
Maëva Reverte ◽  
Jean-Jacques Vasseur ◽  
Michael Smietana
Keyword(s):  
Nucleic Acids ◽  
Boronic Acid ◽  
Enzymatic Degradation ◽  
Label Free ◽  
New Class ◽  
Mismatch Detection ◽  
Modified Dna ◽  
Modified Nucleic Acids

Boronic acid modified DNA emerged as a new class of resistant oligonucleotides against enzymatic degradation. This property has been used to develop an enzyme-assisted label free method for mismatch detection.

scholarly journals Fluorogenic RNA Aptamers: A Nano-platform for Fabrication of Simple and Combinatorial Logic Gates

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 984 ◽  
Author(s):  
Victoria Goldsworthy ◽  
Geneva LaForce ◽  
Seth Abels ◽  
Emil Khisamutdinov
Keyword(s):  
Rna Binding ◽  
Fluorescent Dyes ◽  
Free Ligand ◽  
Logic Gates ◽  
Boolean Logic ◽  
Rna Aptamers ◽  
Nucleic Acid Detection ◽  
Rna Aptamer ◽  
Label Free ◽  
Half Adder

RNA aptamers that bind non-fluorescent dyes and activate their fluorescence are highly sensitive, nonperturbing, and convenient probes in the field of synthetic biology. These RNA molecules, referred to as light-up aptamers, operate as molecular nanoswitches that alter folding and fluorescence function in response to ligand binding, which is important in biosensing and molecular computing. Herein, we demonstrate a conceptually new generation of smart RNA nano-devices based on malachite green (MG)-binding RNA aptamer, which fluorescence output controlled by addition of short DNA oligonucleotides inputs. Four types of RNA switches possessing AND, OR, NAND, and NOR Boolean logic functions were created in modular form, allowing MG dye binding affinity to be changed by altering 3D conformation of the RNA aptamer. It is essential to develop higher-level logic circuits for the production of multi-task nanodevices for data processing, typically requiring combinatorial logic gates. Therefore, we further designed and synthetized higher-level half adder logic circuit by “in parallel” integration of two logic gates XOR and AND within a single RNA nanoparticle. The design utilizes fluorescence emissions from two different RNA aptamers: MG-binding RNA aptamer (AND gate) and Broccoli RNA aptamer that binds DFHBI dye (XOR gate). All computationally designed RNA devices were synthesized and experimentally tested in vitro. The ability to design smart nanodevices based on RNA binding aptamers offers a new route to engineer “label-free” ligand-sensing regulatory circuits, nucleic acid detection systems, and gene control elements.

Versatile and Programmable DNA Logic Gates on Universal and Label-Free Homogeneous Electrochemical Platform

2016 ◽  
Vol 88 (19) ◽  
pp. 9691-9698 ◽  
Author(s):  
Lei Ge ◽  
Wenxiao Wang ◽  
Ximei Sun ◽  
Ting Hou ◽  
Feng Li
Keyword(s):  
Logic Gates ◽  
Label Free

Berberine as a novel light-up i-motif fluorescence ligand and its application in designing molecular logic systems

2016 ◽  
Vol 52 (1) ◽  
pp. 179-182 ◽  
Author(s):  
Lijun Xu ◽  
Shanni Hong ◽  
Na Sun ◽  
Kewei Wang ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Logic Gates ◽  
Label Free ◽  
Molecular Logic ◽  
Free Dna ◽  
Logic Systems

Berberine is reported as a light-up fluorescence ligand for i-motif structures, which enables the development of label-free DNA-based logic gates.

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