scholarly journals DNA Strand Damage Product Analysis Provides Evidence That the Tumor Cell-Specific Cytotoxin Tirapazamine Produces Hydroxyl Radical and Acts as a Surrogate for O2

2007 ◽  
Vol 129 (42) ◽  
pp. 12870-12877 ◽  
Author(s):  
Goutam Chowdhury ◽  
Venkatraman Junnotula ◽  
J. Scott Daniels ◽  
Marc M. Greenberg ◽  
Kent S. Gates
Keyword(s):  
Tumor Cell ◽  
Hydroxyl Radical ◽  
Product Analysis ◽  
Dna Strand

scholarly journals Effect of Plant Phenolics on the Formation of the Spin-Adduct of Hydroxyl Radical and the DNA Strand Breaking by Hydroxyl Radical.

1996 ◽  
Vol 19 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Kazuyuki HIRAMOTO ◽  
Natsuko OJIMA ◽  
Ken-ichi SAKO ◽  
Kiyomi KIKUGAWA
Keyword(s):  
Hydroxyl Radical ◽  
Spin Adduct ◽  
Plant Phenolics ◽  
Dna Strand

scholarly journals Hydroxyurea for treatment of unresectable and recurrent meningiomas. I. Inhibition of primary human meningioma cells in culture and in meningioma transplants by induction of the apoptotic pathway

1997 ◽  
Vol 2 (4) ◽  
pp. E10
Author(s):  
Uwe M. H. Schrell ◽  
Michael G. Rittig ◽  
Marc Anders ◽  
Franklin Kiesewetter ◽  
Rolf Marschalek ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Cultures ◽  
Strand Break ◽  
Term Treatment ◽  
Dna Strand Break ◽  
Meningioma Cell ◽  
Dna Strand

Meningiomas, which invade intracranial bone structures and the adjacent connective tissue, are frequently unresectable because of their aggressive and recalcitrant growth behavior. They have a high recurrence rate, and in approximately 10% of these tumors there is an increased risk of malignancy. Significant morbidity and mortality rates associated with recurrent meningiomas demand nonsurgical approaches. To date, adjuvant hormonal treatment has not proven beneficial. The anticancer drug hydroxyurea was therefore tested for its potential use in the treatment of meningiomas. Early-passaged cell cultures were established from 20 different meningiomas. The addition of 5 X 10−4 and 10−3 M hydroxyurea over a period of 5 to 9 days resulted in a remarkable decrease in cell proliferation and even blocked tumor cell growth when compared with untreated cells. A significant arrest of meningioma cell growth in the S phase of the cell cycle was revealed on DNA flow cytometry. Electron micrographs of hydroxyurea-treated tumor cells showed ultrastructural features consistent with apoptosis, and light microscopy demonstrated DNA fragmentation by in situ DNA strand break labeling. Short-term treatment of meningioma cell cultures with hydroxyurea for 24 to 48 hours resulted in discrete oligonucleosomal fragments (DNA ladder), another characteristic sign of apoptosis. In addition to the in vitro studies, tissue from five different meningiomas was transplanted into nude mice followed by treatment with 0.5 mg/g body weight hydroxyurea over 15 days. In situ DNA strand break labeling demonstrated DNA fragmentation in distinct regions with different tumor cell densities in all hydroxyurea-treated meningioma transplants. These data provide evidence that hydroxyurea is a powerful inhibitor of meningioma cell growth, most likely by causing apoptosis in the tumor cells. Thus, hydroxyurea may be a suitable chemotherapeutic agent for the long-term treatment of unresectable or semi- to malignant meningiomas, or for preventing recurrent growth of meningiomas after resection.

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