Transition State Analogue Structures of Human Phosphoglycerate Kinase Establish the Importance of Charge Balance in Catalysis

2010 ◽  
Vol 132 (18) ◽  
pp. 6507-6516 ◽  
Author(s):  
Matthew J. Cliff ◽  
Matthew W. Bowler ◽  
Andrea Varga ◽  
James P. Marston ◽  
Judit Szabó ◽  
...  
Keyword(s):  
Transition State ◽  
Phosphoglycerate Kinase ◽  
Charge Balance ◽  
Transition State Analogue

scholarly journals MgF3− and AlF4− transition state analogue complexes of yeast phosphoglycerate kinase

2017 ◽  
Vol 95 (2) ◽  
pp. 295-303
Author(s):  
Nicole E. McCormick ◽  
Stephanie M. Forget ◽  
Raymond T. Syvitski ◽  
David L. Jakeman
Keyword(s):  
Nmr Spectroscopy ◽  
Transition State ◽  
Chemical Shifts ◽  
Phosphoglycerate Kinase ◽  
Indirect Evidence ◽  
Metal Fluoride ◽  
Binding Affinities ◽  
Transition State Analogue ◽  
Transition State Analogues ◽  
Proton Chemical Shifts

The phospho-transfer mechanism of yeast phosphoglycerate kinase (PGK) has been probed through formation of trifluoromagnesate (MgF3−) and tetrafluoroaluminate (AlF4−) transition state analogue complexes and analyzed using 19F, 1H waterLOGSY and 1H chemical shift perturbation NMR spectroscopy. We observed the first 19F NMR spectroscopic evidence for the formation of metal fluoride transition state analogues of yeast PGK and also observed significant changes to proton chemical shifts of PGK in the presence, but not in the absence, of fluoride upon titration of ligands, providing indirect evidence of the formation of a closed ternary transition state. WaterLOGSY NMR spectroscopy experiments using an uncompetitive model were used in an attempt to measure ligand binding affinities within the transition state analogue complexes.

Crystal structure of cobra-venom phospholipase A2 in a complex with a transition-state analogue

Science ◽  
1990 ◽  
Vol 250 (4987) ◽  
pp. 1560-1563 ◽  
Author(s):  
S. White ◽  
D. Scott ◽  
Z. Otwinowski ◽  
M. Gelb ◽  
P. Sigler
Keyword(s):  
Crystal Structure ◽  
Transition State ◽  
Phospholipase A2 ◽  
Cobra Venom ◽  
Transition State Analogue

An Esterolytic Imprinted Polymer Prepared via a Silica-Supported Transition State Analogue

2004 ◽  
Vol 16 (14) ◽  
pp. 2745-2749 ◽  
Author(s):  
Kristian Lettau ◽  
Axel Warsinke ◽  
André Laschewsky ◽  
Klaus Mosbach ◽  
Ecevit Yilmaz ◽  
...  
Keyword(s):  
Transition State ◽  
Imprinted Polymer ◽  
Transition State Analogue

scholarly journals Transition State Analogue Inhibitors of 5′-Deoxyadenosine/5′-Methylthioadenosine Nucleosidase from Mycobacterium tuberculosis

Biochemistry ◽  
2017 ◽  
Vol 56 (38) ◽  
pp. 5090-5098 ◽  
Author(s):  
Hilda A. Namanja-Magliano ◽  
Gary B. Evans ◽  
Rajesh K. Harijan ◽  
Peter C. Tyler ◽  
Vern L. Schramm
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Transition State ◽  
Transition State Analogue ◽  
Transition State Analogue Inhibitors
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