Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus replicon

2009 ◽  
Vol 11 (11) ◽  
pp. 1030-1038 ◽  
Author(s):  
Yongfei Pan ◽  
Qian Zhao ◽  
Liurong Fang ◽  
Rui Luo ◽  
Huanchun Chen ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Semliki Forest Virus ◽  
Recombinant Baculovirus ◽  
Cytomegalovirus Promoter ◽  
Efficient Gene

New Poly(d-glucaramidoamine)s Induce DNA Nanoparticle Formation and Efficient Gene Delivery into Mammalian Cells

2004 ◽  
Vol 126 (24) ◽  
pp. 7422-7423 ◽  
Author(s):  
Yemin Liu ◽  
Laura Wenning ◽  
Matthew Lynch ◽  
Theresa M. Reineke
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Nanoparticle Formation ◽  
Efficient Gene

scholarly journals Efficient gene delivery into mammalian cells by baculovirus vector in vitro

2008 ◽  
Vol 24 (6) ◽  
pp. 508-512 ◽  
Author(s):  
I. N. Vagyna ◽  
O. V. Anopriyenko ◽  
O. A. Zaharuk ◽  
V. F. Gorchev ◽  
L. I. Strokovska ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Baculovirus Vector ◽  
Efficient Gene

scholarly journals Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1306
Author(s):  
Ha Youn Shin ◽  
Hanul Choi ◽  
Nahyun Kim ◽  
Nayoung Park ◽  
Heesun Kim ◽  
...  
Keyword(s):  
Immune Response ◽  
Gene Delivery ◽  
Mammalian Cells ◽  
Vaccine Development ◽  
Recombinant Baculovirus ◽  
Cytokine Signaling ◽  
Rna Seq ◽  
Immune Response Genes ◽  
Genome Wide ◽  
Baculovirus Infection

Baculovirus expression systems have been widely used to produce recombinant mammalian proteins owing to the lack of viral replication in vertebrates. Although several lines of evidence have demonstrated impacts of baculovirus infection in mammalian hosts, genome-wide effects have not been fully elucidated. Here, we provide comparative transcriptome profiles of baculovirus and host-immune response genes in recombinant baculovirus-infected mammalian and insect cells. Specifically, to decipher the impacts of baculovirus infection in mammalian cells, we conducted total RNA-seq on human 293TT cells and insect Sf9 cells infected with recombinant baculovirus. We found that baculovirus genes were rarely expressed under the control of baculoviral promoters in 293TT cells. Although some baculovirus early genes, such as PE38 and IE-01, showed limited expression in 293TT cells, baculoviral late genes were mostly silent. We also found modest induction of a small number of mammalian immune response genes associated with Toll-like receptors, cytokine signaling, and complement in baculovirus-infected 293TT cells. These comprehensive transcriptome data will contribute to improving recombinant baculovirus as tools for gene delivery, gene therapy, and vaccine development.

Baculovirus vectors for efficient gene delivery and expression in mammalian cells

2010 ◽  
Vol 44 (3) ◽  
pp. 479-487 ◽  
Author(s):  
N. N. Koroleva ◽  
P. V. Spirin ◽  
A. V. Timokhova ◽  
P. M. Rubtzov ◽  
S. N. Kochetkov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Efficient Gene ◽  
Expression In Mammalian Cells

scholarly journals Baculovirus as a Tool for Gene Delivery and Gene Therapy

Viruses ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 510 ◽  
Author(s):  
Chikako Ono ◽  
Toru Okamoto ◽  
Takayuki Abe ◽  
Yoshiharu Matsuura
Keyword(s):  
Gene Delivery ◽  
Mammalian Cells ◽  
Recombinant Baculovirus ◽  
Foreign Gene ◽  
Type I ◽  
Adjuvant Activity ◽  
Baculovirus Infection ◽  
Immunodeficiency Virus ◽  
Host Innate Immune Response ◽  
Infected Cells

Based on its ability to express high levels of protein, baculovirus has been widely used for recombinant protein production in insect cells for more than thirty years with continued technical improvements. In addition, baculovirus has been successfully applied for foreign gene delivery into mammalian cells without any viral replication. However, several CpG motifs are present throughout baculoviral DNA and induce an antiviral response in mammalian cells, resulting in the production of pro-inflammatory cytokines and type I interferon through a Toll-like receptor (TLR)-dependent or -independent signaling pathway, and ultimately limiting the efficiency of transgene expression. On the other hand, by taking advantage of this strong adjuvant activity, recombinant baculoviruses encoding neutralization epitopes can elicit protective immunity in mice. Moreover, immunodeficient cells, such as hepatitis C virus (HCV)- or human immunodeficiency virus (HIV)-infected cells, are more susceptible to baculovirus infection than normal cells and are selectively eliminated by the apoptosis-inducible recombinant baculovirus. Here, we summarize the application of baculovirus as a gene expression vector and the mechanism of the host innate immune response induced by baculovirus in mammalian cells. We also discuss the future prospects of baculovirus vectors.

Baculovirus as a highly efficient gene delivery vector for the expression of hepatitis delta virus antigens in mammalian cells

2005 ◽  
Vol 89 (4) ◽  
pp. 464-473 ◽  
Author(s):  
Kuei-Chun Wang ◽  
Jaw-Chin Wu ◽  
Yao-Chi Chung ◽  
Yi-Chen Ho ◽  
Margaret Dah-Tsyr Chang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hepatitis Delta Virus ◽  
Mammalian Cells ◽  
Hepatitis Delta ◽  
Gene Delivery Vector ◽  
Highly Efficient ◽  
Delivery Vector ◽  
Efficient Gene ◽  
Virus Antigens ◽  
Delta Virus
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