scholarly journals Facile and Label-Free Electrochemical Biosensors for MicroRNA Detection Based on DNA Origami Nanostructures

ACS Omega ◽  
2019 ◽  
Vol 4 (6) ◽  
pp. 11025-11031 ◽  
Author(s):  
Shuo Han ◽  
Wenyan Liu ◽  
Shuo Yang ◽  
Risheng Wang
Keyword(s):  
Dna Origami ◽  
Electrochemical Biosensors ◽  
Label Free ◽  
Microrna Detection

scholarly journals Label-Free Electrochemical Test of Protease Interaction with a Peptide Substrate Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 199
Author(s):  
Anna Wcisło ◽  
Izabela Małuch ◽  
Paweł Niedziałkowski ◽  
Tadeusz Ossowski ◽  
Adam Prahl
Keyword(s):  
Gold Electrode ◽  
High Selectivity ◽  
Electrode Materials ◽  
Electrochemical Biosensors ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Peptide Substrate ◽  
Electrochemical Test ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method

Efficient deposition of biomolecules on the surface, maintaining their full activity and stability, is a most significant factor in biosensor construction. For this reason, more and more research is focused on the development of electrochemical biosensors that have the ability to electrically detect adsorbed molecules on electrode surface with high selectivity and sensitivity. The presented research aims to develop an efficient methodology that allows quantification of processes related to the evaluation of enzyme activity (proprotein convertase) using electrochemical methods. In this study we used impedance spectroscopy to investigate the immobilization of peptide substrate (Arg-Val-Arg-Arg) modified with 11-mercaptoundecanoic acid on the surface of gold electrode. Both the synthesis of the peptide substrate as well as the full electrochemical characteristics of the obtained electrode materials have been described. Experimental conditions, including concentration of peptide substrate immobilization, modification time, linker, and the presence of additional blocking groups have been optimized. The main advantages of the described method is that it makes it possible to observe the peptide substrate–enzyme interaction without the need to use fluorescent labels. This also allows observation of this interaction at a very low concentration. Both of these factors make this new technique competitive with the standard spectrofluorimetric method.

A Dual Functional Self-Enhanced Electrochemiluminescent Nanohybrid for Label-Free MicroRNA Detection

2021 ◽  
Author(s):  
Zhenqiang Ning ◽  
Erli Yang ◽  
Yongjun Zheng ◽  
Mengyuan Chen ◽  
Guoqiu Wu ◽  
...  
Keyword(s):  
Label Free ◽  
Dual Functional ◽  
Microrna Detection

Robust label-free microRNA detection using one million ISFET array

2018 ◽  
Vol 20 (2) ◽  
Author(s):  
Anurup Ganguli ◽  
Yoshihiko Watanabe ◽  
Michael T. Hwang ◽  
Jui-Cheng Huang ◽  
Rashid Bashir
Keyword(s):  
Label Free ◽  
Microrna Detection

scholarly journals A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green

RSC Advances ◽  
2019 ◽  
Vol 9 (56) ◽  
pp. 32906-32910 ◽  
Author(s):  
Honghong Wang ◽  
Hui Wang ◽  
Mai Zhang ◽  
Yuting Jia ◽  
Zhengping Li
Keyword(s):  
Malachite Green ◽  
Label Free ◽  
Microrna Detection

A label-free fluorescent aptamer-based biosensor is developed for the detection of microRNA by the fluorescence regulation of malachite green.

Trace and Label-Free MicroRNA Detection Using Oligonucleotide Encapsulated Silver Nanoclusters as Probes

2012 ◽  
Vol 84 (20) ◽  
pp. 8670-8674 ◽  
Author(s):  
Haifeng Dong ◽  
Shi Jin ◽  
Huangxian Ju ◽  
Kaihong Hao ◽  
Li-Ping Xu ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
Label Free ◽  
Microrna Detection

scholarly journals DNA nanotechnology approaches for microRNA detection and diagnosis

2019 ◽  
Vol 47 (20) ◽  
pp. 10489-10505 ◽  
Author(s):  
Arun Richard Chandrasekaran ◽  
Jibin Abraham Punnoose ◽  
Lifeng Zhou ◽  
Paromita Dey ◽  
Bijan K Dey ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Dna Origami ◽  
Great Promise ◽  
Dna Nanostructures ◽  
New Class ◽  
Advantages And Disadvantages ◽  
Critical Comparison ◽  
Microrna Detection ◽  
Disease Diagnostics ◽  
Detection And Diagnosis

Abstract MicroRNAs are involved in the crucial processes of development and diseases and have emerged as a new class of biomarkers. The field of DNA nanotechnology has shown great promise in the creation of novel microRNA biosensors that have utility in lab-based biosensing and potential for disease diagnostics. In this Survey and Summary, we explore and review DNA nanotechnology approaches for microRNA detection, surveying the literature for microRNA detection in three main areas of DNA nanostructures: DNA tetrahedra, DNA origami, and DNA devices and motifs. We take a critical look at the reviewed approaches, advantages and disadvantages of these methods in general, and a critical comparison of specific approaches. We conclude with a brief outlook on the future of DNA nanotechnology in biosensing for microRNA and beyond.

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