New Poly(d-glucaramidoamine)s Induce DNA Nanoparticle Formation and Efficient Gene Delivery into Mammalian Cells

2004 ◽  
Vol 126 (24) ◽  
pp. 7422-7423 ◽  
Author(s):  
Yemin Liu ◽  
Laura Wenning ◽  
Matthew Lynch ◽  
Theresa M. Reineke
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Nanoparticle Formation ◽  
Efficient Gene

scholarly journals Efficient gene delivery into mammalian cells by baculovirus vector in vitro

2008 ◽  
Vol 24 (6) ◽  
pp. 508-512 ◽  
Author(s):  
I. N. Vagyna ◽  
O. V. Anopriyenko ◽  
O. A. Zaharuk ◽  
V. F. Gorchev ◽  
L. I. Strokovska ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Baculovirus Vector ◽  
Efficient Gene

Baculovirus vectors for efficient gene delivery and expression in mammalian cells

2010 ◽  
Vol 44 (3) ◽  
pp. 479-487 ◽  
Author(s):  
N. N. Koroleva ◽  
P. V. Spirin ◽  
A. V. Timokhova ◽  
P. M. Rubtzov ◽  
S. N. Kochetkov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Efficient Gene ◽  
Expression In Mammalian Cells

Baculovirus as a highly efficient gene delivery vector for the expression of hepatitis delta virus antigens in mammalian cells

2005 ◽  
Vol 89 (4) ◽  
pp. 464-473 ◽  
Author(s):  
Kuei-Chun Wang ◽  
Jaw-Chin Wu ◽  
Yao-Chi Chung ◽  
Yi-Chen Ho ◽  
Margaret Dah-Tsyr Chang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hepatitis Delta Virus ◽  
Mammalian Cells ◽  
Hepatitis Delta ◽  
Gene Delivery Vector ◽  
Highly Efficient ◽  
Delivery Vector ◽  
Efficient Gene ◽  
Virus Antigens ◽  
Delta Virus

scholarly journals Effective transduction of osteogenic sarcoma cells by a baculovirus vector

2003 ◽  
Vol 84 (3) ◽  
pp. 697-703 ◽  
Author(s):  
Sun U. Song ◽  
Seok-Hwan Shin ◽  
Soon-Ki Kim ◽  
Gwang-Seong Choi ◽  
Woo-Chul Kim ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cell Lines ◽  
Reporter Gene ◽  
Mammalian Cells ◽  
Osteogenic Sarcoma ◽  
Transduction Efficiency ◽  
Sarcoma Cell ◽  
Efficient Gene ◽  
High Level ◽  
Sarcoma Cells

Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus vectors containing a lacZ reporter gene under the control of either a cytomegalovirus or Rous sarcoma virus promoter. The expression of β-galactosidase protein, assessed by X-Gal staining and β-galactosidase ELISA, demonstrated an extremely high level of transduction efficiency in some osteogenic sarcoma cell lines, such as U-2OS, Saos-2 and Saos-LM2. These human osteogenic sarcoma cell lines showed levels of β-galactosidase expression 5–40 times greater than HepG2 cells, which were previously thought to be the mammalian cells most susceptible to baculovirus-mediated gene delivery. The level of acetylated histone proteins in these tumour lines did not correlate well with the high level of reporter gene expression. These results strongly suggest that some osteogenic sarcoma cells are highly susceptible to baculovirus-mediated gene delivery and that a baculovirus-derived vector is an efficient gene delivery vehicle into human osteogenic sarcoma cells.

Efficient Gene Delivery Using Anionic Liposome-Complexed Polyplexes (LPDII)

2000 ◽  
Vol 20 (5) ◽  
pp. 419-432 ◽  
Author(s):  
Wenjin Guo ◽  
Robert J. Lee
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Efficient Gene ◽  
Anionic Liposomes ◽  
Cholesteryl Hemisuccinate

Synthetic gene transfer vectors based on polyplexes complexed to anionic liposomes (LPDII vectors) were characterized for their transfection efficiency in cultured mammalian cells. The effects of polycation to DNA ratio, lipid to DNA ratio, choice of polycation and lipid composition were systematically evaluated in human oral carcinoma KB cells, using a luciferase reporter gene. For LPDII formulations containing poly-L-lysine and dioeoylphosphatidylethanolamine/cholesteryl hemisuccinate (DOPE/CHEMS) anionic liposomes, at a constant lipid to DNA ratio, an increase in the polycation/DNA (N/P) ratio resulted in an increase in transfection activity. Meanwhile, the optimal lipid to DNA ratio for efficient gene delivery was influenced by the N/P ratio used, and was increased at higher N/P ratios. For the DNA condensing agent, poly-L-lysine could be replaced by polyethylenimine (PEI) as the DNA condensing agent in the formulations. For the lipidic components, CHEMS could be replaced by other anioniclipids including oleic acid, dicetylphosphate and phosphatidylserine, but DOPE, a fusogenic helper lipid, could not be replaced by dioleolyphosphatidylcholine. LPDII formulation showed significantly less cytotoxicity compared to the commonly used cationic lipsomes or PEI mediated transfection and several cell lines were transfected with high efficiency. LPDII vectors avoid the use of toxic cationic lipids and may have potential application in gene therapy.

Sign in / Sign up

Export Citation Format

Share Document